Novel synthesis method for multilayer protection hyperstable water-soluble single fluorescent quantum dot and fluorescent microsphere

A technology of fluorescent quantum dots and fluorescent microspheres, which is applied in the intersection of nanomaterial technology and biotechnology, can solve the problems of decreased biological detection sensitivity, loss of detection of ractopamine antigen, and inability to detect fluorescent signals by chromatography test strips. Effects of improved stability, improved stability and sensitivity

Inactive Publication Date: 2013-07-17
HENAN UNIVERSITY
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Problems solved by technology

In this case, the sensitivity of the bioassay will be greatly reduced. In extreme cases, although the result is positive, no fluorescent signal can be detected on the chromatography test strip.
For example, polymer-modified CdSe / ZnS quantum dots were used to detect ractopamine (Rac) residues in pig urine due to its high concentration of NH 4 + , K + , HCO 3 - , Cl - As well as a strong acidic environment, quantum dots inevitably suffer from an obvious "fluorescence quenching" phenomenon, thus losing the ability to detect ractopamine antigens

Method used

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  • Novel synthesis method for multilayer protection hyperstable water-soluble single fluorescent quantum dot and fluorescent microsphere
  • Novel synthesis method for multilayer protection hyperstable water-soluble single fluorescent quantum dot and fluorescent microsphere
  • Novel synthesis method for multilayer protection hyperstable water-soluble single fluorescent quantum dot and fluorescent microsphere

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Experimental program
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Effect test

Embodiment 1

[0035] Preparation of water-soluble single CdSe / ZnS fluorescent quantum dots (PL = 624 nm) modified by amphoteric oligomers: Dissolve 0.2 g of amphoteric oligomer-polycetyl maleate (PMAH) in 10 mL of chloroform , followed by adding 2 mL of CdSe / ZnS (0.04 g, dissolved in chloroform) oil-soluble quantum dots and stirring to mix. After the mixing is completed, the chloroform is volatilized by heating, and the volatilization is complete within 2 minutes. Then add 10 mL ammonia solution (containing 1 mL concentrated ammonia water, 30%), and sonicate for 20 minutes until the quantum dots are completely dissolved in water. Finally, the quantum dot aqueous solution was filtered through a 0.2-μm microporous membrane. After the filtration, a clear quantum dot aqueous solution was obtained. Centrifuge at 20,000 rpm for 30 min to remove excess polymer, repeat the centrifugation process three times, and redisperse in 5 mL of deionized water for later use. Oil-soluble (a) and water-solub...

Embodiment 2

[0041] Preparation of water-soluble single CdSe / ZnS fluorescent quantum dots (PL=624 nm): same as Example 1.

[0042] Preparation of silica-CdSe / ZnS composite particles: Add the above-mentioned quantum dot aqueous solution into 80 mL of absolute ethanol, stir for 1 h and add an appropriate amount of ammonia dropwise to adjust the pH value of the solution between 8-10. In the reaction system, 20 uL of tetraethyl orthosilicate was added and reacted at 25 oC for 12 h. After the reaction, centrifuge three times and redisperse in 20 mL of absolute ethanol. Characterized by TEM, its particle size is 16.1 nm ( Figure 9 shown).

[0043] The preparation of oil-soluble silica-CdSe / ZnS composite particle: same as embodiment 1.

[0044] Preparation of multilayer protected CdSe / ZnS composite particles: the process of preparing water-soluble silica-CdSe / ZnS composite particles is similar to that of water-soluble CdSe / ZnS fluorescent quantum dots, except that the amount of polycetyl male...

Embodiment 3

[0046] Preparation of amphoteric oligomer-modified water-soluble CdSe / ZnS fluorescent microspheres (PL = 624 nm): 2 mL of CdSe / ZnS (0.04 g, dissolved in chloroform) oil-soluble quantum dots were dissolved in 10 mL of chloroform, and then Add a certain quality of amphoteric oligomer - poly cetyl maleate (PMAH) and stir to mix. After mixing (the molar ratio of PMAH and quantum dots can be adjusted in the range of 20:1-120:1), heat and volatilize the chloroform, and the volatilization is complete within 2 minutes. Then add 10 mL ammonia solution (containing 1 mL concentrated ammonia water, 30%), and sonicate for 20 minutes until the fluorescent microspheres are completely dissolved in water. Finally, the fluorescent microsphere aqueous solution was filtered through a 0.45-μm microporous membrane. Centrifuge at 10,000 rpm for 20 min to remove excess polymer, repeat the centrifugation process three times, and redisperse in 5 mL of deionized water for later use. The TEM photograph...

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Abstract

The invention relates to a novel synthesis method for a multilayer protection hyperstable water-soluble single fluorescent quantum dot and a fluorescent microsphere. The method comprises the following steps: transferring an oil-soluble fluorescent quantum dot to a water phase by using an amphoteric oligomer; then wrapping a water-soluble single fluorescent quantum dot and a fluorescent microsphere in silica; allowing the surfaces of the single fluorescent quantum dot and the fluorescent microsphere wrapped by silica to carry hydrophobic groups; and finally preparing the multilayer protection hyperstable water-soluble single fluorescent quantum dot and the fluorescent microsphere by using the amphoteric oligomer again. With the method, the fluorescent quantum dot can be protected from a destructive effect of an external environment (e.g., a strong acid, a strong alkali, a salt, etc.) to a greatest extent. The multilayer protection hyperstable water-soluble single fluorescent quantum dot and the fluorescent microsphere can be used in a microporous immunostrip biological detection system for detection of human chorionic gonadotropin (HCG), human immunodeficiency virus (HIV), hepatitis and clenbuterol--ractopamine(Rac) residual and the like in swine urine.

Description

technical field [0001] The invention belongs to the interdisciplinary field of nanomaterial technology and biotechnology, and relates to a new method for synthesizing ultra-stable water-soluble single fluorescent quantum dots and fluorescent microspheres with multi-layer protection. This method can synthesize single fluorescent quantum dots and fluorescent microspheres with different functions in different preparation stages: water-soluble single fluorescent quantum dots and fluorescent microspheres modified by amphiphilic oligomers, oil-soluble single fluorescent quantum dots wrapped in silica and Fluorescent microspheres and multi-layer protected water-soluble single fluorescent quantum dots and fluorescent microspheres. The multi-layer protected single fluorescent quantum dot and fluorescent microsphere synthesized by the method have greatly improved fluorescence stability compared with the traditional method, and can be used for cell labeling, solid-state light-emitting de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/02
Inventor 李林松周长华申怀彬
Owner HENAN UNIVERSITY
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