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Method for preparing tissue engineering cell culture support

A cell culture and tissue engineering technology, applied in the field of tissue engineering cell culture scaffolds, can solve the problems of difficult to increase porosity, insufficient manufacturing process, high cost, etc., to achieve improved biocompatibility, excellent mechanical properties, good biological compatibility effect

Active Publication Date: 2013-12-11
深圳市旷逸生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has a complicated process and high cost, and the pore size distribution of the prepared porous titanium scaffold is not uniform, and the porosity is difficult to be greatly improved.
[0006] In summary, it is difficult to achieve the unification of excellent mechanical properties, uniform pore size distribution and good connectivity pore structure by using the traditional methods of preparing porous metals to prepare tissue engineering cell culture scaffolds. Disadvantages such as obvious insufficient manufacturing process and high production cost

Method used

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  • Method for preparing tissue engineering cell culture support

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Take 1.0 g of 316L stainless steel fiber with a diameter of 30 μm, cut it into short fibers with a length of 5 mm, fill it randomly into a barrel-shaped mold with an inner diameter of 20 mm, and press it from above with a rod-shaped indenter, according to the design porosity 90% and the thickness of the preform is 4 mm to obtain a cylindrical cell culture scaffold preform with a thickness of 4 mm. Put the cylindrical cell culture scaffold preform obtained after pressing into oxalic acid aqueous solution (5% W / V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, each ultrasonic cleaning for 10 minutes. Then, the preform was put into a vacuum drying oven at a temperature of 60° C. and kept at a constant temperature for 2 hours. Next, clamp the prefabricated body into the disc-type pressurizing device, adjust the nut to tighten the pressing piece to keep the thickness of the preformed body at 4mm, and place the disc-type pressurizing device with the p...

Embodiment 2

[0053] Take 1.0 g of 316L stainless steel fiber with a diameter of 30 μm, cut it into short fibers with a length of 5 mm, and then evenly disperse and fill it in a barrel-shaped mold with an inner diameter of 20 mm, and press it with a rod-shaped indenter. According to the design porosity 80% and a preform thickness of 2mm are pressed to obtain a cylindrical cell culture scaffold preform with a thickness of 2mm. . Put the cylindrical cell culture scaffold preform obtained after pressing into oxalic acid aqueous solution (5% W / V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, each ultrasonic cleaning for 10 minutes. Then, the preform was put into a vacuum drying oven at a temperature of 60° C. and kept at a constant temperature for 2 hours. Next, clamp the prefabricated body into the disc-type pressurizing device, adjust the nut to tighten the pressing piece to keep the thickness of the preformed body at 2mm, and place the disc-type pressurizing device...

Embodiment 3

[0055] Take 1.0g of 316L stainless steel fiber with a diameter of 30μm, integrate it into a bundle, bend it into a "Z"-shaped fiber bundle with a total length of 15mm, and cut it (the "Z"-shaped fiber bundle is bent twice The final three fiber bundles are formed, each length is equal to 5mm), and then the "Z"-shaped fiber bundles with equal lengths are dispersed into a single "Z"-shaped fiber and then randomly placed in a conical container to form a structure , and then fill this structure in a barrel-shaped mold with an inner diameter of 20 mm. According to the design porosity of 90% and the prefabricated body thickness of 4 mm, the prefabricated body of the cylindrical cell culture scaffold with a thickness of 4 mm is obtained. Put the cylindrical cell culture scaffold preform obtained after pressing into oxalic acid aqueous solution (5% W / V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, each ultrasonic cleaning for 20 minutes. Then, the preform wa...

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Abstract

The invention provides a method for preparing a tissue engineering cell culture support. The method comprises the steps of using metal fibers as raw materials, preprocessing the metal fibers, and conducting compression forming on the preprocessed fibers or structural bodies to obtain a prefabricated body of the cell culture support; then, conducting cleaning and vacuum drying processing on the prefabricated body; then, placing the prefabricated body into a disc-type pressurization device to be sintered under the protection of vacuum or argon, and obtaining the tissue engineering cell culture support. The tissue engineering cell culture support prepared by the method has better biocompatibility, excellent mechanical properties and the higher porosity, and is provided with a communicated hole structure, and three-dimensional culture of cells and implantation in vivo of tissue engineering are facilitated.

Description

technical field [0001] The invention relates to a tissue engineering cell culture support. Background technique [0002] Tissue engineering cell culture scaffold is a three-dimensional porous open network framework, which can guide cells to carry out activities such as adhesion, growth, proliferation and differentiation, so that they can finally form the desired three-dimensional tissue or organ. Tissue engineering cell culture scaffold plays the role of simulating extracellular matrix. It not only provides three-dimensional space and metabolic place for cell growth, but also affects the biological behavior and culture efficiency of cells, and at the same time determines whether it can be well with the body after implantation. Adaptation and combination, and then affect the effect of the whole tissue repair. Therefore, the preparation of tissue engineering cell culture scaffold is one of the most critical links in tissue engineering research. [0003] At present, tissue en...

Claims

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Application Information

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IPC IPC(8): B22F5/10B22F3/16A61L27/04A61L27/06A61L27/56
CPCC12M25/14
Inventor 尹大川李大为何进王鹏燕何凤利陈瑞卿
Owner 深圳市旷逸生物科技有限公司
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