Oryza glaberrima gene introgression restoration line and partial interspecies cross paddy rice breeding method
A technology of gene introgression and hybrid rice, which is applied in the fields of botanical equipment and methods, plant gene improvement, and application to achieve high yield and stress resistance, and improve the utilization level.
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Embodiment 1
[0054] A method for selecting and breeding African cultivated rice gene introgression restorer lines, the steps of which are:
[0055] 1) Interspecific hybridization between African cultivated rice and common cultivated rice:
[0056] 19 strains of African cultivated rice RAM3, RAM48, RAM54, RAM111, RAM131, RAM152, etc., which were headed under short-day conditions in spring in Hainan, were used as female parents. Detasseling in warm soup: water temperature 44 to 46°C, treatment time 5 to 6 minutes, with 138 The male parents of indica rice varieties were artificially saturated and pollinated, and 1,200 pairs of hybrids were mated, with 5 hybrid bags per bag and 2-3 panicles in each bag. Seeds were obtained from 22 combinations in which 9 African cultivated rice materials were crossed with 16 indica rice varieties. Between F 1 Generation seeds, the interspecific F1 hybrids harvested in this example are all shrunken and shriveled seeds that are not fully developed.
[0057] 2)...
Embodiment 2
[0067] A method for selecting and breeding partly interspecific hybrid rice combinations, the steps of which are:
[0068] 1) The African rice introgression restorer line obtained by screening with high general combining ability is used as the male backbone line, and the rice cytoplasmic male sterile line or photothermo-sensitive sterile line material is used as the female parent to carry out hybridization testing and mating to obtain some of its species. inter F1 hybrid;
[0069]2) For the F1 planting plot, with the popularized varieties as the control, the pollen fertility was inspected under the microscope at the heading stage, and the relevant agronomic traits were investigated at the maturity stage, and the seeds were harvested separately for test and yield calculation; some interspecific hybrids with good agronomic performance and strong yield advantages were screened Combination for retest.
[0070] 3) Repeat step 1 and step 2, plant and identify the strong and superio...
Embodiment 3
[0076] Molecular validation of African rice introgression lines by SSR markers:
[0077] According to the published rice microsatellite primer sequences (http: / / www.gramene.org / ), 53 pairs of SSR primers distributed on 12 rice chromosomes were selected. PCR amplification and polymorphism analysis were carried out on the introgression lines of Oryza sativa tested, and the donor materials of Oryza sativa and Oryza sativa involved in the breeding process.
[0078] The positions, allele numbers and PIC values of the 53 pairs of SSR primers used are as follows:
[0079]
[0080] 1) DNA extraction of related rice materials:
[0081] Genomic total DNA was extracted from O. sativa introgression lines, O. sativa and Oryza sativa involved in the breeding process, and the conventional CTAB DNA extraction method was used. Refer to the CTAB extraction method of McCouch et al. (2002) improved by Zhang Tao et al. (2007):
[0082] (1) Take 0.1-0.2g of fresh tender leaves and place the...
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