A kind of non-alcoholic fatty liver disease model preparation method

A fatty liver disease, non-alcoholic technology, applied in the field of human non-alcoholic fatty liver disease (NAFLD) model preparation, can solve the problems of large developmental differences, difficult to control, high sugar, etc., to achieve strong pertinence, promote occurrence, and reasonable route Effect

Active Publication Date: 2015-08-26
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage is to explore the mechanism of a certain factor in the pathogenesis of human NAFLD; the disadvantage is high sugar, high fat or gene knockout, which is quite different from the occurrence and development of human NAFLD, and it is difficult to control
At present, there is no literature showing the establishment of a non-gene knockout, non-high-fat, non-high-glucose metabolic regulation model of NAFLD, and the establishment of this model is more beneficial for exploring the mechanism of abnormal fat metabolism and promoting the prevention and treatment of NAFLD

Method used

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  • A kind of non-alcoholic fatty liver disease model preparation method
  • A kind of non-alcoholic fatty liver disease model preparation method
  • A kind of non-alcoholic fatty liver disease model preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The livers of the model mice and control mice were compared after 2 weeks, and the size and shape of the livers were as follows: Figure 5 Shown: Fatty liver animal model sees massive hepatic fat accumulation ( Figure 5 A). There was no change in the rat liver of the control group ( Figure 5 B). Visually, there was a clear difference in liver size.

Embodiment 2

[0052] After the two groups of mice were treated, 5ml of blood was collected from the hearts of the mice, and the serum was separated in time and stored at -20°C; the liver was removed from the mice, and the liver tissue was divided into 1. 5 cm × 1. 5 cm × 0. 3 cm , respectively placed in AAF mixed fixative solution (ethanol, acetic acid, neutral formaldehyde, V / V / V, refer to the method introduced by Xu Ping (Journal of Henan Medical University, 1999, 34 (4): 75) for fixation.

[0053] The liver tissues of the two groups of mice were routinely frozen into sections using a Leica-CM1900 cryostat and Feather disposable blades. The samples were collected, embedded in ordinary glue (frozen), sectioned, and sealed after staining.

[0054] Routine pathological examination (H&E staining) was performed on the mice of the two groups; the liver tissue of the mice was stained with oil red O method for fat accumulation, and the fat accumulation in the liver tissue was dynamically analyzed....

Embodiment 3

[0066] Dynamic analysis of liver tissue fat. The liver tissue of the two groups of rats after staining was dynamically analyzed, and the obtained dynamic analysis of liver tissue fat was compared, and it was obtained that during the establishment of the dynamic change model of fatty acids and total fatty acids, fat accumulation in liver tissue, long-chain fatty acids, short-chain fatty acids and Total fatty acid level (attached table); changes in peripheral blood, long-chain fatty acid, short-chain fatty acid and total fatty acid levels. For example, the changes of fatty acid concentration in mouse liver tissue are as follows:

[0067] group total fatty acids free fatty acid short chain fatty acids long chain fatty acids model mouse 392.16±53.08* 252.28±28.21* 61.56±23.28* 78.32±9.51* Control mice 323.78±51.29 151.46±37.81 106.92±41.16 65.80±7.73

[0068] *P<0.05

[0069] The above proves that the invention technology can be used to pr...

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Abstract

The present invention discloses a new type of non -alcoholic alcoholic fatty liver disease model preparation method, including the following steps: a. Randomly divide the rats in two groups, the first group: model group: second group: control mice; daily record status and statement of the status of the state and the status of each day;Analysis of urine, liver tissue and blood as lipid analysis; b. Treatment of experimental objects; c. Create model; d. Hepatitis tissue and blood collection; e.Slice and dyeing seal; e. Pathological examination of the model mice routine; f. Pathological examination of the control of the control mice; dynamic analysis of the liver tissue fat obtained in the steps of f) and g).The new type of non -alcoholic fatty liver disease model established by the present invention has reasonable routes, easy operation, and strong targeted. It is an effective tool for further studying non -alcoholic fatty liver.

Description

technical field [0001] The invention relates to a method for preparing a human non-alcoholic fatty liver disease (NAFLD) model. Specifically, it can be used for analysis of the mechanism of human NAFLD, evaluation of diagnostic methods, drug screening and prevention, and belongs to the medical field. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is divided into two categories: primary and secondary. The former is related to insulin resistance and genetic susceptibility, while the latter is caused by some special reasons. Excessive weight gain and overweight caused by overnutrition, obesity, diabetes, hyperlipidemia and other metabolic syndrome-related fatty liver, and cryptogenic fatty liver all belong to the category of primary non-alcoholic fatty liver disease; while nutritional Fatty liver caused by malnutrition, total parenteral nutrition, rapid weight loss after bariatric surgery, drug, environmental and industrial poisoning belongs to the cat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/205
Inventor 姚登福
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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