Antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof

A technology of tobacco bacterial wilt and antagonistic bacteria, applied in the direction of application, bacteria, fungi, etc., can solve problems such as relatively large impact, unsatisfactory control effect, and unachievable crop rotation measures

Inactive Publication Date: 2013-06-12
SHAOWU BRANCH OF NANPING TOBACCO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, agricultural measures such as disease-resistant varieties and crop rotations are mainly used in production to prevent and control tobacco soil-borne diseases. However, because disease-resistant varieties require diversification of disease-resistant genes and are greatly aff

Method used

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  • Antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof
  • Antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof
  • Antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1. Isolation and screening of antagonistic bacteria against tobacco bacterial wilt

[0039] 1) Isolation of pathogenic bacteria

[0040] Before isolating the germs, rinse the tobacco stems, and then disinfect the tobacco stems and a knife with 75% alcohol. Then, under aseptic conditions, use a knife to cut open the diseased spots on the stems of the tobacco plants and remove the vascular tissue of the affected parts. Cut into several small pieces, use sterilized tweezers to take the tobacco pieces and place them in a beef extract peptone bacteria plate petri dish for culture, put 5 pieces in each dish, repeat 3 dishes, culture temperature 32℃, culture time 48h. When the bacterial wilt tobacco block is in contact with the culture medium, a white fluid colony will grow and the small bacteria will be dispersed outward, and it will be inoculated into the beef extract peptone for storage.

[0041] The photomicrograph shows that the bacterial wilt pathogen is rod-shaped, wi...

Example Embodiment

[0056] Example 2. Morphological observation

[0057] The initial colony of QJ-1 bacteria is flat, milky white, smooth and opaque.

[0058] Ty-3 bacteria have white hyphae at the initial stage, and then produce dark brown or dark brown spores with abundant spores.

Example Embodiment

[0059] Example 3 Identification and classification of antagonistic bacteria

[0060] The identification result of the Institute of Microbiology, Chinese Academy of Sciences:

[0061] 1. QJ-1 is Bacillus cereus.

[0062] 1) Cell morphology and physical and chemical test results

[0063] Gram-positive, the cell is rod-shaped, the cell diameter is greater than 1μm, spores are formed, and the spores are not enlarged; VP test, methyl red test, starch hydrolysis, casein decomposition, gelatin hydrolysis, contact enzyme, oxidase, nitrate reduction reaction , Utilization of citrate is positive; acid production reaction is shown as positive for glucose, negative for xylose, L-arabinose, mannitol, and lactose.

[0064] 2) 16S rRNA gene sequence determination results

[0065] GCAGTCGAGC GATGGATTAA GAGCTTGCTC TTATGAAGTT AGCGGCGGAC

[0066] GGGTGAGTAA CACGTGGGTA ACCTGCCCAT AAGACTGGGA TAACTCCGGG

[0067] AAACCGGGGC TAATACCGGA TAACATTTTG AACTGCATGG TTCGAAATTG

[0068] AAAGGCGGCT TCGGCTGCCA CTTATGGATG GAC...

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Abstract

The invention discloses antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof. The antagonistic bacteria are Bacillus cereus QJ-1 and Aspergillus niger Ty-3. Specifically, the preservation unit of QJ-1 is China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation date is July 5, 2012, and the preservation number is: CCTCC, NO:M2012271; and the preservation unit of Ty-3 is China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation date is July 8, 2011, and the preservation number is CCTCC NO:M2011241. The antagonistic bacteria, i.e. the Bacillus cereus QJ-1 and Aspergillus niger Ty-3 provided in the invention can be combined into a microbial agent. The microbial agent and an organic material can be fermented into a microbial organic fertilizer, which can provide effective nutrition and functional bacteria, and realize effective prevention and treatment of tobacco bacterial wilt.

Description

technical field [0001] The invention relates to antagonistic bacteria for preventing and treating tobacco bacterial wilt, and also relates to the application of the antagonistic bacteria in preventing and treating tobacco soil-borne diseases, belonging to the field of biological control. Background technique [0002] Tobacco bacterial wilt is an important soil-borne disease of tobacco, which usually infects the roots of tobacco, causes diseases of crop roots and even the whole plant, and causes significant economic losses. Tobacco bacterial wilt is a systemic infectious disease caused by Ralstonia solanacearum. Once the tobacco plant is infected, the whole plant often dies. The damage is often devastating, so it often causes significant economic losses to tobacco production. . The pathogenic bacteria of tobacco bacterial wilt mainly survives the winter in the soil along with plant residues, and can also survive the winter in seeds or other hosts in the field. The soil with ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/00A01P1/00C05F11/08C12R1/085C12R1/685
Inventor 李红丽卢阿虔王岩
Owner SHAOWU BRANCH OF NANPING TOBACCO
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