Novel sea asparagus triterpenoid saponin reducing compound as well as preparation method and application thereof
A technology of triterpene saponins and compounds, applied in the field of new compounds of sea asparagus nortriterpene saponins and their preparation
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Embodiment 1
[0020] The preparation of embodiment 1 compound
[0021] 22.6 kg of freshly picked sea asparagus aerial parts were crushed with a homogenizer, placed in a 20 L volumetric flask, soaked in 10 L of 80% acetone aqueous solution, and left to extract for 24 h; the extract was filtered through a Buchner funnel , the filtered sea asparagus residue was repeatedly extracted twice with 10 L of 80% acetone aqueous solution, and the extract was concentrated under reduced pressure to contain no acetone; it was extracted three times with an equal volume of ethyl acetate to remove most of the fat-soluble components, and the raffinate Extract with an equal volume of methanol 3 times, filter, take the upper layer, concentrate to dryness, and obtain methanol extract.
[0022] The methanol extract (85 g) was mixed with 60 g of silica gel in a dry method, and then mounted on an atmospheric column equipped with 200 g of silica gel (300-400 mesh), followed by petroleum ether-acetone (50:1, 20:1, 10...
Embodiment 2
[0032] Example 2 Test of antitumor activity
[0033] 1. Experimental method and reagent preparation
[0034] MTT method was used to determine the inhibitory effect of two compounds on A549 and hepG2 cells: take A549 (human lung cancer cells) and hepG2 (human liver cancer cells) in logarithmic growth phase, adjust the concentration of cell suspension, and add 100 μL per well to 96-well cells In the culture plate, plate to make the cell density to be tested 3000 / well, and in 5% CO 2 , in an incubator at 37°C for 4 h; 5 concentration gradients of 0.1, 1, 10, 25, and 50 μM were set for the samples to be tested, and 5 parallels were set for each concentration. Add 100 μL of sample solution or blank solution (cells, drug dissolution medium, and cell culture medium) to each well, culture for 72 h, add 20 μL of MTT solution (5 mg / mL, prepared in PBS solution) to each well, and continue to culture for 4 h. Centrifuge at 2,000 rpm for 8 minutes at 37°C, and remove the supernatant with...
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