Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof

A microorganism and soil technology, applied in the field of environmental pollution control, can solve the problems of limited application of phytoremediation technology, limited absorption capacity, low biomass, etc., and achieve the effect of promoting growth and root extraction of heavy metals, low cost, and good restoration effect.

Active Publication Date: 2013-10-16
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, phytoremediation also has certain defects, such as: heavy metal-enriched plants usually have low biomass and slow growth; they are selective to metals and are not suitable for the treatment of multiple heavy metal pollution; the absorption capacity of plants to heavy metals is often limited by soil Limited by environmental and climatic conditions; in addition, pollutants may return to the soil through fallen leaves
These all limit the application of phytoremediation technology in the treatment of heavy metal contaminated soil.

Method used

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  • Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof
  • Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof
  • Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening, identification and biological characteristic analysis of cadmium-resistant bacteria

[0023] A plant leaf was collected from the Dabaoshan tailings area (contaminated area) in Shenzhen, rinsed with deionized water, soaked in 70% alcohol for 20 seconds, then soaked in 2.5% sodium hypochlorite for 10 minutes, and rinsed repeatedly with sterile water. The rinsed sterile water was spread on the plate of solid LB medium (10g protein powder, 5g yeast extract, 10g NaCl, 1000mL distilled water). If there is no microbial growth after cultivation, it indicates that the disinfection is complete. On the aseptic operating table, take an appropriate amount of the above-mentioned thoroughly sterilized plant tissue into a sterile mortar, add 1mL of 0.9% sodium chloride solution, grind it thoroughly, and then dilute it in a 10-fold gradient series for 10-10 6 Times, spread on solid LB medium, culture for 48h, pick different forms of bacterial isolates for purificat...

Embodiment 2

[0026] The enrichment ability of cadmium in the soil of embodiment 2 green beetle seeds

[0027] The soil used in the pot experiment was collected from a local mushroom plantation in Nanshan District, Shenzhen. After removing foreign matter, it was air-dried indoors, ground, mixed, and passed through a 4mm sieve. The seeds of Pleurotus chinensis are first germinated on wet filter paper, then transferred to the seedling medium and cultivated to the 4-6 leaf stage, and set aside.

[0028]The test uses plastic pots with a diameter of about 15cm, and each pot is filled with about 2kg of soil. The heavy metal cadmium is added into the pot in the form of a solution and mixed evenly. The contaminated soil with cadmium content of 10mg / kg, 30mg / kg and 60mg / kg is prepared, and the same amount of soil is planted respectively. At the 4-6 leaf stage of the plant, weeds were planted. At the same time, the soil without cadmium was used as the control, and the soil water content was kept at 7...

Embodiment 3

[0044] Example 3 The joint enrichment of cadmium in soil by Semen chinensis-cadmium-tolerant bacteria

[0045] First, Klebsiella sp. (Klebsiella sp.) DE2 used for inoculation in Example 1 was activated, the strain was activated using solid LB medium, cultured at 30°C for 24h, picked colonies and inserted into bean sprouts juice medium, 30°C, 150r / min shaking culture for 48h to prepare the fermentation liquid, and dilute the bacterial liquid to a concentration of 1×10 with the blank bean sprouts juice medium 8 CFU / mL, spare.

[0046] Plastic pots with a diameter of about 15 cm were used in the test, and each pot was filled with about 2 kg of soil. The heavy metal cadmium was added into the pot in the form of a solution and mixed evenly to prepare 100 mg / kg of cadmium-contaminated soil. Adjust the water content of the soil in the pot to about 70% to 85% of the maximum water holding capacity. After a week of natural balance, transplant the seedlings of the 4-6 leaf stage with b...

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Abstract

The invention relates to a method for associating plants and microorganisms to accumulate heavy metal cadmium in soil. The method comprises the following steps: firstly planting cadmium accumulated plants in cadmium polluted soil; then inoculating cadmium-resistant bacteria in rhizosphere soil of the cadmium accumulated plants; conventionally culturing, watering and moisturizing; enriching cadmium in cadmium accumulated soil by utilizing the absorption effect of the cadmium accumulated plants to the cadmium and reinforcing effect of the cadmium-resistant bacteria; repeating the process so that the purpose of repairing the cadmium polluted soil can be achieved. The soil repairing method is good in effect, low in cost, easy to manage and operate, and free of secondary pollution, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of environmental pollution control, in particular to utilizing the cadmium-enriching plant Pleurotus chinensis to enrich heavy metal cadmium in soil, and inoculating cadmium-resistant bacteria in the rhizosphere to strengthen the cadmium enrichment ability of Pleurotus chinensis, which is suitable for heavy metal cadmium pollution in soil Quick and safe fixes. Background technique [0002] With the rapid development of industry and economy, the soil in many areas of our country is polluted by heavy metals to varying degrees, and the polluted area and degree of pollution are constantly expanding. According to the survey of the Ministry of Agriculture, the land area polluted by heavy metals in my country accounts for 100% of the sewage irrigation area. 64.8% of the total area. Cadmium that pollutes the environment mainly comes from waste water, waste gas and waste residues discharged by metal mines, non-ferrou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 刘淑杰陈福明刘莉华吴秉奇杨小龙
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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