Preparation method of anti-oxidation water-in-oil microemulsion
A technology of microemulsion and oxidized oil, which is applied in emulsion delivery, cosmetic preparations, pharmaceutical formulations, etc. It can solve the problems of alteration, teratogenicity, and carcinogenicity, and achieve the effects of increasing added value, improving quality, and extending shelf life
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Embodiment 1
[0027] 1) Preparation of peach kernel protein isolate:
[0028] 1.1) Soak peach kernels and peel them, dry and crush them at 35°C, degrease them with petroleum ether for 4 times, pass through a 60-mesh sieve after evaporating the solvent, and dry them at low temperature to get peach kernel degreasing powder. Add 1g of peeled peach kernels each time 6mL of petroleum ether;
[0029] 1.2) Mix peach kernel defatted powder and distilled water at a mass ratio of 1:10, adjust the pH value to 8.0 with 0.5mol / L NaOH solution, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20 minutes, collect the supernatant, and mix the obtained precipitate with distilled water Mix at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, collect the supernatant, mix the obtained precipitate with distilled water at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, and collect t...
Embodiment 2
[0040] 1) Preparation of peach kernel protein isolate:
[0041] 1.1) Soak peach kernels and peel them, dry and crush them at 35°C, degrease them with petroleum ether for 4 times, pass through a 60-mesh sieve after evaporating the solvent, and dry them at low temperature to get peach kernel degreasing powder. Add 1g of peeled peach kernels each time 6mL of petroleum ether;
[0042] 1.2) Mix peach kernel defatted powder and distilled water at a mass ratio of 1:10, adjust the pH value to 8.0 with 0.5mol / L NaOH solution, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20 minutes, collect the supernatant, and mix the obtained precipitate with distilled water Mix at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, collect the supernatant, mix the resulting precipitate with distilled water at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, and collect ...
Embodiment 3
[0052] 1) Preparation of peach kernel protein isolate:
[0053] 1.1) Soak peach kernels and peel them, dry and crush them at 35°C, degrease them with petroleum ether for 4 times, pass through a 60-mesh sieve after evaporating the solvent, and dry them at low temperature to get peach kernel degreasing powder. Add 1g of peeled peach kernels each time 6mL petroleum ether;
[0054] 1.2) Mix peach kernel defatted powder and distilled water at a mass ratio of 1:10, adjust the pH value to 8.0 with 0.5mol / L NaOH solution, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20 minutes, collect the supernatant, and mix the obtained precipitate with distilled water Mix at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, collect the supernatant, mix the obtained precipitate with distilled water at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, and collect the ...
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