Long-acting enzymolysis method for vegetable proteins
A plant protease and plant protein technology, applied in the field of food processing, can solve problems such as easy inactivation of enzymes, difficult enzymatic hydrolysis, and microbial contamination
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Embodiment 1
[0028] Measure 100ml of distilled water and heat it to boiling in a 250ml beaker, add wetted 2g cornstarch (Chemical Reagent of Sinopharm Group, analytical grade), keep stirring until all dissolved and then cooled to 50°C to obtain a protease with a mass percentage of 2% Protectant solution; weigh 10g of soybean protein isolate (Solarbio, batch number: 20110520), 0.2g of sodium diacetate and 0.05g of potassium sorbate in another 250ml beaker, and at the same time add 100ml of protease protectant solution into the beaker to obtain the material Adjust the pH of the feed solution to 7.5 with 2M NaOH solution, stir until the soybean protein isolate is in a dissolved state; finally add 0.005 g of alkaline protease (Novozymes) and 0.005 g of flavored protease (Novozymes) to obtain a reaction solution; The solution was stirred at 150 rpm, and enzymatically hydrolyzed at 40 °C for 120 h; then heated to 100 °C and maintained for 5 min to inactivate the enzyme. It was determined that th...
Embodiment 2
[0030] Measure 100ml of distilled water, heat and boil in a 250ml beaker, add wetted 1g of corn starch (Chemical Reagent of Sinopharm Group), keep stirring until fully dissolved, and then cool to room temperature to obtain a protease protectant solution with a mass percentage of 1%; Weigh 1 g of soybean protein isolate (Solarbio, batch number: 20110520), 0.2 g of sodium diacetate and 0.05 g of potassium sorbate into another 250 ml beaker, and at the same time add 100 ml of protease protectant solution to the beaker to obtain a feed solution; use 2M The pH of the feed solution was adjusted to 7.5 with the NaOH solution of 100 mg, and stirred until the soybean protein isolate was in a dissolved state; finally, 0.05 g of alkaline protease (Novozymes) and 0.05 g of protamex (Novozymes) were added to obtain a reaction solution; the reaction solution was heated at 150 rpm / Stir for 12 hours at 50°C; then heat to 100°C for 5 minutes to inactivate the enzyme. The degree of hydrolysis ...
Embodiment 3
[0032] Measure 100ml of distilled water into a 250ml beaker, add wetted 5g of maltodextrin (Chemical Reagent of Sinopharm Group), keep stirring until fully dissolved and then cooled to room temperature to obtain a protease protectant solution with a mass percentage of 5%; Take 10g of peanut protein isolate (Henan Liangjian Technology Co., Ltd.), 0.2g of sodium diacetate and 0.01g of nisin in another 250ml flask, and at the same time add 100ml of protease protectant solution into the beaker to obtain a feed solution; use 2M NaOH solution and 10M hydrochloric acid solution were used to adjust the pH of the feed solution to 6.0, stirring at 150 rpm; finally, 0.5 g of papain (Guangxi Nanning Pangbo Biological Engineering Co., Ltd.) and 0.5 g of Protamex (novozymes) were added to obtain a reaction solution; The reaction solution was stirred and mixed, and enzymatically hydrolyzed at 50 °C for 48 h; then heated to 100 °C for 5 min to inactivate the enzyme. The degree of hydrolysis o...
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