Preparation method of fly maggot peptides
A technology of fly maggot peptide and maggot peptide, applied in the field of microorganisms, can solve the problems of high cost, low conversion rate, complicated process and the like, and achieve the effects of low manufacturing cost, high conversion rate and simple method.
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Embodiment 1
[0030] PDA medium was sterilized at 0.1 MPa for 20 min, and paved on a slope for later use.
[0031] Preparation of medium with fly maggot protein as the only nitrogen source: in terms of mass percentage, fly maggot protein 2-3%, glucose or other sugar 1-3%, disodium hydrogen phosphate 1.0-2.0%, dihydrogen phosphate Sodium 0.5-1.5%, the rest is water.
[0032] Preparation method: take a certain quality of disodium hydrogen phosphate and sodium dihydrogen phosphate, dissolve in water, add fresh fly maggots or dry fly maggots to soak thoroughly, add, homogenate, remove solids, measure protein concentration, make it in 2- 3%, add 1-3% glucose or other sugars, stir evenly, and sterilize at 0.1 Mpa for 20 min.
[0033] Preparation of myia peptide:
[0034] a. Inoculate Aspergillus oryzae (Aspergillus oryzae) 3042 into the above-mentioned PDA medium, and culture at 32°C for 48-72 hours with aeration to obtain Aspergillus oryzae spores;
[0035] Aspergillus oryzae 3042 was purchas...
Embodiment 2
[0040] a. Fill the test tube with an appropriate amount of Caspian culture medium, sterilize at 0.1 MPa for 20 minutes, and make a slant for use; put the preserved Aspergillus oryzae (3.042) into the slant, and cultivate it in a constant temperature incubator at 32 ℃ until it is full of spores.
[0041] b. Put an appropriate amount of grown Aspergillus oryzae 3.042 into a Erlenmeyer flask with a culture medium with fly maggot protein as the only nitrogen source, and cultivate for 96 hours at 32°C with shaking at 180 r / min. Centrifuge at 6000r / min for 20min to remove mycelium, and the supernatant is a fly maggot peptide solution;
[0042] The preparation method of the above-mentioned culture medium with fly maggot protein as the only nitrogen source: after fully soaking fresh fly maggot or dry fly maggot, add pH6.5 buffer solution, homogenate, without centrifugation, adjust its protein concentration to 2.5%, add 2 % glucose, fill each 500mL Erlenmeyer flask with 50mL, and steri...
Embodiment 3
[0046] a. Aspergillus niger (Aspergillus niger) 3350 was inoculated into the slant culture medium of Chapei, and cultured with aeration at 30°C for 4 days to obtain Aspergillus niger spores;
[0047]b. Inoculate Aspergillus niger spores into the culture medium in which the fly maggot protein is the only nitrogen source, shake the flask at 200r / min at 30-32°C, incubate with ventilation for 80-100h, and centrifuge at 6000r / min for 20min to remove insoluble solids Mycelia, collect the supernatant (i.e. myia peptide solution);
[0048] The above-mentioned preparation method of the substratum taking the fly maggot protein as the sole nitrogen source is:
[0049] Fully soak the fresh or dried fly maggots, add tap water, homogenate, without centrifugation, so that the concentration of soluble protein is in the range of 2.0-4.5%, directly add 1-3% sucrose or molasses, hydrochloric acid to adjust the pH to about 5.5, every 50mL of liquid in a 500mL Erlenmeyer bottle, sterilized at 0.1...
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