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Circulating synchronous straw enzymatic hydrolysis fermentation fuel ethanol reactor and reaction method thereof

A circulating reactor and synchronous enzymatic hydrolysis technology, which is applied in the direction of bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, fermentation, etc., to achieve the effect of reducing dosage, saving production cost and improving production efficiency

Active Publication Date: 2013-08-14
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned problems existing in the reactor in the synchronous enzymolysis and fermentation process in the prior art, in order to solve the problem of sufficient recycling of the mixed bacterial solution rich in cellulase and yeast, the present invention provides a circulation formula with simple process and remarkable effect. Integrated reactor

Method used

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  • Circulating synchronous straw enzymatic hydrolysis fermentation fuel ethanol reactor and reaction method thereof

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Effect test

Embodiment 1

[0043] (1) Pulverization: After cleaning the fresh sorghum straw, cut it with a shredding machine or pulverize it with a hammer mill, sieve it into 40mm straw powder, and control the moisture content below 55%.

[0044] (2) Biological pretreatment: sorghum straw powder is treated by white rot fungi for lignin degradation.

[0045] (3) Sterilization: put the sorghum straw powder into the circulating integrated reactor for high temperature sterilization, 121 ℃, 30min sterilization treatment.

[0046] (4) Double-enzyme saccharification and fermentation: The mixed solution of cellulase, xylanase and Saccharomyces cerevisiae is sprayed downward from the top of the fermenter, and the amount of cellulase whose activity is 1000IU / g is 1.5% of the weight of the straw. , the amount of Saccharomyces cerevisiae with an activity of 6 billion / g is 1% of the weight of the straw, the amount of water is 2.5 times the weight of the straw powder, the enzymatic hydrolysis and fermentation temper...

Embodiment 2

[0053] (1) Pulverization: After cleaning the fresh corn stalks, cut them with a shredding machine or pulverize them with a hammer mill, sieve them into 30mm straw powder, and control the moisture content at 50%.

[0054] (2) Biological pretreatment: corn stalk powder is subjected to lignin degradation treatment by white rot fungi.

[0055] (3) Sterilization: put the corn stalk powder into the circulating integrated reactor for high temperature sterilization, 121 ℃, 30min sterilization treatment.

[0056] (4) Double-enzyme saccharification and fermentation: the mixed solution of cellulase, xylanase and Saccharomyces cerevisiae is sprayed downward from the top of the fermenter, and the addition amount of cellulase with an activity of 1000 IU / g is 2% of the weight of the straw , the amount of Saccharomyces cerevisiae with an activity of 8 billion / g is 0.8% of the weight of the straw, the amount of water is 2.5 times the weight of the straw powder, the enzymatic hydrolysis and fer...

Embodiment 3

[0063] (1) Pulverization: After cleaning the fresh rice straw, cut it with a shredding machine or pulverize it with a hammer mill, sieve it into 30mm straw powder, and control the moisture content at 53%.

[0064] (2) Biological pretreatment: The rice straw powder is subjected to lignin degradation treatment by white rot fungi.

[0065] (3) Sterilization: The rice straw powder was put into a circulating integrated reactor for high temperature sterilization, 121℃, 30min sterilization treatment.

[0066] (4) Double-enzyme saccharification and fermentation: the mixed solution of cellulase, xylanase and Saccharomyces cerevisiae is sprayed downward from the top of the fermenter, and the addition amount of cellulase with an activity of 1000 IU / g is 2% of the weight of the straw , the amount of Saccharomyces cerevisiae with an activity of 8 billion / g is 1% of the weight of the straw, the amount of water is 2 times the weight of the straw powder, the enzymatic hydrolysis and fermenta...

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Abstract

The invention discloses a circulating synchronous straw enzymatic hydrolysis fermentation fuel ethanol reactor and a reaction method thereof. The reactor comprises a mechanical stirring fermentation tank, an air purification device, a steam generation device, a temperature control system, a distillation tower, a lower computer control system, an upper computer monitoring system, a speed regulator, a stirring motor, and a pressure pump, as well as a ceramic ultrafiltration membrane separation device, wherein the upper end of the ceramic ultrafiltration membrane separation device is connected with a return port at the upper end of the mechanical stirring fermentation tank, and the lower end of the ceramic ultrafiltration membrane separation device is connected with a fermentation residue discharge outlet at the lower end of the mechanical stirring fermentation tank through the pressure pump. The reactor integrates sterilization, enzymatic hydrolysis and fermentation, thus not only being used for production of synchronous straw enzymatic hydrolysis fermentation fuel ethanol, but also being used for production of fuel ethanol through step-by-step straw enzymatic hydrolysis and fermentation technique; the recycling of cellulose and yeast can be realized by adopting the ceramic ultrafiltration membrane separation device; and the reactor not only can be used for production of the straw enzymatic hydrolysis fermentation fuel ethanol, but also can be used for fermentation production of antibiotics, amino acids, enzymes and other biological products.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation equipment, and relates to an integrated reactor that uses straw as raw material and adopts a synchronous enzymatic hydrolysis fermentation process, which can reuse the fermentation liquid containing cellulase, xylanase and yeast to produce fuel ethanol and its reaction method. Background technique [0002] Using renewable straw waste as raw material and using microbial fermentation technology to produce fuel ethanol has become an important strategic measure to alleviate my country's energy crisis, comprehensively utilize straw waste, and ensure energy security. However, large-scale and industrialized production has not yet been formed. The main reason is that the production cost is too high. The process of producing ethanol from cellulose in straw, represented by corn stalk, generally includes three steps of pretreatment, enzymatic hydrolysis and fermentation. Among them, enzymati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/12C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 黄永红孙玉坤章金元张志才王博
Owner JIANGSU UNIV
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