Method for rapidly detecting melamine in forage based on surface enhanced Raman scattering spectrum

A technology of melamine and Raman spectroscopy, applied in the field of detection of melamine in feed, can solve the problems of false positives, inconvenient detection work, long analysis time, etc., and achieve the effect of rapid detection

Active Publication Date: 2013-07-10
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the agricultural industry standard issued by the Ministry of Agriculture of China - Determination of Melamine in Feed (NY/T1372-2007) is widely used. This standard method uses HPLC method and GC-MS method, and GC-MS method is used as the confirmation method These two methods have long analysis time, cumbersome pretreatment, and GC-MS requires derivatization operations, which are no

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly detecting melamine in forage based on surface enhanced Raman scattering spectrum
  • Method for rapidly detecting melamine in forage based on surface enhanced Raman scattering spectrum
  • Method for rapidly detecting melamine in forage based on surface enhanced Raman scattering spectrum

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0041] The specific embodiment includes the steps:

[0042] (1) Sample pretreatment:

[0043] Method 1: Accurately weigh 1.0g feed, add 5mL n-butanol, vortex extraction for 30s, centrifuge at 12500rpm for 1min, remove 3mL supernatant, add 3mL pH=3-4 HCl aqueous solution, vortex centrifugation, remove the lower layer Add 10μL of 1M NaOH solution, 500μL of ethyl acetate: n-butanol mixture (V / V=6:1), vortex and centrifuge, pipette the upper layer into 500μL of pH=3-4HCl aqueous solution, vortex , The lower liquid is the liquid to be tested.

[0044] Method 2: Accurately weigh 1.0g of feed, add 5mL ethyl acetate, vortex and shake for 30s, centrifuge at 12500rpm, pipette 3mL of supernatant, add 3mL pH=3-4 HCl aqueous solution, vortex centrifugation, remove the lower layer 500μL, add 10μL of 1M NaOH solution, 500μL of ethyl acetate, vortex and centrifuge, pipette the upper layer and add 500μL of pH=3-4 HCl aqueous solution, vortex and centrifuge, the lower layer is the test solution.

[0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for rapidly detecting melamine in forage based on a surface enhanced Raman scattering spectrum. The method comprises the following steps of: carrying out liquid-liquid extraction prurification on an extracting solution, wherein normal butanol or ethyl acetate is taken as an extraction solvent, and an acid water solution and an organic solvent are taken as purification extracting solvents; then, adding the purified solution to be detected and a saline solution containing Cl<-> to a nanogold colloidal solution or a nano-silver colloidal solution to obtain a mixed solution which is a final loaded detection solution; carrying out spectrum scanning on the detection reagent by the 785nm laser source Raman spectrum, wherein 707+/-4 cm<-1> is taken as qualitative feature peak displacement of the melamine; and carrying out normalization by taking 731+/-4 cm<-1> as corresponding peak intensity, and calculating the melamine content by taking 707+/-4 cm<-1> as corresponding peak intensity. The method provided by the invention can rapidly detect the melamine in the forage in situ.

Description

Technical field [0001] The invention relates to a method for detecting melamine in feed, in particular to a method for rapidly detecting melamine in feed based on surface enhanced scattering Raman spectrum. Background technique [0002] Melamine is abbreviated as triamine, scientific name triamine triazine, commonly known as melamine, protein essence, is an important nitrogen heterocyclic organic chemical raw material, mainly used to produce melamine-formaldehyde resin, used in wood processing, plastics and other industries , Because each melamine molecule contains 6 nitrogen atoms, in the original Kjeldahl method of crude protein content, the protein content is determined by the nitrogen content, which brings opportunities for criminals , By adding cheap industrial raw materials with high nitrogen content to increase its nitrogen content, resulting in a false increase in protein content. In March 2007, a number of deaths from cats and dogs pet poisoning occurred in the United S...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/65G01N1/34
Inventor 程劼王石苏晓鸥
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap