Fungal glucan oligomer chrome complex and preparation method thereof

A technology of chromium complex and oligoglucose, which is applied in the field of chromium oligoglucose and its preparation, can solve the problems of harsh reaction conditions, large ratio differences, and low bioavailability, and achieve mild synthesis conditions, The effect of short production cycle and low molecular weight

Inactive Publication Date: 2015-03-11
BINZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] To sum up, there are many kinds of organic chromium with biological activity. However, because their solubility and intestinal absorption ratio vary greatly, their biological activities vary. Some have poor solubility and low bioavailability, and some anionic complexes There are adverse effects on the human body, and the reaction conditions are harsh in some preparation processes, which makes the development of new organic chromium complexes (chelated compounds) with good solubility, easy absorption and strong biological activity, high bioavailability, no side effects or low side effects. substances), becoming an important new growth point of chromium compounds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation of Sclerotinia β-glucooligosaccharide

[0054] Suspend 15g of Sclerotin glucan in 100mL of water, stir to fully swell, adjust the pH to 4-5, add 30mg of β-glucanase when heated to 50°C, and hydrolyze for 1-2 hours under stirring; then add glucose Polysaccharide 10g, keep stirring for 1-2h, the solution becomes thinner and gradually transparent; adjust the pH to 7-8, add 20mg of neutral protease, keep warm for 2-4h; raise the temperature to 90°C, inactivate the enzyme activity; centrifuge at 6000r / min for 20min, Ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 10 kDa to collect the filtrate, and then ultrafiltration with a nanofiltration membrane with a molecular weight cut-off of 200 Dalton to collect the retentate; that is, oligoglucose with a weight average molecular weight of 2528 Dalton. The gluco-oligosaccharide solution contains 22.51% (w / v) of β-gluco-oligosaccharide.

Embodiment 2

[0056] Synthesis and purification of β-glucooligosaccharide chromium from Sclerotinia sp.

[0057] Sclerotinia β-glucooligosaccharide solution 100mL, containing 15.37% (w / v) of glucooligosaccharide with a weight average molecular weight of 2240Dalton, adjusted the pH value to 12 with 10mol / L NaOH, heated to 75°C, and added dropwise 2mol / L During this period, use 2mol / L NaOH to adjust the pH to stabilize the pH value at about 12. When green flocculent precipitates appear in the solution, stop adding chromium chloride and NaOH dropwise, and the reaction solution continues to be heated at 75°C. Keep warm for 1h. Ultrafiltration with a nanofiltration membrane with a molecular weight cut-off of 200 Daltons was used to collect the retentate; further purified by 200-300 mesh silica gel column chromatography, and then freeze-dried to obtain 8.6 g of Sclerotinia β-glucooligosaccharide chromium. The chromium yield of β-glucooligosaccharide is 56%, and the chromium content is 7.43%w / w. ...

Embodiment 3

[0066] Preparation of Aureobasidium pullulans α-gluco-oligosaccharide

[0067] Suspend 10g of pullulan in 100mL of water, stir to fully swell, adjust the pH to 4-5, heat at 55°C, add 10mg of α-1,6-glucanase, hydrolyze for 2 hours under stirring; then add 5g of dextran , 10 mg of α-amylase was continuously stirred for 1-2 hours, the solution became thinner and gradually became transparent; adjust the pH to 7-8, add 15 mg of alkaline protease, and keep warm for 2-4 hours; raise the temperature to 100°C to inactivate the enzyme; centrifuge at 6000r / min After 20 minutes, use ultrafiltration with a molecular weight cut-off of 6000 Dalton to collect the filtrate, and then use a nanofiltration membrane with a molecular weight cut-off of 300 Dalton to collect the retentate; that is, the gluco-oligosaccharide with a weight average molecular weight of 1650 Dalton. The gluco-oligosaccharide solution contains 12.6% (w / v) of α-gluco-oligosaccharide.

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Abstract

The invention relates to the technical fields of bioengineering and food medicine, and particularly provides a glucan oligomer chrome complex prepared by using fungal glucan oligomer as a ligand compound and trivalent chrome as coordination center ions and a preparation method thereof. The prepared glucan oligomer chrome complex has the advantages of proper chrome content, high stability and favorable water solubility, can be easily absorbed, and is free of free Cr<6+> ions; and the deionized ligand compound has the advantages of no toxic or side effect and high bioactivity, and is an active substance with GTF like function and immunoregulation action.

Description

technical field [0001] The invention relates to the technical field of bioengineering, and specifically provides a glucooligosaccharide chromium complex prepared by using fungal glucooligosaccharide as a ligand compound and using trivalent chromium as a coordination center ion and a preparation method thereof. Background technique [0002] Chromium is an important trace element that has been widely studied and the conclusions are relatively consistent. The content of this trace element is significantly reduced in diabetic patients. The reason is that trivalent chromium can form glucose tolerance factor (glucose tolerance factor) in the human body. , GTF), cooperate with insulin to participate in the metabolism of sugar and lipid in human body. 1950s, Cr 3+ It has been proven to be the main active ingredient of GTF, and was identified as an essential trace element for the human body in the 1980s. The physiological function of chromium is mainly based on Cr 3+ The form of G...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07H3/04C07H3/06C07H1/00C12P19/22C12P19/14A61P3/10
Inventor 王宝琴徐泽平
Owner BINZHOU UNIV
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