Antarctic ice algae CPD photolyase, and coding gene, expression vector and application thereof
A technology of photorepair enzymes and Antarctic ice algae, applied in the field of biology, can solve the problems of research difficulties such as the separation and purification of biological photorepair enzymes and in vitro activity, and the lack of photorepair enzymes. benefit effect
Active Publication Date: 2013-06-19
QINGDAO HENGSHENG BIOLOGICAL PHARMA TECH DEV
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The invention discloses an Antarctic ice algae CPD photolyase which is a protein (a) or (b): (a) amino acid sequence disclosed as SEQ ID NO:2; or (b) (a)-derived protein with CPD photolyase activity subjected to substitution, deletion or addition of 1-10 amino acid residues on the amino acid sequence in (a). The invention also discloses a coding gene and an expression vector of the Antarctic ice algae CPD photolyase, and application of the Antarctic ice algae CPD photolyase in the related fields of cosmetics and biomedicine. The invention clones the Antarctic ice algae CPD photolyase gene for the first time, successfully clones the Antarctic ice algae CPD photolyase gene into the expression vector to obtain abundant CPD photolyase, applies the CPD photolyase to the related fields of cosmetics, biomedicine and the like, and can actively restore diseases caused by ultraviolet rays, thereby bringing about huge social benefit and having important meanings.
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Property | Measurement | Unit |
Sub-mass | 64.3 | |
tensile | MPa | |
Particle size | Pa | |
strength | 10 |
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