Method for preparing tissue engineering cell culture support

[0050] Example 1

[0051] Take 1.0g of 316L stainless steel fiber with a diameter of 30μm, cut it into short fibers with a length of 5mm, and randomly fill it into a barrel-shaped mold with an inner diameter of 20mm, and use a rod-shaped indenter to press from above, according to the designed porosity It is compressed to 90% and the thickness of the preform is 4mm to obtain a cylindrical cell culture scaffold preform with a thickness of 4mm. The cylindrical cell culture scaffold preform obtained after pressing was sequentially put into oxalic acid aqueous solution (5% W/V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, each time ultrasonic cleaning was 10 minutes. Then, put the preform into a vacuum drying oven at a temperature of 60° C. and maintain a constant temperature for 2 hours. Next, clamp the preform into the wafer press, adjust the nut to tighten the tablet to keep the preform at a thickness of 4mm, and place the wafer press with the preform in the vacuum (vacuum degree is 1.0× 10 -3 Sintering under Pa) conditions, the sintering temperature is 1200℃, and the sintering time is 2h. After sintering, the furnace is cooled to room temperature, and finally a stainless steel fiber-based tissue engineering cell culture scaffold is obtained. The cell culture scaffold has a pore size of 40-200μm observed by scanning electron microscope, and the porosity measured by medium immersion method is 90.2%. Pore ​​connectivity is good and mechanical properties are excellent.

[0052] Example 2

[0053] Take 1.0g of 316L stainless steel fiber with a diameter of 30μm, cut it into short fibers with a length of 5mm, and evenly disperse and fill it in a barrel-shaped mold with an inner diameter of 20mm. Press with a rod-shaped indenter. The porosity is 80% and a preform with a thickness of 2 mm were pressed to obtain a cylindrical cell culture scaffold preform with a thickness of 2 mm. . The cylindrical cell culture scaffold preform obtained after pressing was sequentially put into oxalic acid aqueous solution (5% W/V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, each time ultrasonic cleaning was 10 minutes. Then, put the preform into a vacuum drying oven at a temperature of 60° C. and maintain a constant temperature for 2 hours. Next, clamp the preform into the wafer press device, adjust the nut to tighten the tablet so that the preform maintains a thickness of 2 mm, and place the wafer press device with the preform in the vacuum (vacuum degree is 1.0× 10 -3 Sintering under Pa) conditions, the sintering temperature is 1200℃, and the sintering time is 2h. After sintering, the furnace is cooled to room temperature, and finally a stainless steel fiber-based tissue engineering cell culture scaffold is obtained. The cell culture scaffold has a pore size of 40-150μm observed by scanning electron microscope, and the porosity measured by medium immersion method is 79.7%. Pore ​​connectivity is good and mechanical properties are excellent.

[0054] Example 3

[0055] Take 1.0g of 316L stainless steel fiber with a diameter of 30μm, integrate it into a bundle, bend it into a "Z"-shaped fiber bundle with a total length of 15mm, and then cut it (the "Z"-shaped fiber bundle is bent twice The three fiber bundles formed afterwards, each of which is equal in length is 5mm), and then the "Z"-shaped fiber bundles of equal length are dispersed into single "Z"-shaped fibers and randomly placed in a conical container to form a structure , And then fill this structure into a barrel-shaped mold with an inner diameter of 20 mm. According to the design porosity of 90% and the preform thickness of 4mm, the compaction is performed to obtain a cylindrical cell culture scaffold preform with a thickness of 4mm. The cylindrical cell culture scaffold preform obtained after pressing was sequentially put into oxalic acid aqueous solution (5% W/V), acetone, absolute ethanol and deionized water for ultrasonic cleaning, and ultrasonic cleaning was performed for 20 minutes each time. Then, the preform is put into a vacuum drying oven at a temperature of 60°C and kept at a constant temperature for 2 hours. Next, clamp the preform into the wafer press device, adjust the nut to tighten the tablet so that the preform maintains a thickness of 4mm, and place the wafer press device with the preform in the vacuum (vacuum degree is 1.0× 10 -3 Sintering under Pa) conditions, the sintering temperature is 1200℃, and the sintering time is 2h. After sintering, the furnace is cooled to room temperature, and finally a stainless steel fiber-based tissue engineering cell culture scaffold is obtained. The cell culture scaffold has a pore size of 50-300μm observed by scanning electron microscope, and the porosity measured by medium immersion method is 90.1%. Pore ​​connectivity is good and mechanical properties are excellent.