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Edible agar utilized double-coated lactobacillus powder and preparation method thereof

A lactic acid bacteria powder, lactic acid bacteria technology, applied in food preparation, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as insufficient effect and limited application range

Active Publication Date: 2014-02-12
CELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned improved existing lactic acid bacteria coating technology is suitable for the existing powdered lactic acid bacteria products, but in foods with a lot of water content, it cannot be shown to prevent the death of lactic acid bacteria caused by moisture. Therefore, compared with a large number of food types, it is pointed out that there are still the following problems: its application range can only be limited

Method used

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  • Edible agar utilized double-coated lactobacillus powder and preparation method thereof
  • Edible agar utilized double-coated lactobacillus powder and preparation method thereof
  • Edible agar utilized double-coated lactobacillus powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 utilizes the preparation of the double coating lactic acid bacteria powder of edible agar

[0047] First, in order to produce Lactobacillus casei (Lactobacillus casei CBT-LC) lactic acid bacteria coated with protein alone, the following experiment was performed.

[0048] Specifically, 5% glucose was added as a sugar component, 2% yeast extract, 3% soybean peptone, 0.1% edible agar, 0.2% potassium dihydrogen phosphate, 0.2% ammonium citrate, 0.2% sodium acetate, and 0.01% magnesium sulfate were added as The ionic components were then dissolved, transferred to an anaerobic fermentation tube with a capacity of 200L, sterilized at 121°C for 15 minutes, inoculated with 2L of bacteria, maintained pH with ammonia, and fermented for 12 hours. After the above-mentioned fermentation, the fermented liquid was transferred to a continuous centrifugal separator at a flow rate of 60 L / hr to precipitate bacteria and edible agar jelly, thereby forming a concentrated aqueous...

Embodiment 2

[0053] Example 2 Preparation of Lactic Acid Bacteria Double-coated with Proteins and Polysaccharides Containing Edible Agar

[0054] First, the following experiments were conducted in order to produce Bifidobacterium lactis CBT-BL lactic acid bacteria coated with edible agar and protein.

[0055] Specifically, 5% glucose was added as a sugar component, 3% yeast extract, 2% soybean protein isolate as a protein component, 0.3% edible agar, 0.2% potassium dihydrogen phosphate, 0.2% ammonium citrate, 0.2% sodium acetate, 0.01 % magnesium sulfate as ionic components, then dissolve them, transfer to a 200L capacity anaerobic fermentation tube, sterilize at 121°C for 15 minutes, inoculate 2L of bacteria, maintain pH with ammonia, and ferment for 15 hours. After the above-mentioned fermentation, the fermented liquid was transferred to a continuous centrifugal separator at a flow rate of 60 L / hr to precipitate bacteria and edible agar jelly, thereby forming a concentrated aqueous solut...

experiment example 1

[0059] Experimental Example 1 Utilizes the Acid Resistance Comparison of the Double Coated Lactic Acid Bacteria Powder of Edible Agar

[0060] After measuring the acid resistance of the double-coated lactic acid bacteria powder utilizing edible agar prepared in the above-mentioned Example 1 and Example 2, its structure is shown in Table 1.

[0061] React the lactic acid bacteria powder prepared in the above-mentioned Example 1 and Example 2 in the artificial gastric juice of pH 2.1 for 30, 60, 90 and 120 minutes respectively, dilute the water with physiological saline or phosphate-buffer solution (Phosphate-buffer), pass After dilution by the decimal dilution method, spread on a solid medium (agar plate) and count the lactic acid bacteria.

[0062] Table 1

[0063]

[0064] In the above-mentioned Table 1, each control area shows that it is prepared according to the same method as that of Example 1 and Example 2, and forms double-coated lactic acid bacteria powder of protei...

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Abstract

The invention relates to lactobacillus powder, a preparation method thereof, and a product containing the powder. The lactobacillus powder is characterized in that the matrix structure (bacterial block) of double-coated lactobacillus formed by proteins and polysaccharides is solidified by utilizing the edible agar. According to the invention, the double-coated lactobacillus powder including the proteins and polysaccharides of the edible agar has an enhanced acid resistance, an enhanced bile resistance and an enhanced accelerated testing stability, and can be effectively used for preparing a plurality of lactobacillus-containing products.

Description

technical field [0001] The present invention relates to a double-coated lactic acid bacteria powder using edible agar (Agar), more specifically, to a double-coated lactic acid bacteria powder using edible agar and a preparation method thereof. The double-coated lactic acid bacteria powder using edible agar powder, using edible agar to double-coat fermented lactic acid bacteria with protein and polysaccharide components, resulting in a strong matrix (lattice) shape of the bacteria block, thus showing enhanced acid resistance, bile resistance and acceleration stability. Background technique [0002] Lactic acid bacteria, also known as lactic acid bacteria or lactic acid bacteria, inhabit the intestines of mammals and prevent abnormal fermentation caused by miscellaneous bacteria. They are useful bacteria used as digestive aids. For example, Lactobacillus bulgaricus (L. bulgaricus) is the earliest known lactic acid bacterium, which is used to prepare yogurt and is also used as...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23C9/12A23C9/152A23L1/24A23L1/218A23L2/52A23L1/30A61K35/74A61K8/99C12R1/46C12R1/225C12R1/01A23L33/135A61K35/744
Inventor 郑明俊
Owner CELL BIOTECH
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