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Aspergillus niger C2 and microbial inoculum thereof

A technology of Aspergillus niger and microbial agent, applied in the field of microorganisms, can solve the problems of low utilization rate of phosphate fertilizer and decrease of production increase potential, and achieve obvious effect of promoting growth and saving phosphate fertilizer resources

Active Publication Date: 2013-12-11
北京中加保罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention mainly aims at the problems of phosphorus deficiency, low utilization rate of phosphate fertilizer, and decreased yield increase potential in my country, and provides a widely adaptable high-efficiency phosphate-dissolving bacterium and the microbial agent prepared therefrom

Method used

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  • Aspergillus niger C2 and microbial inoculum thereof
  • Aspergillus niger C2 and microbial inoculum thereof
  • Aspergillus niger C2 and microbial inoculum thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and cultivation of Aspergillus niger C2

[0020] (1) Aspergillus niger C2 medium

[0021] Fermentation broth formula (kg / t fermentation broth): dipotassium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.1, sodium chloride 4.3, magnesium sulfate 0.1, starch 8.6, beef extract 4.3, peptone 4.3, yeast powder 4.3, sucrose 8.6, sulfuric acid Ammonium 2.6, calcium carbonate 10, ferrous sulfate 0.001, soybean oil 6.8, prepared with water.

[0022] (2) Cultivation process of Aspergillus niger C2

[0023] Inoculate Aspergillus niger C2 strain on beef juice peptone medium, culture at 28-30°C for 48h, then transfer to a 1000mL Erlenmeyer flask, and culture at 30°C, 220r / min for 36h; transfer to 50L with 5% inoculum In the seed tank, under the conditions of 220r / min, pH7.5, ventilation 0.5vvm, after culturing for 2 days, transfer it to a 500L fermenter at 200r / min, pH7.5, ventilation Under the condition of 0.7~1.0vvm, cultivate for 3 days; after the ...

Embodiment 2

[0024] Embodiment 2 The preparation of high-efficiency phosphorus-dissolving microbial bacterial agent

[0025] 1. Cultivation of Aspergillus niger C2

[0026] (1) Composition of medium (kg / t fermentation broth)

[0027] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg,K 2 HPO 4 0.2kg, NaCl2.5kg, MgSO 4 ·7H 2 O0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0028] (2) Fermentation and cultivation process

[0029] Aspergillus niger C2 was inoculated on potato sucrose (PDA) medium and cultured at 25-28°C for 3 days. Transfer the spores of Aspergillus niger C2 into a 1000mL Erlenmeyer flask, and conduct liquid fermentation at 26-28°C and 220r / min for 36h. Then insert 50L seed tank according to 1% inoculum amount, under the condition of 200r / min, pH7.0, ventilation rate 0.7vvm, cultivate after 36h, then transfer to 500L fermenter according to 10v / v% inoc...

experiment example 3

[0034] Experimental Example 3 Effect of Aspergillus niger C2 dissolving insoluble phosphorus

[0035] 1. Aspergillus niger C2 was used, and Bacillus megaterium (ATCC14581) and Penicillium bayley (ATCC20851) were used as controls. Under indoor conditions, tricalcium phosphate was used as insoluble phosphorus, and the plate solid medium was cultured for 5 days to measure its phosphorus dissolving ability.

[0036] The ratio of the phosphorus-dissolving circle to the colony diameter of Aspergillus niger C2 reached 3.10, and the water-soluble soluble (P 2 o 5 ) is 15.98mg; the phosphorus-dissolving circle of bacterial strain ATCC20851 and ATCC14581 is 1.06 and 1.30 with the colony diameter ratio respectively, and the phosphorus-dissolving amount in the phosphorus-dissolving circle (P 2 o 5 ) were 6.31 and 2.11 mg, respectively, both lower than the strain A. niger C2 (Table 1).

[0037] Table 1 Phosphorus-soluble strains containing Ca 3 (PO 4 ) 2 The ratio of the diameter of...

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Abstract

The invention discloses an Aspergillus niger C2 of which the collection number is CGMCC NO.7511 and the collection date is July 5th, 2013. The Aspergillus niger C2 is separated from farmland soil in Hengshui Cicy Hebei Province in China, and has been collected at China General Microbiological Culture Collection Center; and the address is Institute of Microbiology in Chinese Academy of Sciences, Beichenxi Road Yard 3 No.3, Chaoyang District, Beijing City. The invention also discloses a microbial inoculum containing the Aspergillus niger C2. The microbial inoculum disclosed by the invention has obvious growth promotion effect on plants without being inhibited by the application of the fertilizer, can be used under the condition of normally using the fertilizer, and has important meanings for phosphate fertilizer resource saving for Chinese agricultural production, continuous yield increase of grains and protection of ecological environment of farmland.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a high-efficiency phosphorus-dissolving Aspergillus niger C2 and a microbial agent thereof. Background technique [0002] More than 90% of the arable land in my country is deficient in phosphorus. The use of phosphorus fertilizer is the main measure to solve crop yield increase. Phosphorus fertilizer has increasingly become a bottleneck restricting the sustainable development of my country's agriculture. my country is the world's largest consumer of chemical fertilizers and the largest consumer of phosphate fertilizers, with an annual consumption of about 11 million tons of phosphate fertilizers. As we all know, my country is short of phosphate rock resources, with reserves of 15.198 billion tons. In another 20 years, the medium and high-grade phosphate rock will be exhausted (Li Zhining, 2003a, Li Zhining, 2003b), and we have to rely on purchasing high-priced foreign ph...

Claims

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Application Information

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IPC IPC(8): C12N1/14C09K17/00C05G3/00C12R1/685C09K101/00
Inventor 范丙全武庆
Owner 北京中加保罗生物科技有限公司
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