Method for preparing low-molecular-weight sturgeon chondroitin sulfate by utilizing sturgeon chine

A chondroitin sulfate, low molecular weight technology is applied in the field of preparation of chondroitin sulfate, and achieves the effects of low production cost, simple and easy physical method, and high product purity

Active Publication Date: 2014-02-05
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods of preparing low molecular weight chondroitin sulfate by irrad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] (6) Preparation process of low molecular weight sturgeon chondroitin sulfate: prepare the chondroitin sulfate obtained in step (5) into a 5%-15% solution, add oxalic acid to control the oxalic acid concentration in the system at 5%-15%, and the degradation time is 1 -2 hours, reaction temperature 50-70℃, then pass the cobalt 60 γ-ray irradiation, cobalt 60 The γ-ray irradiation dose is 5-15kGy. Adjust the pH to 5-7, add 80%-95% ethanol while stirring until the ethanol concentration of the alcohol precipitation system is 50%-80%, let stand overnight at room temperature, take the precipitate, and dry it at 50℃-80℃ for 2-5h, Using a planetary gear ball mill, ball milling for 5-15 hours, a low molecular weight sturgeon chondroitin sulfate product with a molecular weight range of 5000 to 20000 Daltons is obtained.

[0024] It is better to mix chondroitin sulfate into a 5%-15% solution, add oxalic acid to control the oxalic acid concentration in the system to 5%-10% oxalic acid ...

Example Embodiment

[0026] Example 1:

[0027] (1) Preparation of sturgeon spine particles: immerse the sturgeon spine in 90℃ water for 10 minutes, take it out, remove a small amount of residual muscle, fat and other connective tissue on the cartilage, cut into small pieces of 2-5cm length, and wash in water Twice, dry at 60°C for 7 hours, crush, pass through a 20-mesh sieve (particle size≦0.9cm);

[0028] (2) Extract sturgeon chondroitin sulfate: add 7 times the amount of 6% NaOH to the sturgeon spine particles obtained in step (1), stir and extract for 4.5 hours in a water bath at 40°C to obtain sturgeon chondroitin sulfate extract ;

[0029] (3) Enzymatic hydrolysis of sturgeon chondroitin sulfate: adjust the pH of the sturgeon chondroitin sulfate extract obtained in step (2) to 8.5, add a mixed enzyme of trypsin and papain 2.5%, and heat and hydrolyze at 55°C for 5 hours, then take it out Inactivate the enzyme in hot water at 85°C; the mixed mass ratio of trypsin and papain is 1:1;

[0030] (4) Dec...

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Abstract

The invention relates to a method for preparing low-molecular-weight sturgeon chondroitin sulfate by utilizing sturgeon chine. The method is characterized by comprising the following steps of scalding sturgeon chine, removing little residue on cartilage, cutting the sturgeon chine into blocks, and washing with water, drying, pulverizing and filtering the sturgeon chine blocks; adding NaOH and extracting a sturgeon chondroitin sulfate extracting solution in a lukewarm bath; adjusting pH value, adding mixed enzyme of trypsase and papain for enzymolysis, deactivating the enzyme, adjusting pH value, decoloring through active carbon, and filtering; adding 80% to 95% ethanol, drying and pulverizing; degrading through oxalic acid, radiating through 5 to 15kGy cobalt 60gamma ray, adjusting pH value, adding ethanol, extracting the precipitates, drying the precipitates, and carrying out ball milling on the precipitates through a planetary-type gear ball mill, thus obtaining the low-molecular-weight sturgeon chondroitin sulfate with the molecular weight of 5000Dalton to 20000Dalton. The radiation degradation is combined with the supermicro pulverizing technology, so that the molecular weight of the chondroitin sulfate can be reduced, and the bioavailability of the chondroitin sulfate can be improved; moreover, the physical method is simple and easy, the treatment process is pollution-free, the production cost is low, the product purity is high, and the method is suitable for industrialized mass production.

Description

technical field [0001] The invention relates to a method for preparing chondroitin sulfate, in particular to a method for preparing low-molecular-weight sturgeon chondroitin sulfate by using the spine of sturgeon and adopting irradiation degradation and ultrafine pulverization. Background technique [0002] Chondroitin sulfate (CS for short) is a kind of natural acidic mucopolysaccharide from animal cartilage tissue. Studies have shown that CS plays an important role in the prevention and treatment of cardiovascular diseases and joint diseases. It reduces the morbidity and mortality of patients with coronary heart disease, and also has an inhibitory effect on tumors. CS is also an important raw material of popular health products, cosmetics and pharmaceuticals at home and abroad. [0003] With the rapid development of sturgeon farming in my country, my country has now become the world's largest sturgeon farming country. In particular, the cartilage in the head, notochord ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 王俊汪兰程薇王少华熊光权乔宇吴文锦史德芳张金木
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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