Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Selenium ion fluorescent probe, and preparation method and application thereof

A fluorescent probe and ion technology, applied in the field of environmental analysis, can solve problems such as analyte loss, long pH color development time, pollution, etc., and achieve the effect of low cost, easy availability of raw materials, and good selectivity

Inactive Publication Date: 2013-10-02
JILIN UNIV
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction process may cause analyte loss and contamination, and Se-DAN complexation requires pH optimization and long color development time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Selenium ion fluorescent probe, and preparation method and application thereof
  • Selenium ion fluorescent probe, and preparation method and application thereof
  • Selenium ion fluorescent probe, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of selenium ion fluorescent probe.

[0031]Weigh 1.916g (4.0mmol) of Rhodamine 6G and dissolve it in 20mL of hot ethanol solution, add 3mL (28mmol) of diethylenetriamine, and heat to reflux until the green fluorescence of the solution disappears. After evaporating part of the ethanol, add pure water while sonicating, and a light pink solid is formed. When the amount of solid precipitation no longer increases, stop adding pure water. Then perform suction filtration and wash with water 2 to 3 times. Dissolve the light pink solid obtained by suction filtration in acetonitrile, remove water with anhydrous sodium sulfate, heat and filter, and perform rotary evaporation on the filtrate to obtain a light pink solid, which is the bottom ring modified derivative of Rhodamine 6G, with a yield of about 76 %.

[0032] The characterization of the bottom ring-modified derivative of the product rhodamine 6G, the mass spectrum is shown in figure 1 , see NMR ...

Embodiment 2

[0033] Embodiment 2 Measurement of fluorescence properties of selenium ion fluorescent probe

[0034] The Se fluorescent probe prepared in Example 1 was dissolved in absolute ethanol to prepare a 10 mmol / L stock solution. Take 30 L from the stock solution and add it to a 5 mL centrifuge tube, add 30 L selenium standard solution (100 μmol / L) and dilute it to 3 mL with HEPES buffer solution (0.1 mol / L, pH=7.5), and measure its fluorescence properties. Fluorescence spectra such as image 3 shown. Depend on image 3 It can be seen that the fluorescence maximum excitation wavelength of the selenium fluorescent probe is 530nm, and the maximum emission wavelength is 550nm.

Embodiment 3

[0035] Example 3 Before and after the selenium ion fluorescent probe is mixed with selenium, the fluorescence intensity at the maximum emission wavelength of the fluorescent probe changes with time.

[0036] a. Take 30 L from the fluorescent probe stock solution in Example 2 and add it to a 5 mL centrifuge tube, add HEPES buffer solution (0.1mol / L, pH=7.5) to dilute to 3 mL, and detect the fluorescent probe at the maximum fluorescence emission wavelength. The change of needle fluorescence intensity with time.

[0037] b. Take 30 L from the fluorescent probe stock solution in Example 2 and add it to a 5mL centrifuge tube, add 30 L selenium standard solution (100 μmol / L) and dilute it with HEPES buffer solution (0.1mol / L, pH=7.5) To 3mL, detect the change of the fluorescence intensity of the fluorescent probe at the maximum fluorescence emission wavelength with time.

[0038] Such as Figure 4 As shown, the fluorescence intensity increases rapidly within 30 minutes of mixing t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a selenium ion fluorescent probe, and a preparation method and application thereof, belonging to the technical fields of environmental analysis and biological analysis detection. The selenium ion fluorescent probe is a bottom-ring-modified derivative of rhodamine 6G, and the molecular formula is C30H37N5O2. The preparation method adopts a method of aminolyzing ester group of rhodamine 6G for recyclization to synthesize the specificity-responsive selenium ion fluorescent probe molecules. The selenium ion fluorescent probe is applied to sensing detection of selenium content in an aquatic environment system or medicinal or biological cell system. The synthesis of the selenium ion fluorescent probe only needs one reaction step, and thus, the invention has the advantages of simple after-treatment, accessible raw material and low cost; and the selenium ion fluorescent probe has the characteristics of favorable selectivity, strong metallic ion interference resistance and the like, is a simple, quick and sensitive selenium ion specific detection reagent, has the function of chromophoric sensing, and thus, has wide application prospects in the field of biological and ionic detection.

Description

technical field [0001] The invention relates to the technical fields of environmental analysis and biological analysis and detection, in particular to a highly selective fluorescent probe capable of quantitatively detecting the selenium content in a sample and a synthesis method thereof. Background technique [0002] Selenium is a non-metal element of great significance in industry, environment, biology and toxicology. It is also an essential nutrient required by mammalian organisms. Its compounds play an important catalytic role in metabolic reactions, such as participating in the synthesis of proteins. More than 50 kinds of proteins in bacteria, archaea, and mammals contain selenium. In addition, selenium also has other functional effects, such as anti-cancer and resistance to the toxic and side effects of other heavy metal ions. However, selenium has dual effects. Only selenium in a certain concentration range is beneficial to organisms including humans. Excessively hig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09K11/06C07D491/107G01N21/64
Inventor 冯国栋代艳娜
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com