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Method and apparatus of controlling supplement of nutrient salts to culture microalgae based on optical density feedback

A feedback control and optical density technology, applied in the direction of microorganism-based methods, enzymology/microbiology devices, photobioreactors, etc., can solve problems such as low yield and affecting production control

Active Publication Date: 2013-05-15
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the blindness of nutrient supplementation in the existing microalgae cultivation, which affects production control and low yield, and provides a method for cultivating microalgae based on optical density feedback control supplementation of nutrient salts. By monitoring the optical density (optical density, OD) change of the culture medium at a certain wavelength during the cultivation process, the OD feedback is used to control the addition of nutrients, thereby simplifying the production process and increasing the algae biomass or products (total lipid, protein, Method for Feedback Feeding Culture Microalgae of Polysaccharide, etc.) Content

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  • Method and apparatus of controlling supplement of nutrient salts to culture microalgae based on optical density feedback
  • Method and apparatus of controlling supplement of nutrient salts to culture microalgae based on optical density feedback
  • Method and apparatus of controlling supplement of nutrient salts to culture microalgae based on optical density feedback

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Embodiment 1

[0063] The microalgal species used was Spirulina platensis (Spirulina platensis, 439, freshwater algae species bank of the Typical Culture Collection Committee of the Chinese Academy of Sciences), and the above-mentioned algae species were used for fed-batch culture based on OD feedback control to supplement nutrients.

[0064] The composition of culture medium and nutrient salt feeding solution is the same as that of Comparative Example 1.

[0065] Microalgae cultivation device is 1000m 2 The open raceway pool uses an impeller stirring device to drive the flow and mixing of the culture solution, and the thickness of the culture solution layer is 15cm. See Nutrient Control Devices figure 1 , wherein the optical density sensor is a commercially available online OD sensor (GX-2, Nanning Anshengda Technology Co., Ltd.), the detection wavelength is 650nm, and the controller is an automatic controller. When the increment of the OD value exceeds the increment of the set OD value, ...

Embodiment 2

[0068] The algae species, culture medium, microalgae incubator and nutrient salt control device are the same as in Example 1. The above-mentioned medium is not sterilized, and is directly prepared in the raceway pool before adding algae species. The initial algae cell concentration is 0.15g / L, the temperature is natural (22-37°C), the light is natural light, and the light intensity is 575-1200μmol / m 2 .s, the flow rate of the culture medium is 15m / min. Nutrient salt feeding liquid is the mixed aqueous solution of ammonium sulfate, dipotassium hydrogen phosphate, wherein the concentration of ammonium sulfate is 2.5mol / L, and the concentration of dipotassium hydrogen phosphate is 0.21mol / L (the concentration ratio of ammonium sulfate and dipotassium hydrogen phosphate Determined by the ratio of the consumption of the two in the constant-speed pulse fed-batch culture of Comparative Example 1).

[0069] During the cultivation process, the OD value of the initial culture medium wa...

Embodiment 3

[0075] The microalgae used is Spirulina platensis (Spirulina platensis, 439, freshwater algae species bank of Type Culture Collection Committee, Chinese Academy of Sciences). The above-mentioned algae species were used for fed-batch culture based on OD feedback control of nutrient supplementation.

[0076] The composition of the medium is: sodium bicarbonate 8.4g / L, sodium nitrate 0.5g / L, phosphoric acid 0.1g / L, sodium chloride 1.0g / L, potassium chloride 1.0g / L, magnesium sulfate 0.1g / L, Ferrous sulfate heptahydrate 0.01g / L, disodium EDTA 0.08g / L, calcium chloride 0.04g / L. Nutrient salt feeding liquid is the mixed aqueous solution of sodium nitrate, phosphoric acid and magnesium sulfate, and wherein the concentration of sodium nitrate is 1.5mol / L, and the concentration of phosphoric acid is 0.065mol / L, and the concentration of magnesium sulfate is 0.005mol / L (sodium nitrate, The concentration ratio of phosphoric acid and magnesium sulfate is determined by the ratio of the con...

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Abstract

The invention relates to a field of culturing microalgae, concretely to a method and an apparatus of controlling supplement of nutrient salts to culture microalgae based on optical density feedback. According to the method, an adding amount of the nutrient salts liquid is calculated through a formula (I) shown in the description, wherein alpha (g / L) is a conversion coefficient of an increment of an optical density (OD) value and an increment of microalgae cells, that is, [delta]X = [alpha][delta]OD, beta (mol / g) is a proportion coefficient of the increment of the microalgae cells and consumption amount of the nutrient salts, and [delta]OD is the increment of the optical density (OD) value of a culture medium. The increment of the optical density (OD) value of the culture medium is preset, and when the increment of the optical density of the culture medium reaches the set value, the adding amount of the nutrient salts liquid is calculated through the formula (I). Or, a time interval of detecting the optical density (OD) value is set, the increment of the optical density (OD) value of the culture medium is detected in the time interval, and the adding amount of the nutrient salts liquid is calculated through the formula (I) in accordance with the detected optical density (OD) value. The method can relatively accurately control the adding amount of the nutrient salts.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular, the invention relates to a method and a device for cultivating microalgae based on optical density feedback control and nutrient supplementation. Background technique [0002] Microalgae can use CO 2 , light, water and nutrients for photosynthesis to synthesize organic substances and release oxygen. Microalgae cells are rich in protein, carbohydrates, lipids and pigments, etc., and have been widely used in the fields of food, feed, medicine, fine chemicals and dyes. With the depletion of petroleum and other energy sources, the new energy technology of using microalgae to produce hydrogen, oil, and olefins has attracted great attention. [0003] Low-cost, large-scale cultivation is the key technology to realize the industrialization of microalgae. There are two main ways to reduce the cost of microalgae production, one is to reduce the cost of raw materials for cultivation, ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/00C12M1/36C12M1/34C12R1/89
CPCC12M21/02C12M41/32C12M41/36
Inventor 丛威鲍亦璐张东梅温树梅刘明
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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