Specific PCR (polymerase chain reaction) method for rapidly identifying common high temperature actinomyces

Abstract

The invention discloses a specific PCR (polymerase chain reaction) method for rapidly identifying common high temperature actinomyces. According to the technical scheme, genome DNA (deoxyribonucleic acid) of a sample is taken as a template, a specific primer designed by the invention is adopted for PCR so as to amplify housekeeping gene of the characteristic of the common high temperature actinomyces, an actual amplified fragment is 733bp, and amplified products are detected by agarose gel electrophoresis so as to identify whether the sample is the common high temperature actinomyces. The specific PCR method can be used for identifying the common high temperature actinomyces rapidly only through three steps of DNA extraction, PCR amplification and electrophoresis detection, and has remarkable advantages of being efficient, sensitive, convenient and fast and low in cost.

Description

technical field [0001] The invention belongs to the field of molecular biology identification, and in particular relates to a rapid identification method for common high-temperature actinomycetes. Background technique [0002] common high temperature actinomycetes ( Thermoactinomyces vulgaris ) was first discovered by Tsiklinsky in 1899 and is Eubacteria - Firmicutes - Bacilli - Bacillales - Thermoactinomycetaceae - Thermoactinomycetes A typical species of the genus Thermoactinomyces, which is commonly found in high-temperature environments such as high-temperature composting. It is thermophilic and can produce abundant hyphae and endospores. [0003] Ordinary thermoactinomycetes are very rich in enzyme production, and can produce heat-resistant α-amylase, protease, lipase, DNA polymerase, carboxypeptidase T and chitinase, which can decompose starch, pullulan in high temperature environment Sugar, cyclodextrin, elastin, collagen, fat and other substrates have important app...

AI Technical Summary

Problems solved by technology

However, the morphological characteristics, physiological and biochemical characteristics, and 16S rRNA gene sequence are very similar between common thermoactinomycetes and their close relatives. The identification by conventional techniques is cumbersome and time-consuming, and cannot satisfy the screening and application of common thermoactinomycetes. Therefore, there is an urgent need to establish a set of accurate and rapid identification methods for ordinary high-temperature actinomycetes, so as to provide technical support for the breeding of strains and their application research.

Images