Preparation method of high-activity purple sweet potato dietary fiber
A technology of dietary fiber and purple sweet potato, which is applied in food preparation, food science, application, etc., can solve the problems of low content of soluble dietary fiber, low physiological activity and application value, and achieve good water absorption, expansibility and high physiological activity Effect
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Embodiment 1
[0020]Example 1: Utilize purple sweet potato pigment waste residue powder to prepare highly active purple sweet potato dietary fiber
[0021] Rinse 500g of purple sweet potato pigment production waste residue powder with clean water twice, add about 4kg of clean water, and stir evenly. Use phosphate buffer to adjust the pH of the feed solution to 6.0, add 5×10 3 Uα-amylase, hydrolyze at 60°C for 50 minutes, heat to inactivate the enzyme; adjust the pH to 4.0 with acetic acid, add 7.5×10 4 U-glucoamylase, heat at 65°C for 60 minutes for enzymatic hydrolysis. Heat to inactivate the enzyme; adjust the pH to 7.0 with sodium hydroxide, add 3.5×10 4 U Neutral protease, enzymolysis at 40°C for 90 minutes, heating to inactivate the enzyme; the above-treated feed solution was centrifuged at 1000 rpm for 10 minutes in a desktop centrifuge, and the precipitate and supernatant were separated. Add 16kg of ethanol solution with a mass percentage concentration of 95% to the supernatant, l...
Embodiment 2
[0024] Embodiment 2: directly utilize the purple sweet potato pigment production waste residue that factory produces newly to prepare highly active purple sweet potato dietary fiber
[0025] 50kg of purple sweet potato pigment production waste residues were pulverized and passed through a 100-mesh sieve. Rinse twice with clean water, add about 300kg of clean water, and stir well. Adjust the pH of the feed solution to 6.5, add 5×10 5 Uα-amylase, hydrolyze at 60°C for 50 minutes, heat to inactivate the enzyme; adjust the pH to 4.5 with acetic acid, add 7.5×10 6 U glucoamylase, heat at 60°C for 65 minutes for enzymatic hydrolysis. Heat to inactivate the enzyme; adjust the pH to 7.5 with sodium hydroxide, add 3.5×10 6 U neutral protease, enzymolysis at 45 DEG C for 85 minutes, heating to inactivate the enzyme; the above-treated feed liquid was centrifuged at 1000 rpm for 10 minutes through a three-legged centrifuge, and the supernatant was added with 1200kg mass percent ...
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