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Method for preparing phosphatidyserine

A technology of phosphatidylserine and serine, which is applied in the direction of fermentation, can solve the problems of unsatisfactory conversion rate, high production cost, and excessive phosphatidic acid, and achieve the effect of short cycle, low cost and increased conversion rate

Active Publication Date: 2014-02-05
SHAANXI YUANBANG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a new method for preparing phosphatidylserine, so as to solve the problems in the prior art that the by-product phosphatidic acid is more, the conversion rate is not ideal, and the production cost is high.

Method used

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  • Method for preparing phosphatidyserine
  • Method for preparing phosphatidyserine

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Add 20g of soybean phospholipid with a phosphatidylcholine content of 50.98% in the reaction tank, 40g of L-serine, Tween-800.02g, and 1.6g of calcium chloride. The buffer solution is sodium acetate-acetic acid system, pH value 5.5, buffer solution The addition amount is 100mL, the enzyme activity of phospholipase D is 5000U / g, and the addition amount is 100mg. Stir and keep the reaction temperature at 37°C, the reaction time is 8h, detect the conversion rate, centrifuge and filter, add 20mL of water to the filter residue after centrifugation and wash three times, and centrifugally filter the reaction crude product, add absolute ethanol 100mL to the reaction crude product, soak for 2h, stir for 1h, centrifuge Filtration, vacuum drying to obtain 17.28g PS content is 56.48% powder phospholipid.

Embodiment 2

[0029] Add 50g of phosphatidylcholine content to the reaction tank and be 80.68% sunflower phospholipid, L-serine 150, Tween-800.2g, calcium chloride 6.0g, buffer solution selects sodium acetate-acetic acid system, pH value 5.3, buffer The addition amount of the solution is 400mL, the enzyme activity of phospholipase D is 5000U / g, and the addition amount is 400mg. Stir and keep the reaction temperature at 40°C, the reaction time is 10h, the conversion rate is detected, centrifugal filtration, after centrifugation, the filter residue is washed three times with 50mL of water, and the reaction crude product of centrifugal filtration is added to 400mL of absolute ethanol, soaked for 2h, stirred for 1h, centrifuged Filtration, vacuum drying to obtain 44.88g PS content is 87.75% powder phospholipid.

Embodiment 3

[0031] Adding 50g phosphatidylcholine content is 21.62% soybean lecithin in reaction tank, L-serine 100g, Tween-800.25g, calcium chloride 6.0g, buffer solution selects disodium hydrogen phosphate-phosphoric acid system, pH value 5.1, The added amount of buffer solution is 300mL, the activity of phospholipase D is 5000U / g, and the added amount is 220mg. Stir and keep the reaction temperature at 37°C, the reaction time is 10h, detect the conversion rate, centrifuge and filter, add 50mL water to the filter residue after centrifugation and wash three times, and centrifuge the reaction crude product, add absolute ethanol 300mL to the reaction crude product, soak for 2h, stir for 1h, centrifuge Filtration, vacuum drying to obtain 33.89g PS content is 30.27% powder phospholipid.

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Abstract

The invention relates to a method for preparing phosphatidyserine. The method for preparing phosphatidyserine comprises the following steps of mixing phosphatidylcholine or natural phospholipid containing phosphatidylcholine with tween-80, L-serine, calcium chloride, buffer solution and phospholipase D, and stirring and reacting the above materials at 35-45 DEG C for 8-10h; separating out the crude products from reaction and floating, carrying out centrifugal separation on the aqueous phase and crude products, washing the crude products subjected to separation and carrying out centrifugal filtration; adding the crude products subjected to centrifugal filtration into absolute ethyl alcohol for immersing, stirring, and carrying out centrifugal filtration; and carrying out vacuum drying on the products subjected to centrifugal filtration to obtain the phosphatidyserine. The invention aims to provide a new method for preparing phosphatidyserine to solve the problems that in the prior art, the byproduct of phosphatidic acid is large in amount, the conversion rate is unsatisfactory and the product cost is high.

Description

technical field [0001] The invention relates to a method for preparing stable powdered phosphatidylserine. Background technique [0002] Phospholipid is the abbreviation of phosphoglyceride, which has an important regulatory function on the biological activity of biofilm and the normal metabolism of the body. The so-called phospholipid usually refers to a fatty substance containing phosphorus, which is a mixture of various phospholipids, and its main components are phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), Serine (PS), phosphatidic acid (PA), phosphatidylglycerol (PG), etc. [0003] Phosphatidylserine is the only phospholipid that can regulate the function of key proteins in the cell membrane. It has an important regulatory effect on many cell metabolism processes. Its main function is to improve the vitality of brain cells, which is beneficial to the treatment of brain atrophy, the prevention of Alzheimer's disease, and the improve...

Claims

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Application Information

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IPC IPC(8): C12P13/06
Inventor 李艳赵江张永利寇玉锋
Owner SHAANXI YUANBANG BIO TECH
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