Guava hypoglycemic active component, and preparation method and use thereof

An active ingredient, the technology of guava leaves, applied in the field of preparation, guava hypoglycemic active ingredients, can solve the problems of unspecified guava extract extraction method and specific components, etc., achieve good chemical property stability, reduce high Blood sugar, composition clear effect

Inactive Publication Date: 2014-02-12
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

"A pharmaceutical composition or health product with hypoglycemic effect" (201210505096.8) disclosed in Chinese patent application provides a kind of medicinal composition composed of bitter melon extract, olive extract,...

Method used

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  • Guava hypoglycemic active component, and preparation method and use thereof
  • Guava hypoglycemic active component, and preparation method and use thereof
  • Guava hypoglycemic active component, and preparation method and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Weigh 1.2kg of guava leaf raw material in September, reflux extraction with 10L 50% ethanol for 2 times, each time for 1 hour, concentrate the extract under reduced pressure below 60°C to obtain ethanol extract; disperse the ethanol extract in water, extract with petroleum ether until the color of the solution is When it becomes lighter, replace it with ethyl acetate and extract 6 times until the last extract is very light in color. Combine and concentrate the extract to obtain 35.6g of ethyl acetate extract. The extract is passed through 400g of a 100-mesh polyamide column, washed with water, 10% ethanol was eluted for 4 times the column volume, 50% ethanol was eluted for 8 times the column volume, concentrated to obtain 10.3g solid, and then passed through a 200-mesh polyamide column, 10% ethanol was eluted for 3 times the column volume, and finally 30% ethanol was eluted. Eluted at 10 times the column volume, concentrated, and crystallized with 70% ethanol to obtain 4...

Embodiment 2

[0020] Weigh 20kg of guava leaf raw material in October, reflux extraction with 200L 50% ethanol twice, each time for 2 hours, and concentrate the extract under reduced pressure below 60°C to obtain ethanol extract; disperse the ethanol extract in water, extract with petroleum ether until the color of the solution changes When it is shallow, change to ethyl acetate and extract 10 times until the last extract is very light. Combine and concentrate the extracts to obtain 519.2g of ethyl acetate extract. The extract is passed through a 80-mesh polyamide column, washed with water, and 10% Fully eluted with ethanol, eluted with 50% ethanol for 10 times of column volume, concentrated to obtain 177.8g solid, passed through a 100 mesh polyamide column, eluted with 10% ethanol for 6 times of column volume, and finally eluted with 30% ethanol for isocratic elution, concentrated, 70% ethanol was crystallized to obtain 103.54 g of hypoglycemic active components, which contained 19.72 g of ...

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Abstract

The invention provides a guava hypoglycemic active component, and a preparation method and use thereof. The method comprises the following steps: crushing a raw material of guava leaves; carrying out reflux extraction by 8-10fold by volume of 50% ethanol; carrying out vacuum concentration below 60 DEG C; dispersing the obtained ethanol extract into water; fully extracting by adopting ethyl acetate after petroleum ether is extracted; carrying out vacuum concentration below 60 DEG C; filtering the obtained ethyl acetate extract by a 60-200mesh polyamide chromatographic column; eluting ethanol water from low to high in a gradient manner; collecting a component which is eluted by ethanol of which the concentration is 30-50%, continuing to filter by the 60-200mesh polyamide chromatographic column after vacuum concentration below 60 DEG C; carrying out isocratic elution by 30% ethanol after eluting by 10% ethanol; carrying out vacuum concentration on eluent below 60 DEG C, and recrystallizing by a 70% ethanol solvent, so as to obtain the hypoglycemic active component. The method disclosed by the invention is simple and convenient; chemical components of an effective part are clear in composition, and good in chemical stability. By adopting the guava hypoglycemic active component disclosed by the invention, alloxan-induced hyperglycemia can be significantly reduced, and the guava hypoglycemic active component can be applied to preparation of hypoglycemic medicaments or health-care products.

Description

technical field [0001] The invention relates to the field of hypoglycemic health products, in particular to a guava hypoglycemic active component, preparation method and application. Background technique [0002] Type 2 diabetes is a series of metabolic disorder syndromes caused by a variety of pathogenic factors acting on the body to cause hypofunction of pancreatic islets and insulin resistance. The World Health Organization pointed out that the situation of diabetes worldwide is still very serious, and the situation in developing countries is particularly worrying. [0003] Pomegranate leaves have a long history of being used as folk medicine to prevent and treat diabetes. There are a large number of literature reports on the prevention and treatment of guava leaves in patients with type 2 diabetes, and a large number of clinical trials have proved its curative effect. Therefore, many domestic and foreign Scholars have carried out a lot of research on its hypoglycemic ef...

Claims

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Application Information

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IPC IPC(8): A61K36/61A61P3/10A23L1/30A61K127/00A23L33/105
Inventor 曹庸欧阳文邵祥辉蔡喆俊朱晓艾陈雪香
Owner SOUTH CHINA AGRI UNIV
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