Method for rapidly detecting melamine in forage based on surface enhanced Raman scattering spectrum

Abstract

The invention discloses a method for rapidly detecting melamine in forage based on a surface enhanced Raman scattering spectrum. The method comprises the following steps of: carrying out liquid-liquid extraction prurification on an extracting solution, wherein normal butanol or ethyl acetate is taken as an extraction solvent, and an acid water solution and an organic solvent are taken as purification extracting solvents; then, adding the purified solution to be detected and a saline solution containing Cl<-> to a nanogold colloidal solution or a nano-silver colloidal solution to obtain a mixed solution which is a final loaded detection solution; carrying out spectrum scanning on the detection reagent by the 785nm laser source Raman spectrum, wherein 707+ / -4 cm<-1> is taken as qualitative feature peak displacement of the melamine; and carrying out normalization by taking 731+ / -4 cm<-1> as corresponding peak intensity, and calculating the melamine content by taking 707+ / -4 cm<-1> as corresponding peak intensity. The method provided by the invention can rapidly detect the melamine in the forage in situ.

Description

Technical field [0001] The invention relates to a method for detecting melamine in feed, in particular to a method for rapidly detecting melamine in feed based on surface enhanced scattering Raman spectrum. Background technique [0002] Melamine is abbreviated as triamine, scientific name triamine triazine, commonly known as melamine, protein essence, is an important nitrogen heterocyclic organic chemical raw material, mainly used to produce melamine-formaldehyde resin, used in wood processing, plastics and other industries , Because each melamine molecule contains 6 nitrogen atoms, in the original Kjeldahl method of crude protein content, the protein content is determined by the nitrogen content, which brings opportunities for criminals , By adding cheap industrial raw materials with high nitrogen content to increase its nitrogen content, resulting in a false increase in protein content. In March 2007, a number of deaths from cats and dogs pet poisoning occurred in the United S...

AI Technical Summary

Problems solved by technology

At present, the agricultural industry standard issued by the Ministry of Agriculture of China - Determination of Melamine in Feed (NY / T1372-2007) is widely used. This standard method uses HPLC method and GC-MS method, and GC-MS method is used as the confirmation method These two methods have long analysis time, cumbersome pretreatment, and GC-MS requires derivatization operations, which are not suitable for rapid on-site detection; although the existing ELISA rapid screening method is relatively simple, it is limited by environmental conditions and often appears " "false positive" situation brings inconvenience to the detection work; the use of near-infrared to detect melamine is limited by the near-infrared method itself and cannot meet the regulatory requirements at the ppm level

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