Method for measuring paeoniflorin, hesperidin and glycyrrhizic acid in stomach-nourishing granules

A technology of paeoniflorin and hesperidin, which is applied in the field of content determination of traditional Chinese medicine preparations, can solve problems such as difficulty in reflecting the quality of medicines, and achieve the effects of saving time and testing costs, effectively controlling quality, and improving quality control standards.

Inactive Publication Date: 2013-06-26
CHIATAI QINGCHUNBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Yangwei Granules only measured paeoniflorin in Radix Paeoniae Alba under the content determination item in the 2010 edition of Ph

Method used

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  • Method for measuring paeoniflorin, hesperidin and glycyrrhizic acid in stomach-nourishing granules
  • Method for measuring paeoniflorin, hesperidin and glycyrrhizic acid in stomach-nourishing granules
  • Method for measuring paeoniflorin, hesperidin and glycyrrhizic acid in stomach-nourishing granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Determination of the content of paeoniflorin, hesperidin and glycyrrhizic acid in Yangwei granules by high performance liquid chromatography

[0039] (1) High performance liquid chromatography conditions

[0040] Instrument name: Agilent 1100 High Performance Liquid Chromatograph

[0041] Chromatographic conditions: Chromatographic column: ZORBAX SB—C18 (column length 250mm, inner diameter 4.6mm), filler: octadecylsilane bonded silica gel (particle size 5μm), flow rate: 1.0ml / min, column temperature: 25℃

[0042] DAD UV detector detection wavelength: 230nm, 283nm, 252nm

[0043] Use 0.5% glacial acetic acid aqueous solution as mobile phase A and acetonitrile as mobile phase B, and perform gradient elution according to Table 1.

[0044] Table 1 Gradient elution conditions

[0045] Time (minutes)

Mobile phase A (%)

Mobile phase B (%)

0~10

85

15

10~30

85→80

15→20

30~65

80→50

20→50

[0046] (2) Preparation of standard solution:

[0047] a. Paeoniflorin standard ...

Embodiment 2

[0061] Accurately weigh the standard paeoniflorin and add 50% methanol aqueous solution with volume concentration to make a standard solution containing 220μg paeoniflorin per 1ml.

[0062] Accurately weigh the standard hesperidin and add 50% methanol aqueous solution with volume concentration to make a standard solution of 14μg per 1ml hesperidin.

[0063] Accurately weigh the standard ammonium glycyrrhizinate and add a 50% methanol aqueous solution with a volume concentration to make a standard solution containing 40 μg ammonium glycyrrhizinate per 1 ml. (Glycyrrhizic acid weight = ammonium glycyrrhizinate weight / 1.0207).

[0064] The 50% ethanol aqueous solution with volume concentration in step (3) in Example 1 was replaced with 50% methanol aqueous solution with volume concentration. The other operations were the same as in Example 1. The measurement result: the content of paeoniflorin in each bag (5g) of Yangwei granules was 24.40mg , The content of hesperidin is 0.81mg, and...

Embodiment 3

[0066] Accurately weigh the standard amount of paeoniflorin and add a 70% methanol aqueous solution with volume concentration to make a standard solution containing 180μg of paeoniflorin per 1ml.

[0067] Accurately weigh the standard hesperidin and add a 70% methanol aqueous solution with a volume concentration to make a standard solution of 10 μg per 1 ml of hesperidin.

[0068] Accurately weigh the standard ammonium glycyrrhizinate, add a volume concentration of 70% methanol aqueous solution to prepare a standard solution containing 42 μg of ammonium glycyrrhizinate per 1 ml. (Glycyrrhizic acid weight = ammonium glycyrrhizinate weight / 1.0207).

[0069] The 50% ethanol aqueous solution with volume concentration in step (3) in Example 1 was replaced with 70% methanol aqueous solution, and ultrasonic treatment was performed for 20 minutes. The other operations were the same as in Example 1. The measurement result: Paeonia lactiflora in each bag (5g) of Yangwei granules The glycosi...

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Abstract

The invention discloses a method for measuring paeoniflorin, hesperidin and glycyrrhizic acid in stomach-nourishing granules. The method comprises the following steps: pretreating the stomach-nourishing granules into a sample to be measured, injecting the sample to be measured into a high performance liquid chromatograph for testing to obtain a high performance liquid chromatography spectrogram of the sample to be measured, and calculating the contents of paeoniflorin, hesperidin and glycyrrhizic acid in the sample to be measured according to the peak area of the corresponding absorption peak in the high performance liquid chromatography spectrogram of the sample to be measured and the standard curve of each standard substance. According to the method, the components of paeoniflorin under the stomach-nourishing granule content determination item in version 1 in pharmacopoeia of the people's republic of China in 2010 are measured, the content of hesperidin in dried tangerine or orange peel and glycyrrhizic acid content in liquorice are measured, the quality control standard of the stomach-nourishing granules is greatly improved, the content determination methodology validation of the method completely accords with the provision, and the method is an advanced quality control method; and moreover, the time and detection cost can be saved, and the product quality can be effectively controlled.

Description

(1) Technical field [0001] The invention relates to a method for determining the content of traditional Chinese medicine preparations, in particular to a method for determining the content of paeoniflorin, hesperidin and glycyrrhizic acid in the medicine. (2) Background technology [0002] The prescription composition of Yangwei Granules is 500g of Sunflower Astragalus, 333g of Codonopsis, 500g of Radix Paeoniae Alba, 281g of Licorice, 250g of Tangerine Peel, 500g of Cyperus rotundus, 167g of Wumei, and 500g of Chinese Yam. The preparation method is to extract the volatile oil from tangerine peel and use the medicine residue for later use; add water to decoct the other seven herbs twice, the first time is 2 hours, the second time is 1 hour, the second time, the above-mentioned tangerine peel medicine residue is added, filtered, and the filtrate is combined , Set aside, take the supernatant and concentrate it to a clear ointment with a relative density of 1.16~1.19 (20℃), add 446....

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 林徐剑吴碧元柴建国施晓萍沈莹
Owner CHIATAI QINGCHUNBAO PHARMA
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