Broccoli leftover extractive and preparation method thereof

A technology for broccoli and leftovers, which is applied in the field of extracting sulforaphane and flavonoids, can solve the problems of high loss of effective components, complicated operation, environmental pollution, etc., and achieves improved operability, mild extraction conditions and broad market prospects. Effect

Inactive Publication Date: 2013-06-26
湘西新金凤凰农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, broccoli scraps were mainly used as animal feed, or were treated as garbage, which was not of high value. In fact, the components of broccoli scraps and broccoli vegetables are almost the same, so the treatment of broccoli scraps This method not only caused a serious waste of resources, but also caused environmental pollution.
[0004] In recent years, broccoli and its leftover extracts have been gradually accepted and favored by consumers. Broccoli and its leftover extracts are rich in various active ingredients such as sulforaphane and flavonoids, which have anti-cancer and anti-cancer properties. , anti-oxidation, anti-aging and other multiple effects, widely used in health food, medicine and chemical industry and other fields, there are also some researches on the preparation process of broccoli and its leftover extracts, but most of them have high cost, complicated operation and low yield and the problem of high loss of active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Pretreatment of broccoli scraps. After pulping the broccoli scraps, add 1.5 times the amount of enzymatic hydrolysis solution for 3 hours at a temperature of 45 degrees. Ultrasonic waves are assisted during the enzymatic hydrolysis process. The preparation method of the enzymatic solution is: dissolve 3 grams of compound enzyme It is obtained in 500ml of buffer solution with a pH value of 4.9, wherein the compound enzyme preparation consists of 68% cellulase mass fraction and the rest is pectinase.

[0015] 2. Extraction of broccoli sulforaphane and flavonoids. After enzymatic hydrolysis, continue to add 2.8 times the weight of broccoli scraps, 78% ethanol for reflux extraction for 3 hours, filter after extraction, then add 4 times the amount of drinking water for reflux extraction for 2.5 hours, reflux twice Microwaves are used to assist in the extraction process, the extracts obtained by two reflux extractions are combined, the alcohol is recovered under reduced p...

Embodiment 2

[0018] 1. Pretreatment of broccoli scraps. After pulping the broccoli scraps, add 4.5 times the amount of enzymatic hydrolysis solution at a temperature of 50 degrees for 6.8 hours of enzymolysis, and assist ultrasonic waves during the enzymatic hydrolysis process. The preparation method of the enzymolysis solution is: dissolve 13 grams of compound enzyme preparation It is obtained in 500ml of buffer solution with a pH value of 6.1, wherein the compound enzyme preparation consists of 53% cellulase mass fraction and the rest is pectinase.

[0019] 2. Extraction of broccoli sulforaphane and flavonoids. After enzymatic hydrolysis, continue to add 3.8 times the weight of broccoli scraps, 95% ethanol for reflux extraction for 4.2 hours, filter after extraction, then add 4.8 times the amount of drinking water for reflux extraction for 4.1 hours, reflux twice Microwaves are used to assist in the extraction process, the extracts obtained by two reflux extractions are combined, the al...

Embodiment 3

[0022] 1. Pretreatment of broccoli scraps. After pulping the broccoli scraps, add 3 times the amount of enzymatic hydrolysis solution for 5 hours at a temperature of 48 degrees. The preparation method of the enzymolysis solution is: dissolve 8 grams of compound enzyme preparation in 500ml of pH value of 5.6 It is obtained in buffer solution, wherein the compound enzyme preparation is composed of cellulase mass fraction of 58%, and the rest is pectinase.

[0023] 2. Extraction of broccoli sulforaphane and flavonoids. After enzymatic hydrolysis, continue to add 3.5 times the weight of broccoli leftovers, 85% ethanol for reflux extraction for 4 hours, filter after extraction, then add 4.5 times the amount of drinking water for reflux extraction for 3.5 hours, and combine twice The extracted extract was extracted, the alcohol was recovered under reduced pressure and continued to be concentrated to a paste, and then vacuum-dried to obtain the broccoli leftover extract.

[0024] T...

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PUM

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Abstract

The invention discloses a broccoli leftover extractive and a preparation method thereof. The method comprises the following steps of: (1) pulping broccolis and carrying out ultrasonic-assisted enzymolysis on pulp; and (2) extracting broccoli raphanin and flavonoid compounds, respectively adding ethanol and water to carry out microwave-assisted reflowing extraction, combining extraction solutions obtained by two times of reflowing extraction, recycling alcohol by decompressing, continually concentrating the recovered alcohol to be paste, and drying the paste in vacuum to obtain the broccoli leftover extractive. The preparation method disclosed by the invention has the characteristics that a complex enzyme preparation, ultrasonic-assisted extraction and microwave-assisted extraction are applied to the extraction of effective components in broccoli leftovers; and compared with the prior art, the preparation method improves the additional value of the broccoli leftovers greatly and the yield of the raphanin and flavonoid compounds, and also has the characteristics of low energy consumption, moderate extraction condition and low cost. The broccoli leftover extractive can be widely applied to the fields of bulk pharmaceutical chemicals, biochemical engineering and health-care foods, and has wide market prospect.

Description

technical field [0001] The invention relates to a process for extracting sulforaphane and flavonoids from leftovers produced by quick-frozen broccoli. Background technique [0002] Broccoli scientific name Brassica oleracea L. var. botrytis L. , is an annual plant of the genus Brassicaceae and Brassica. Broccoli is a functional vegetable. In addition to being rich in vitamin C and other nutrients, it also has anti-cancer, anti-cancer, scavenging free radicals in the body, anti-oxidation, and anti-oxidation. It has many health benefits such as aging, and it ranks fourth among the top ten healthy foods recommended by Time magazine. [0003] In addition to being eaten directly, broccoli is mostly processed into quick-frozen broccoli. The total amount of broccoli used to process quick-frozen broccoli in my country reaches more than 1 million tons every year. There are about 20%, resulting in a large amount of broccoli scrap resources. In the past, broccoli scraps were mainly u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61P35/00A61P39/06A23L1/29A23L33/00
Inventor 万志祥万上
Owner 湘西新金凤凰农业开发有限公司
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