Spectinomycin high-producing strain as well as preparation method and application thereof

A technology of spectinomycin and high-yield strains, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of low fermentation yield of strains, increase abundance, increase fermentation yield, and enhance inducement Variation effect

Active Publication Date: 2013-09-25
河北圣雪大成唐山制药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the method has screened out strains with good resistance to multiple d

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  • Spectinomycin high-producing strain as well as preparation method and application thereof
  • Spectinomycin high-producing strain as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of spectinomycin high-yield strains

[0026] (1) Preparation of spore suspension: the starting strain (No. 4.467, purchased from the Institute of Microbiology, Chinese Academy of Sciences) was inoculated on the slant medium, and cultured at 28.0±0.5°C and relative humidity of 40%-60% for 7 days, and then Add 4ml of sterile water to the slant culture medium, scrape off the spores, mix well, and make a spore suspension, the concentration of spores in the spore suspension is 10 13 pieces / ml.

[0027] Preparation of slant medium: Weigh 15g soluble starch, 2g sucrose, 3g beef extract, 1g potassium dihydrogen phosphate, 1g sodium chloride, 20g agar, add water to 1000ml, and then use 40% sodium hydroxide Adjust the pH of the solution to 7.0±0.2, and sterilize at 121°C for 20 minutes. After the sterilization is completed, divide them into several test tubes, spread them on a slant, and obtain the slant culture medium after solidification.

[0028] Pretrea...

Embodiment 2

[0033] Example 2 Bacterial classification retest

[0034] Preparation of slant medium: 10g soluble starch, 3g sucrose, 2g beef extract, 0.3g potassium dihydrogen phosphate, 0.5g sodium chloride, 20g agar, add water to 1000ml; Adjust the pH to 7.0±0.2, and sterilize at 121°C for 20 minutes.

[0035] Preparation of seed medium: 10g glucose, 12g yeast powder, 30g fish meal, 2.5g peptone, 1.5g soybean oil, add water to 1000ml; use 40% sodium hydroxide solution to adjust the pH of the medium to 6.8±0.1, at 121 Sterilize at ℃ for 20min.

[0036] Preparation of fermentation medium: 20g corn starch, 40g corn steep liquor, 5g yeast powder, 10g glucose, 20g fish meal, 1g ammonium sulfate, 3g potassium dihydrogen phosphate, 5g potassium chloride, 1g sodium hydroxide, 3g calcium carbonate, 1g poly Ether antifoaming agent, 0.2g barbiturate, add water to make up to 1000ml; use 40% sodium hydroxide solution to adjust the pH of the medium to 7.0±0.1, and sterilize at 121°C for 20min.

[00...

Embodiment 3

[0038] Example 3 Bacterial classification retest

[0039] Preparation of slant medium: 15g soluble starch, 2g sucrose, 3g beef extract, 1.2g potassium dihydrogen phosphate, 1g sodium chloride, 18g agar, add water to 1000ml; use 40% sodium hydroxide solution to adjust the pH of the medium Adjust to 7.0±0.2, and sterilize at 121°C for 20min.

[0040] Preparation of seed medium: 15g glucose, 10g yeast powder, 20g fish meal, 3g peptone, 0.5g soybean oil, add water to 1000ml; use 40% sodium hydroxide solution to adjust the pH of the medium to 6.8±0.1, at 121℃ Sterilize for 20 minutes.

[0041] Preparation of fermentation medium: 30g cornstarch, 25g corn steep liquor, 16g yeast powder, 5g glucose, 10g fish meal, 5g ammonium sulfate, 1g potassium dihydrogen phosphate, 1g potassium chloride, 3g sodium hydroxide, 5g calcium carbonate, 3g poly Ether antifoaming agent, 0.6g barbiturate, add water to make up to 1000ml; use 40% sodium hydroxide solution to adjust the pH of the medium to ...

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Abstract

The invention discloses a spectinomycin high-producing strain, a screening method of the strain and an application of the strain in the production of spectinomycin. The spectinomycin high-producing strain disclosed by the invention is named streptomycesspectablies DC15-01, is preserved in China general microbiological culture collection center (CGMCC) with the collection number CGMCC NO.6221, and the collection date is 14th, June, 2012. The spectinomycin high-producing strain screening method provided by the invention can fast screen the high-producing strain and is easy to implement; furthermore, the fermentation yield of the spectinomycin of the DC15-01 strain provided by the invention is improved by 30.6%.

Description

technical field [0001] The invention relates to a bacterial strain for producing antibiotics, a screening method thereof and its application in the field of fermentation, in particular to a spectinomycin high-yielding bacterial strain, its preparation method and application. Background technique [0002] Spectinomycin (Spectniomycni) is a species of Streptomyces spectacle ( Streptomyces spectabilis ) The secondary metabolites produced after aerobic fermentation are aminoglycoside broad-spectrum antibiotics, which have strong inhibitory ability to Gram-positive bacteria and Gram-negative bacteria, especially the strongest effect on Neisseria gonorrhoeae , It is also highly sensitive to penicillin and tetracycline-resistant gonorrhoeae. Spectinomycin is generally non-allergic, with few side effects and good safety. Spectinomycin can also be used for animal treatment, and can promote animal growth, it is a good animal feed additive. [0003] At present, the method of breedin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N13/00C12P17/18C12R1/465
Inventor 王绘砖杨丽锋魏伟
Owner 河北圣雪大成唐山制药有限责任公司
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