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Method for separating berberine hydrochloride by applying berberine hydrochloride molecularly imprinted chromatographic column

A technology of berberine hydrochloride and molecular imprinting, applied in the field of high performance liquid chromatography, to achieve the effects of good stability, simple operation, and small expansion

Active Publication Date: 2015-11-04
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] There is no report about the application of berberine hydrochloride molecularly imprinted chromatography column to separate berberine hydrochloride

Method used

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  • Method for separating berberine hydrochloride by applying berberine hydrochloride molecularly imprinted chromatographic column
  • Method for separating berberine hydrochloride by applying berberine hydrochloride molecularly imprinted chromatographic column
  • Method for separating berberine hydrochloride by applying berberine hydrochloride molecularly imprinted chromatographic column

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Add 0.034g of berberine hydrochloride and 0.25g of methacrylic acid into a 50mL beaker in turn, after the template molecules and berberine hydrochloride are mixed evenly, then add 12g of maleic rosin ethylene glycol acrylate and 2.4g of dimethic acid Ethylene glycol methacrylate, 3g peanut oil, 20g ethyl acetate, and 0.01g azobisisobutyronitrile were solubilized by ultrasonic waves to form a uniform oil phase.

[0037] Add 76g of deionized water, 0.0836g of sodium lauryl sulfate, and 0.0669g of polyvinyl alcohol to a 1000mL three-neck flask in sequence, adjust the stirring speed to 600r / min, heat to promote the complete dissolution of sodium lauryl sulfate and polyvinyl alcohol, and wait until After the temperature of the water phase rose to 55°C, the oil phase was slowly added into the three-necked flask, pre-dispersed for 10 minutes to make the oil phase form uniform small droplets in the water phase, and slowly heated to 85°C to initiate polymerization by free rad...

Embodiment 2

[0040] (1) Put 3.4g of berberine hydrochloride and 25g of methacrylic acid in a 500mL beaker, after the template molecules and berberine hydrochloride are mixed evenly, then add 12g of maleic rosin ethylene glycol acrylate and 36g of dimethacrylic acid Ethylene glycol ester, 20g peanut oil, 100g ethyl acetate, 0.7g azobisisobutyronitrile, solubilized by ultrasonic to form a uniform oil phase.

[0041] Add 1770g of deionized water, 0.3894g of sodium lauryl sulfate, and 0.1947g of polyvinyl alcohol to a 3000mL three-neck flask in sequence, adjust the stirring speed to 200r / min, and heat to promote the complete dissolution of sodium lauryl sulfate and polyvinyl alcohol. After the temperature of the water phase rose to 80°C, the oil phase was slowly added into the three-necked flask, pre-dispersed for 10 minutes to make the oil phase form uniform small droplets in the water phase, and slowly heated to 95°C to initiate free radical polymerization for 4 hours.

[0042] (2) After the...

Embodiment 3

[0044] (1) Add 0.7442g berberine hydrochloride and 5g methacrylic acid into a 150mL beaker, after the template molecules and berberine hydrochloride are evenly mixed, then add 30g maleic rosin ethylene glycol acrylate and 10g dimethacrylic acid Ethylene glycol ester, 20g peanut oil, 120g ethyl acetate, 0.7875g azobisisobutyronitrile, solubilized by ultrasonic to form a uniform oil phase.

[0045] Add 600g of deionized water, 0.5325g of sodium lauryl sulfate, and 0.2663g of polyvinyl alcohol to a 2000mL three-neck flask in sequence, adjust the stirring speed to 500r / min, and heat to completely dissolve the sodium lauryl sulfate and polyvinyl alcohol. After the temperature of the water phase rose to 65°C, the oil phase was slowly added into the three-necked flask, pre-dispersed for 10 minutes to make the oil phase form uniform small droplets in the water phase, and slowly heated to 85°C to initiate polymerization by free radicals for 3 hours.

[0046] (2) After the reaction is f...

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Abstract

The invention discloses a method for separating berberine hydrochloride by applying a berberine hydrochloride molecular imprinting chromatographic column. The method comprises the following steps of (1) preparing a sample solution, namely taking moderate sample and dissolving into an organic solvent so as to prepare a sample solution containing 1.0*10<-5>mol-3.0*10<-4>mol of berberine hydrochloride per liter, wherein the sample is the mixture of berberine hydrochloride and ligustrazine or the mixture of the berberine hydrochloride and tetrahydropalmatine; (2) setting parameters, namely inserting the berberine hydrochloride molecular imprinting chromatographic column into a liquid chromatograph, and setting the flowing speed of a moving phase of the liquid chromatograph to be 0.1-1mL / min, the detected wavelength to be 345nm, and a column oven to be at 30+ / -10 DEG C; and (3) separating, namely starting an injection valve and bringing the sample solution into the berberine hydrochloride molecular imprinting chromatographic column by using the moving phase, so as to separate the berberine hydrochloride. The method has high sensitivity, is simple to operate, and has no secondary contamination to medicaments and health care products.

Description

technical field [0001] The invention belongs to the field of high performance liquid chromatography, in particular to a method for separating berberine hydrochloride by using a molecular imprinted chromatographic column of berberine hydrochloride. Background technique [0002] Berberine hydrochloride, chemical structural formula: [0003] , is the hydrochloride of berberine, the main component of natural medicine Coptis chinensis. It not only has the effects of clearing away heat and dampness, purging fire and detoxifying, but also has pharmacological activities such as antibacterial, hypoglycemic, antitumor and antiarrhythmic. It is effective for tuberculosis, scarlet fever, Acute tonsillitis and respiratory tract infection also have certain curative effect. [0004] Molecular imprinting technology uses the separated and detected substance as a template molecule, immobilizes it in a polymer with a high degree of cross-linking, and after removing the template molecule, le...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D455/03B01D15/08
Inventor 雷福厚王婷李鹏飞刘祖广申利群姚兴东周菊英
Owner GUANGXI UNIV FOR NATITIES