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Use of C21 steroidal glycoside of root tubers of Cynanchun auriculatum Royle ex Wight, Cynanchum bungei Decne and Cynanchum wifordii Hemsl in activation of tyrosinase

A technology of tyrosinase diphenolase and Baishouwu, which is applied in preparations for dressing, skin care preparations, medical preparations containing active ingredients, etc., to achieve low production cost and strong activation of tyrosinase Effect

Inactive Publication Date: 2014-02-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no C extracted from Baishouwu medicinal materials. 21 Reports on the use of steroidal glycosides in enhancing tyrosinase activity

Method used

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  • Use of C21 steroidal glycoside of root tubers of Cynanchun auriculatum Royle ex Wight, Cynanchum bungei Decne and Cynanchum wifordii Hemsl in activation of tyrosinase
  • Use of C21 steroidal glycoside of root tubers of Cynanchun auriculatum Royle ex Wight, Cynanchum bungei Decne and Cynanchum wifordii Hemsl in activation of tyrosinase
  • Use of C21 steroidal glycoside of root tubers of Cynanchun auriculatum Royle ex Wight, Cynanchum bungei Decne and Cynanchum wifordii Hemsl in activation of tyrosinase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Preparation of total steroidal glycosides of Radix Polygoni Multiflori: Weigh 500 g of Radix Polygoni Multiflori powder, add 1.5 L of 95% ethanol, heat to reflux for 3 hours, and filter to obtain alcohol extract. The extract was concentrated under reduced pressure, and the solvent was evaporated at room temperature to obtain an alcohol extract. Dissolve the extract in a methanol:water (volume ratio) solution of 9:1, shake and degrease 3 times with petroleum ether, add an appropriate amount of distilled water to the raffinate, concentrate under reduced pressure to recover methanol, and perform multiple extractions with chloroform. The chloroform extracts were combined, concentrated to a small volume, then added dropwise to 5 times petroleum ether, stirred to disperse, precipitated, filtered and dried to obtain total steroid glycosides, and a small amount was carried out for Liebermann-Burchard reaction and Keller-Kiliani reaction respectively. Strong positive. Determina...

Embodiment 2

[0017] Silica gel column chromatography of total steroidal glycosides of Radix Polygoni Multiflori: Dissolve the total steroidal glycosides with a small amount of chloroform, pass through silica gel column chromatography, and use chloroform (I), chloroform:methanol (volume ratio) of 98:2 (II), 95 :5(III), 90:10(IV), and methanol (V) for five gradient elutions. Rotate the eluate, evaporate the eluent to dryness, take an appropriate amount of total steroids and each eluent component and dissolve it in a solvent (propylene glycol: ethyl acetate: phosphate buffer volume ratio of 9:1:18) to prepare a sample solution with a concentration of 1 mg / mL.

Embodiment 3

[0019] Preparation of psoralen ethanol extract: Weigh appropriate amount of psoralen powder, psoralen and 50% ethanol at a ratio of 1:6, extract by cold soaking for 3 times, and concentrate the extract and evaporate to dryness. Take an appropriate amount of extract and dissolve it in a solvent to prepare a solution with a concentration of 0.5 mg / mL as a positive control solution.

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PUM

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Abstract

The invention relates to a use of C21 steroidal glycoside (C21 steroidal glycoside A) extracted and separated from root tubers of Cynanchun auriculatum Royle ex Wight, Cynanchum bungei Decne and Cynanchum wifordii Hemsl in activation of tyrosinase. The C21 steroidal glycoside has a strong activation effect on mushroom tyrosinase diphenolase and can be used in the related field. The invention illustrates the novel purpose of the C21 steroidal glycoside in the field of medicine and daily-use chemical industry. The influence produced by the C21 steroidal glycoside A of root tubers of Cynanchun auriculatum Royle ex Wight, Cynanchum bungei Decne and Cynanchum wifordii Hemsl on the vitality of the tyrosinase diphenolase is detected through an enzymatic reaction, and the result shows that the C21 steroidal glycoside A has strong effects of activation of mushroom tyrosinase diphenolase and has a tyrosinase activation rate of 293.28% based on the final concentration of 0.05mg / ml, and the C21 steroidal glycoside A contains different active components having competitive and non-competitive dual activation effects on tyrosinase and has a mixed activation type. The C21 steroidal glycoside A of root tubers of Cynanchun auriculatum Royle ex Wight, Cynanchum bungei Decne and Cynanchum wifordii Hemsl can be used for preparation of drugs for treating pigment deletion-caused diseases and can also be used for preparation of daily chemical articles for blacking skin and hair.

Description

technical field [0001] The present invention relates to a kind of C in Baishouwu 21 Use of steroidal glycosides for tyrosinase activation. The invention relates to a C 21 The steroidal glycoside has a strong activation effect on tyrosinase, and its preparation method and its new application in the fields of medicine and daily chemical industry are clarified. Background technique [0002] Tyrosinase is a copper-containing polyphenol oxidase, which is the rate-limiting enzyme in the biosynthesis of melanin, catalyzing the hydroxylation of tyrosine to dopa, the oxidation of dopa to dopaquinone and the 5,6 - Oxidation of dihydroxyindole (DHI) to 5,6-indoloquinone in three steps. Its expression and activity determine the amount and speed of melanin production. Excessive reaction can cause hypermelanin, such as freckles, chloasma, etc. Excessive melanin production is the root cause of chloasma; too weak or lack of reaction can cause white hair, vitiligo, etc. The activity of ...

Claims

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Application Information

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IPC IPC(8): A61K36/27A61K8/97A61K8/63A61Q19/04A61Q5/00A61P17/00A61K125/00
Inventor 王晓岚姚文杰苑亮
Owner JIANGNAN UNIV
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