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Application of recombined ganoderma lucidum immunomodulatory protein in preparing drug for treating parkinsonism

A technology for immunoregulatory proteins and Parkinson's disease, applied in drug combinations, peptide/protein components, pharmaceutical formulations, etc., can solve the problem of no PD and achieve high safety and high safety effects

Inactive Publication Date: 2014-01-29
张喜田 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no drug or treatment that can fundamentally control the progression of PD, and all existing therapies have their limitations

Method used

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  • Application of recombined ganoderma lucidum immunomodulatory protein in preparing drug for treating parkinsonism
  • Application of recombined ganoderma lucidum immunomodulatory protein in preparing drug for treating parkinsonism
  • Application of recombined ganoderma lucidum immunomodulatory protein in preparing drug for treating parkinsonism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 rLZ-8 can improve the behavioral disorder of mice caused by MPTP

[0035] 1 Experimental materials

[0036] rLZ-8 (provided by the Institute of Genetic Engineering Drugs of Jilin University), MPTP (Sigma), Madopar (Roche).

[0037] 2 Instruments and equipment

[0038] ZB-200 fatigue rotarod instrument (Chengdu Taimeng Technology Co., Ltd.), mouse climbing rod frame, mouse suspension line

[0039] 3 Experimental animals

[0040] Healthy flexible C57BL / 6 mice, male, 6-8 weeks old, weighing 20±2g, SPF grade. Purchased from Beijing Huafukang Biotechnology Co., Ltd., animal license number: SCXK Beijing 2009-0004. Raised under SPF grade conditions of Jilin University. During the experiment, the room temperature was controlled at (20±2)°C, the lighting was alternated for 12 hours, and the mice had free access to water and food.

[0041] The animals were trained in pole climbing test, rotarod test and suspension test before administration, and 60 qualified mic...

Embodiment 2

[0060] Example 2 rLZ-8 can improve the damage of dopaminergic neurons in mice caused by MPTP,

[0061] 1 Experimental materials

[0062] rLZ-8 (provided by Jilin University Zanzhi Genetic Engineering Drug Research Center), MPTP (lot number: 20111016), Madopar (Roche), rabbit anti-mouse tyrosine hydroxylase (TH) antibody, rabbit anti-mouse glue Fibrillary acidic protein (GFAP) antibody, rabbit anti-mouse ionized calcium adapter protein (Iba-1) antibody, SP immunohistochemical detection kit (the above antibodies are all from Beijing Boaosen Biotechnology Co., Ltd.), DAB chromogenic reagent Box (Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

[0063] 2 Instruments and equipment

[0064] BMJ-1 biological tissue embedding machine (Tianjin Aviation Electromechanical Company), RM2245 tissue slicer (LEICA), biological microscope (Chongqing Optoelectronics Instrument Co., Ltd.)

[0065] 3 Experimental animals

[0066] Same as Example 1

[0067] 4 Experimental methods

[00...

Embodiment 3

[0075] Example 3 rLZ-8 can inhibit MPTP-induced enhancement of oxidative stress in mouse brain and its effect on major organ indexes

[0076] 1 Experimental materials

[0077] rLZ-8 (provided by the Genetic Engineering Drug Research Center of Jilin University), MPTP (lot number: 20111016), Madopar (Roche), total protein quantitative test kit, superoxide dismutase (SOD) test kit, malondialdehyde (MDA) test kit, glutathione peroxidase (GSH-PX) test kit, nitric oxide test kit (nitrate reductase method), acetylcholinesterase test kit, ultra-trace ATPase test kit (the above kit were purchased from Nanjing Jiancheng Institute of Bioengineering).

[0078] 2 Instruments and equipment

[0079] Low temperature centrifuge (5810R), digital display constant temperature circulating water tank (Guohua HH-42 type), 752 type ultraviolet grating spectrophotometer (Shandong Gaomi Analytical Instrument Factory).

[0080] 3 Experimental animals

[0081] Same as Example 1

[0082] 4 Experime...

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Abstract

The invention discloses an application of a recombined ganoderma lucidum immunomodulatory protein in preparing a drug for treating parkinsonism. In-vivo animal tests show that the recombined ganoderma lucidum immunomodulatory protein can improve the behavioral disorder of a Parkinson animal model, has a certain neuroprotective effect on brain tissue dopaminergic neuron cell of the Parkinson animal model, and can prevent and treat parkinsonism.

Description

technical field [0001] The recombinant Ganoderma lucidum immunoregulatory protein involved in the present invention is the application of the recombinant Ganoderma lucidum immunomodulatory protein expressed by deep fermentation in the preparation of Parkinson's disease medicine with Pichia pastoris as an expression carrier through gene recombination technology, especially related to recombinant Ganoderma lucidum The application of the immunoregulatory protein in the treatment of Parkinson's disease belongs to the field of biopharmaceutical engineering. Background technique [0002] Parkinson's disease (PD) is the second most common neurodegenerative disease in middle-aged and elderly people, and it is one of the two chronic killers of the elderly. With the advent of an aging society, the prevalence of PD is increasing year by year worldwide. According to the World Parkinson's Disease Association, there are more than 4 million Parkinson's patients worldwide. At present, ther...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P25/16
CPCA61K38/16A61P25/16
Inventor 孙非梁重阳张喜田
Owner 张喜田
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