Preparation method of reagent for changing glucose hydroxyl configuration
A glucose and glucofuranose technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of wasting oxidants, difficult to separate products, and low purity of oxidation products
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Embodiment 1
[0014] Embodiment 1: the preparation of oxidizing agent pyridinium dichromate (PDC)
[0015] Add 50ml of water and 50g of chromium trioxide into a 500ml four-necked bottle equipped with a thermometer and mechanical stirring, and add 40.3ml of pyridine dropwise to the reaction system while maintaining the temperature at 15-25°C. Then slowly add 200ml of acetone to the reaction liquid, filter the mixture after ice-cooling, and dry to obtain the oxidizing agent pyridinium dichromate (PDC).
Embodiment 2
[0016] Example 2: Oxidation of diisopropylidene-α-D-glucofuranose
[0017] Add 350ml of dry dichloromethane, 40.6g of pyridinium dichromate and 44.1g of acetic anhydride into a 1000ml four-neck flask equipped with a thermometer, condenser and mechanical stirring, and add di The solution of isopropylidene-α-D-glucofuranose (31.2g of diisopropylidene-α-D-glucofuranose+50ml of dry dichloromethane) was heated under reflux for 2h after the dropwise addition was completed. Then concentrate under reduced pressure (no more than 65°C), extract the concentrate with 200ml×2 ethyl acetate, filter the extract and residue through a column (100ml100-200 mesh silica gel, 10ml anhydrous magnesium sulfate liner on the head of the column), and use 600ml of ethyl acetate was used for elution, and the combined eluents were the oxidation products.
Embodiment 3
[0018] Example 3: Synthesis and Purification of Diisopropylidene-α-D-Allofuranose
[0019] The eluate in Example 2 was concentrated under reduced pressure (not exceeding 65° C.), and the concentrate was dissolved in ethanol solution (80 ml ethanol + 60 ml water). Maintain the temperature at 20-25°C under stirring, add sodium borohydride solution (5.6g sodium borohydride + 80ml water) dropwise to the solution within 0.5h, and react at room temperature for 3h. The reaction solution was extracted with 200ml×3 dichloromethane respectively, the organic layers were combined, washed with 100ml of water, and then dried with 100g of anhydrous magnesium sulfate overnight. Filtrate, concentrate the filtrate, and recrystallize the resulting solid with ether to obtain white crystals, which are the pure product of diisopropylidene-α-D-allofuranose.
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