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Fluorescence analysis method for determination of glucose by nano-copper oxide mimicking peroxidase

A technology of nano-copper oxide and peroxidase, which is applied in the field of analytical chemistry and nanometers, can solve problems such as interference, poor selectivity, and false increase in blood sugar measurement values, and achieve low cost, small sample demand, and good reproducibility Effect

Inactive Publication Date: 2016-03-30
FUJIAN MEDICAL UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Commercial blood glucose meters are mostly completed in one step, but the selectivity is poor, and they are seriously interfered by substances such as maltose, xylose, and galactose, which often lead to a false increase in the blood glucose measurement value; the glucose oxidase-peroxidase method The operation is simple, the source of reagents is wide and the price is cheap, and it is the blood glucose determination method recommended by the Ministry of Health of my country

Method used

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  • Fluorescence analysis method for determination of glucose by nano-copper oxide mimicking peroxidase
  • Fluorescence analysis method for determination of glucose by nano-copper oxide mimicking peroxidase
  • Fluorescence analysis method for determination of glucose by nano-copper oxide mimicking peroxidase

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Experimental program
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Effect test

example 1

[0038] The specific preparation steps of nano-copper oxide are as follows: (1) Take 150ml of 0.02mol / L copper acetate solution and 0.5ml of glacial acetic acid into a three-necked bottle equipped with a condenser, stir and heat to boiling; (2) Quickly add 0.04g 10ml of sodium hydroxide solution per ml, continue to stir for 5 minutes after adding, and obtain a brown copper oxide precipitate; (3) Centrifuge the black copper oxide precipitate obtained from the reaction, wash it with absolute ethanol three times, and dry it under reduced pressure to obtain the diameter It is 6nm nano copper oxide powder.

example 2

[0040] 0.8mL concentration is 18.75mmol / L terephthalic acid, 0.5mL concentration is 1mol / L hydrogen peroxide and 50μL concentration is that the nano-copper oxide prepared by Example 1 of 40mg / L is added to 3.65mL concentration and is 200mmol / L In phosphate buffers of different pH (pH3~10), mix and shake well and place in a warm bath at 45°C, and measure the fluorescence intensity at 421nm after 20 minutes (excitation wavelength is 315nm). Such as figure 1As shown, the fluorescence intensity reached a maximum at pH 7.0.

example 3

[0042] 0.8mL concentration is 18.75mmol / L terephthalic acid, 0.5mL concentration is 1mol / L hydrogen peroxide and 50μL concentration is that the nano-copper oxide prepared by Example 1 of 40mg / L is added to 3.65mL concentration and is 200mmol / L phosphate buffer (pH 7.0), mixed and shaken, placed in different temperature (20 ~ 55 ℃) warm bath, after 20 minutes to measure its fluorescence intensity at 421nm (excitation wavelength is 315nm). Such as figure 2 As shown, the fluorescence intensity reaches a maximum at 45°C.

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Abstract

The invention discloses a Fluorescence analysis method of measuring glucose by nano-copper oxide simulating peroxidase , the nano-copper oxide is a fluorescent analysis method for simulating peroxidase to measure glucose, which is characterized in that glucose, glucose oxidase and phosphate buffer are mixed in an incubating bath, and then phosphate buffer is added thereto. Phthalic acid and nano-copper oxide continue the warm bath. The fluorescence excitation wavelength and emission wavelength of the reaction product of the above mixed solution are 315nm and 421nm respectively. The linear range of glucose content determination is 3~120μmol / L, and the detection limit is 1.94μmol / L. This method can be used for the determination of blood glucose concentration.

Description

technical field [0001] The invention relates to a fluorescence analysis method for measuring glucose by simulating peroxidase using nanometer copper oxide, which belongs to the field of analytical chemistry and nanotechnology. Background technique [0002] At present, there are o-toluidine method and enzymatic method for clinical determination of blood glucose. The o-toluidine method is simple in operation and low in cost, but has poor specificity, and the o-toluidine reagent is unstable and highly toxic. The enzymatic method is an analysis method that utilizes the highly selective catalysis of enzymes. According to different enzyme types, enzymatic methods can be divided into hexokinase method, glucose dehydrogenase method and glucose oxidase-peroxidase method. Commercial blood glucose meters are mostly completed in one step, but the selectivity is poor, and they are seriously interfered by substances such as maltose, xylose, and galactose, which often lead to a false inc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 陈伟胡爱玲何少斌邓豪华李光文
Owner FUJIAN MEDICAL UNIV
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