Circulating synchronous straw enzymatic hydrolysis fermentation fuel ethanol reactor and reaction method thereof
A circulating reactor and synchronous enzymatic hydrolysis technology, which is applied in the direction of bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, fermentation, etc., to achieve the effect of reducing dosage, saving production cost and improving production efficiency
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Embodiment 1
[0043] (1) Pulverization: After cleaning the fresh sorghum straw, cut it with a shredding machine or pulverize it with a hammer mill, sieve it into 40mm straw powder, and control the moisture content below 55%.
[0044] (2) Biological pretreatment: sorghum straw powder is treated by white rot fungi for lignin degradation.
[0045] (3) Sterilization: put the sorghum straw powder into the circulating integrated reactor for high temperature sterilization, 121 ℃, 30min sterilization treatment.
[0046] (4) Double-enzyme saccharification and fermentation: The mixed solution of cellulase, xylanase and Saccharomyces cerevisiae is sprayed downward from the top of the fermenter, and the amount of cellulase whose activity is 1000IU / g is 1.5% of the weight of the straw. , the amount of Saccharomyces cerevisiae with an activity of 6 billion / g is 1% of the weight of the straw, the amount of water is 2.5 times the weight of the straw powder, the enzymatic hydrolysis and fermentation temper...
Embodiment 2
[0053] (1) Pulverization: After cleaning the fresh corn stalks, cut them with a shredding machine or pulverize them with a hammer mill, sieve them into 30mm straw powder, and control the moisture content at 50%.
[0054] (2) Biological pretreatment: corn stalk powder is subjected to lignin degradation treatment by white rot fungi.
[0055] (3) Sterilization: put the corn stalk powder into the circulating integrated reactor for high temperature sterilization, 121 ℃, 30min sterilization treatment.
[0056] (4) Double-enzyme saccharification and fermentation: the mixed solution of cellulase, xylanase and Saccharomyces cerevisiae is sprayed downward from the top of the fermenter, and the addition amount of cellulase with an activity of 1000 IU / g is 2% of the weight of the straw , the amount of Saccharomyces cerevisiae with an activity of 8 billion / g is 0.8% of the weight of the straw, the amount of water is 2.5 times the weight of the straw powder, the enzymatic hydrolysis and fer...
Embodiment 3
[0063] (1) Pulverization: After cleaning the fresh rice straw, cut it with a shredding machine or pulverize it with a hammer mill, sieve it into 30mm straw powder, and control the moisture content at 53%.
[0064] (2) Biological pretreatment: The rice straw powder is subjected to lignin degradation treatment by white rot fungi.
[0065] (3) Sterilization: The rice straw powder was put into a circulating integrated reactor for high temperature sterilization, 121℃, 30min sterilization treatment.
[0066] (4) Double-enzyme saccharification and fermentation: the mixed solution of cellulase, xylanase and Saccharomyces cerevisiae is sprayed downward from the top of the fermenter, and the addition amount of cellulase with an activity of 1000 IU / g is 2% of the weight of the straw , the amount of Saccharomyces cerevisiae with an activity of 8 billion / g is 1% of the weight of the straw, the amount of water is 2 times the weight of the straw powder, the enzymatic hydrolysis and fermenta...
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