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Antioxidant extract of Phanerochaete chrysosporium and preparation method thereof

An anti-oxidant technology of Phoenicia protozoa, applied in the directions of antitoxins, fungi-derived medical raw materials, drug combinations, etc., can solve the problems of excessive free radical production, cell damage, and disorder of the free radical scavenging system, so as to maintain oxidation The effect of restoring balance, inhibiting lipid peroxidation, and unique affinity

Inactive Publication Date: 2017-01-04
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under adversity, such as heavy metal stress, on the one hand, there are too many free radicals, and on the other hand, the free radical scavenging system will be disordered, which will damage the cells.

Method used

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  • Antioxidant extract of Phanerochaete chrysosporium and preparation method thereof
  • Antioxidant extract of Phanerochaete chrysosporium and preparation method thereof
  • Antioxidant extract of Phanerochaete chrysosporium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] An antioxidant extract of Phanerochaete chrysosporium, which uses Phanerochaete chrysosporium cells as the extract, and the Phanerochaete chrysosporium is Phanerochaete chrysosporium BKMF-1767, and the preservation number is CCTCC AF96007, preserved in the China Typical Culture Collection Center; using ultrapure water as the extractant, mixing Phanerochaete chrysosporium cells with ultrapure water, grinding, and centrifuging to obtain Phanerochaete chrysosporium antioxidant extract , each 10 mL of Phanerochaete chrysosporium antioxidant extract contains 0.16 μmol of glutathione, 0.89 μmol of flavonoids and 0.15 μmol of polyphenols.

[0030] The preparation method comprises the following steps:

[0031] (1) Under sterile conditions, inoculate 2ml of Phanerochaete chrysosporium spore suspension in 100ml Kirk medium, and culture at 30-35°C for 5 days.

[0032] The specific composition of Kirk medium is, KH 2 PO 4 2.0 g, MgSO 4 ·7H 2 O 0.71 g, VB 1 (Vitamin B1) 0.01 ...

Embodiment 2

[0037] An antioxidant extract of Phanerochaete chrysosporium, which uses Phanerochaete chrysosporium cells as the extract, and the Phanerochaete chrysosporium is Phanerochaete chrysosporium BKMF-1767, and the preservation number is CCTCC AF96007, preserved in the China Typical Culture Collection Center; using ultrapure water as the extractant, mixing Phanerochaete chrysosporium cells with ultrapure water, grinding, and centrifuging to obtain Phanerochaete chrysosporium antioxidant extract , each 10ml of Phanerochaete chrysosporium antioxidant extract contains 0.67 μmol of glutathione, 0.58 μmol of flavonoids and 0.54 μmol of polyphenols.

[0038] The preparation method comprises the following steps:

[0039] (1) Under sterile conditions, inoculate 2ml of Phanerochaete chrysosporium spore suspension in 100ml Kirk medium, and culture at 30-35°C for 3 days.

[0040] (2) Collect the Phanerochaete chrysosporium balls, wash them with sterile ultrapure water for 3 to 5 times, filter...

Embodiment 3

[0043] Determination of glutathione

[0044] Take 0.25 mL of Phanerochaete chrysosporium antioxidant extract, add 0.25 mol / L, 0.5 mL of Tris-HCl buffer solution with a pH of 8.0, shake well, then add 0.25 mL of formaldehyde solution with a mass concentration of 3%, and shake well After standing at room temperature for 20 minutes accurately, immediately add 3 mL of 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) reagent (1 mM) in a water bath at 25 °C, shake well, and accurately After standing for 5 min, replace the DTNB reagent with phosphate buffer as a blank, shake well, immediately measure the absorbance at a wavelength of 412 nm, and calculate the GSH content according to the GSH standard curve.

[0045] The results of the glutathione assay were as follows: figure 1 As shown, ultrapure water can effectively extract glutathione from Phanerochaete chrysosporium cells, and when the concentration of Phanerochaete chrysosporium cells is 10 mg / ml, the antioxidant extraction The GSH ...

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Abstract

The invention relates to the technical field of antioxidant compositions, and particularly relates to a Phanerochaete chrysosporium antioxidant extractive solution which is prepared by mixing Phanerochaete chrysosporium with ultrapure water, grinding, centrifuging and separating. Each 10 mL of Phanerochaete chrysosporium antioxidant extractive solution contains 0.1-0.7 mu mol of glutathione, 0.5-1 mu mol of flavonoids and 0.1-0.6 mu mol of polyphenols. The Phanerochaete chrysosporium antioxidant extractive solution has high antioxidant activity, and has a synergetic effect. The invention also provides a preparation method of the Phanerochaete chrysosporium antioxidant extractive solution, and the method comprises the following steps: weighing Phanerochaete chrysosporium strain, transferring to a glass homogenizer, adding ultrapure water, grinding, centrifuging, and collecting supernatant; adding ultrapure water to the glass homogenizer, grinding, centrifuging, and collecting supernatant; mixing supernatants, and adding ultrapure water to a constant volume. The preparation method provided by the invention is simple.

Description

technical field [0001] The invention relates to the technical field of antioxidant compositions, in particular to an antioxidant extract of Phanerochaete chrysosporium and a preparation method thereof. Background technique [0002] Active oxygen is an inevitable product of microbial growth and metabolism, and the most common active oxygen in cells includes superoxide radicals (O 2 ·- ), hydrogen peroxide (H 2 o 2 ) and hydroxyl radicals (·OH). Under normal circumstances, low levels of reactive oxygen species (ROS) are necessary for cells to perform biological functions, because ROS can participate in the regulation of signal transduction in many important pathways in cells. However, in some special cases, such as the redox metabolism disorder of the cell itself or external ion radiation, etc., a large amount of ROS will accumulate in the cell. Due to the high activity and strong oxidation reaction ability of reactive oxygen species, they can attack any molecule they enc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/06A61P39/06
Inventor 许飘陈明曾光明黄丹莲刘亮赵美花赖萃张辰王聪冷阳程敏
Owner HUNAN UNIV
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