Traditional Chinese medicine perfusate containing total alkaloids of Sophora fructus for treating dairy cow mastitis and endometritis and preparation method thereof
A technology of endometritis and Sophora sophora, which is applied in the field of traditional Chinese medicine perfusate containing total alkaloids of Sophora sophora, can solve the problems of reducing milk production years of dairy cows, easy recurrence, high incidence of mastitis and endometritis in dairy cows, and achieves Conducive to stability and quality controllability, recovery of overall function and body condition, and less likely to produce drug resistance
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Embodiment 1
[0100] The preparation method of the traditional Chinese medicine perfusate containing total alkaloids of Sophora Sophorae comprises the following steps:
[0101] 1) Grind the seeds of Sophora sophora, add ethanol aqueous solution for ultrasonic extraction, recover ethanol after filtration, add 2% hydrochloric acid to adjust the pH, add ether to wash twice, obtain the filtrate precipitate, then add chloroform to extract the precipitate, recover chloroform under reduced pressure to obtain Sophora sophora total alkali;
[0102] 2) Add water for injection to 3 g of the total alkaloids of Sophora sophora obtained in step 1) to make the volume to 100 ml, sterilize with circulating steam for 30 minutes, and fill under aseptic conditions to obtain the finished traditional Chinese medicine perfusate.
Embodiment 2
[0104] The preparation method of the traditional Chinese medicine perfusate containing total alkaloids of Sophora Sophorae comprises the following steps:
[0105] 1) Grind the seeds of Sophora sophora, add ethanol aqueous solution for ultrasonic extraction, recover ethanol after filtration, add 2% hydrochloric acid to adjust the pH, add ether to wash twice, obtain the filtrate precipitate, then add chloroform to extract the precipitate, recover chloroform under reduced pressure to obtain Sophora sophora total alkali;
[0106] 2) Add water for injection to 8 g of total alkaloids obtained in step 1) to make the volume to 100 ml, sterilize with circulating steam for 40 min, and fill under aseptic conditions to obtain the finished traditional Chinese medicine perfusate.
Embodiment 3
[0108] The preparation method of the traditional Chinese medicine perfusate containing total alkaloids of Sophora Sophorae comprises the following steps:
[0109] 1) Grind the seeds of Sophora sophora, add ethanol aqueous solution for ultrasonic extraction, recover ethanol after filtration, add 2% hydrochloric acid to adjust the pH, add ether to wash twice, obtain the filtrate precipitate, then add chloroform to extract the precipitate, recover chloroform under reduced pressure to obtain Sophora sophora total alkali;
[0110] 2) Add water for injection to 6 g of the total alkaloids of Sophora sophorae obtained in step 1) to make the volume to 100 ml, sterilize with circulating steam for 35 minutes, and fill under aseptic conditions to obtain the finished traditional Chinese medicine perfusate.
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