Panerochaete chrysosporium extracting solution with antioxidant enzyme and preparation method

Abstract

The invention relates to a panerochaete chrysosporium extracting solution with antioxidant enzyme. The panerochaete chrysosporium extracting solution with antioxidant enzyme is prepared by taking a panerochaete chrysosporium bacterium as an extracted substance, a phosphate buffer as an extracting agent, mixing up the panerochaete chrysosporium bacterium with the phosphate buffer, grinding and performing eccentric separation. The panerochaete chrysosporium extracting solution with antioxidant enzyme comprises superoxide dismutase, glutathione peroxidase and catalase. The panerochaete chrysosporium extracting solution is high in oxidation resistance. The invention further relates to a preparation method. The preparation method comprises the following steps: weighing the panerochaete chrysosporium bacterium, transferring into a glass homogenizer, adding the phosphate buffer, grinding, centrifuging to obtain the supernate, further adding the phosphate buffer into the glass homogenizer, grinding, centrifuging to obtain the supernate, and adding the phosphate buffer into the supernate until the constant volume is achieved. The panerochaete chrysosporium extracting solution is simple in process.

Description

technical field [0001] The invention relates to the technical field of antioxidant compositions, in particular to an extract of Phanerochaete chrysosporium containing antioxidant enzymes and a preparation method. Background technique [0002] Active oxygen is an inevitable product of microbial growth and metabolism, and the most common active oxygen in cells includes superoxide radicals (O 2 ·- ), hydrogen peroxide (H 2 o 2 ) and hydroxyl radicals (·OH). Under normal circumstances, low levels of reactive oxygen species (ROS) are necessary for cells to perform biological functions, because ROS can participate in the regulation of signal transduction in many important pathways in cells. However, in some special cases, such as the redox metabolism disorder of the cell itself or external ion radiation, etc., a large amount of ROS will accumulate in the cell. Due to the high activity of free radicals and strong oxidation reaction ability, they can attack any molecule they en...

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Problems solved by technology

However, under adversity, such as heavy metal stress, on the one hand, there are too many free radicals, and on the other hand, the free radical scavenging system will be disordered, which will damage the cells.

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