Built-in disc fibrous bed reactor for fermenting production of butyric acid

A reactor and fiber bed technology is applied in the field of built-in disc fiber bed reactors, which can solve the problems of small immobilization area of ​​cotton fibers, low utilization rate of fermentation equipment, and high probability of bacterial contamination, so as to solve the difficulties of disinfection and sterilization, The effect of low immobilization cost and large surface area

Inactive Publication Date: 2014-03-19
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the small cotton fiber immobilization area and uneven mass transfer in the existing built-in cotton fiber reactor, which affects its wide application, and the high probability of bacterial contamination in the fermentation process of the external fiber bed reactor and the utilization of fermentation equipment Due to the shortcomings of low efficiency, the present invention provides a built-in dish fiber bed reactor for the production of butyric acid by fermentation with high mass transfer efficiency and equipment utilization rate and low probability of bacterial contamination in the fermentation process

Method used

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  • Built-in disc fibrous bed reactor for fermenting production of butyric acid
  • Built-in disc fibrous bed reactor for fermenting production of butyric acid
  • Built-in disc fibrous bed reactor for fermenting production of butyric acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 produces the method for butyric acid

[0039] Butyric acid was produced in 100 mL anaerobic serum bottles using Clostridium tyrobutyricum ATCC25755. Both the seed and fermentation medium formulations are: 30g / L glucose, 5g / L yeast extract (BBI), 5g / L peptone (BBI), 6g / L NaCl, 3g / L (NH 4 ) 2 · SO 4 , 1.5g / L K 2 HPO 4 , 0.6g / L MgSO 4 ·7H 2 O, 0.03g / LFeSO 4 ·7H 2 O, 0.3g / L cysteine-HCl, 0.05mL resazurin indicator solution (1g / L), boiled in a boiling water bath to remove oxygen, carbon source and nitrogen source were sterilized separately, pH6.0.

[0040] Put 3ml Clostridium tyrobutyricum seed solution into 60ml fermentation medium and different immobilized fiber materials cut into 2mm*2Mm size (hemp fiber fabric, cotton fiber fabric, bamboo fiber fabric, wool fiber fabric, synthetic fiber such as polyester fabric, acrylic fabric) in anaerobic serum bottles (total volume: 100ml, initial pH: 6.0), cultured at 37°C until the end of fermentation, see Tab...

Embodiment 2

[0044] Embodiment 2 produces the method for butyric acid

[0045] Clostridium tyrobutyricum (Clostridium tyrobutyricum ATCC25755) was used to produce butyric acid in a built-in dish fiber bed reactor.

[0046] Seed medium: 30g / L glucose, 5g / L yeast extract (BBI), 5g / L peptone (BBI), 6g / L NaCl, 3g / L (NH 4 ) 2 · SO 4 , 1.5g / L K 2 HPO 4 , 0.6g / LMgSO 4 ·7H 2 O, 0.03g / L FeSO 4 ·7H 2 O, 0.3g / L cysteine-HCl, 0.05mL resazurin indicator solution (1g / L), boiled in a boiling water bath to remove oxygen, carbon source and nitrogen source were sterilized separately, pH6.0.

[0047] Fermentation medium: 60g / L glucose, 5g / L yeast extract (BBI), 5g / L peptone (BBI), 6g / L NaCl, 3g / L (NH 4 ) 2 · SO 4 , 1.5g / L K 2 HPO 4 , 0.6g / LMgSO 4 ·7H 2 O, 0.03g / L FeSO 4 ·7H 2 O, carbon source and nitrogen source are sterilized separately, pH 6.0.

[0048] Fix the composite disc-shaped fiber structure of different sizes in the reactor body, insert 150ml Clostridium tyrobutyricum seed liquid ...

Embodiment 3

[0052] Embodiment 3 produces the method for butyric acid

[0053] Clostridium tyrobutyricum (Clostridium tyrobutyricum ATCC25755) was used to produce butyric acid in a built-in dish fiber bed reactor.

[0054] Wherein seed culture medium and fermentation medium are identical with embodiment 2.

[0055] Different numbers of composite disc fiber structures (diameter ratio of 0.8) were fixed in the reactor body, and 150ml Clostridium tyrobutyricum seed liquid was inserted into the reactor body containing 3L seed medium (total volume: 5L ), the temperature is 37°C, and N 2 Maintain the anaerobic state of the tank, stir with a stirring device (speed 160r / min), and at the same time use the first constant flow pump to add 8mol / L sodium hydroxide solution to control the pH of the seed liquid in the reactor body to 6.0, and cultivate for 30 hours to achieve Cells are immobilized on composite discs. Replace the seed solution in the reactor body with the fermentation medium, keep the ...

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Abstract

The invention discloses a built-in disc fibrous bed reactor for fermenting production of butyric acid. The reactor comprises a reactor body, a stirring device, a pH automatic control device and a material supplying device, and further comprises a composite disc type fibrous structures which are fixedly arranged in the reactor body at intervals as cell immobilization carriers, wherein the pH automatic control device and the material supplying device are respectively connected to the reactor body; the stirring device is arranged in the reactor body; the stirring device is free from being in contact with the composite disc type fibrous structures; each composite disc type fibrous structure comprises a disc type stainless steel net and immobilization fibrous materials; the immobilization fibrous materials cover both upper and lower surfaces of the disc type stainless steel nets. Equipment utilization rate of the built-in disc fibrous bed reactor is high and the immobilization fibrous materials are cheap; the reactor is high in acid production strength and stable in repeated batch production when used for producing the butyric acid, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a built-in dish fiber bed reactor for fermenting and producing butyric acid, which belongs to the technical field of fermentation engineering. Background technique [0002] Butyric acid and its derivatives are important organic chemical raw materials, widely used in food, chemical, pharmaceutical, energy and other fields. In the field of food industry, pure butyric acid can increase the creamy taste of edible spices, and can also be used as a pH regulator and bacterial inhibitor in food additives; as a feed additive to replace antibiotics, butyrate can promote animal growth, regulate Intestinal microorganisms in animals, and the function of increasing the immunity of the body; in the chemical industry, butyric acid can be used to manufacture fiber plastics with good properties such as cellulose butyrate and cellulose acetate butyrate; it is also reported that butyric acid can be used as a new A precursor to butanol, a first-ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/04C12M1/02
CPCC12M21/12C12M25/02C12M27/02C12M29/00C12M41/26
Inventor 黄金代洪亮王普李军严人
Owner ZHEJIANG UNIV OF TECH
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