Method for preparing tissue engineering cell culture support

A cell culture and tissue engineering technology, applied in the field of tissue engineering cell culture scaffolds, can solve the problems of difficult to increase porosity, insufficient manufacturing process, high cost, etc., to achieve improved biocompatibility, excellent mechanical properties, good biological compatibility effect

Active Publication Date: 2013-12-11
深圳市旷逸生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has a complicated process and high cost, and the pore size distribution of the prepared porous titanium scaffold is not uniform, and the porosity is difficult to be greatly improved.
[0006] In summary, it is difficult to achieve the unification of excellent mechanical pr

Method used

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  • Method for preparing tissue engineering cell culture support

Examples

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Example Embodiment

[0050] Example 1

[0051] Take 1.0g of 316L stainless steel fiber with a diameter of 30μm, cut it into short fibers with a length of 5mm, and randomly fill it into a barrel-shaped mold with an inner diameter of 20mm, and use a rod-shaped indenter to press from above, according to the designed porosity It is compressed to 90% and the thickness of the preform is 4mm to obtain a cylindrical cell culture scaffold preform with a thickness of 4mm. The cylindrical cell culture scaffold preform obtained after pressing was sequentially put into oxalic acid aqueous solution (5% W / V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, each time ultrasonic cleaning was 10 minutes. Then, put the preform into a vacuum drying oven at a temperature of 60° C. and maintain a constant temperature for 2 hours. Next, clamp the preform into the wafer press, adjust the nut to tighten the tablet to keep the preform at a thickness of 4mm, and place the wafer press with the preform in...

Example Embodiment

[0052] Example 2

[0053] Take 1.0g of 316L stainless steel fiber with a diameter of 30μm, cut it into short fibers with a length of 5mm, and evenly disperse and fill it in a barrel-shaped mold with an inner diameter of 20mm. Press with a rod-shaped indenter. The porosity is 80% and a preform with a thickness of 2 mm were pressed to obtain a cylindrical cell culture scaffold preform with a thickness of 2 mm. . The cylindrical cell culture scaffold preform obtained after pressing was sequentially put into oxalic acid aqueous solution (5% W / V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, each time ultrasonic cleaning was 10 minutes. Then, put the preform into a vacuum drying oven at a temperature of 60° C. and maintain a constant temperature for 2 hours. Next, clamp the preform into the wafer press device, adjust the nut to tighten the tablet so that the preform maintains a thickness of 2 mm, and place the wafer press device with the preform in the vacu...

Example Embodiment

[0054] Example 3

[0055] Take 1.0g of 316L stainless steel fiber with a diameter of 30μm, integrate it into a bundle, bend it into a "Z"-shaped fiber bundle with a total length of 15mm, and then cut it (the "Z"-shaped fiber bundle is bent twice The three fiber bundles formed afterwards, each of which is equal in length is 5mm), and then the "Z"-shaped fiber bundles of equal length are dispersed into single "Z"-shaped fibers and randomly placed in a conical container to form a structure , And then fill this structure into a barrel-shaped mold with an inner diameter of 20 mm. According to the design porosity of 90% and the preform thickness of 4mm, the compaction is performed to obtain a cylindrical cell culture scaffold preform with a thickness of 4mm. The cylindrical cell culture scaffold preform obtained after pressing was sequentially put into oxalic acid aqueous solution (5% W / V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, and ultrasonic cleaning ...

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Abstract

The invention provides a method for preparing a tissue engineering cell culture support. The method comprises the steps of using metal fibers as raw materials, preprocessing the metal fibers, and conducting compression forming on the preprocessed fibers or structural bodies to obtain a prefabricated body of the cell culture support; then, conducting cleaning and vacuum drying processing on the prefabricated body; then, placing the prefabricated body into a disc-type pressurization device to be sintered under the protection of vacuum or argon, and obtaining the tissue engineering cell culture support. The tissue engineering cell culture support prepared by the method has better biocompatibility, excellent mechanical properties and the higher porosity, and is provided with a communicated hole structure, and three-dimensional culture of cells and implantation in vivo of tissue engineering are facilitated.

Description

technical field [0001] The invention relates to a tissue engineering cell culture support. Background technique [0002] Tissue engineering cell culture scaffold is a three-dimensional porous open network framework, which can guide cells to carry out activities such as adhesion, growth, proliferation and differentiation, so that they can finally form the desired three-dimensional tissue or organ. Tissue engineering cell culture scaffold plays the role of simulating extracellular matrix. It not only provides three-dimensional space and metabolic place for cell growth, but also affects the biological behavior and culture efficiency of cells, and at the same time determines whether it can be well with the body after implantation. Adaptation and combination, and then affect the effect of the whole tissue repair. Therefore, the preparation of tissue engineering cell culture scaffold is one of the most critical links in tissue engineering research. [0003] At present, tissue en...

Claims

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Application Information

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IPC IPC(8): B22F5/10B22F3/16A61L27/04A61L27/06A61L27/56
CPCC12M25/14
Inventor 尹大川李大为何进王鹏燕何凤利陈瑞卿
Owner 深圳市旷逸生物科技有限公司
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