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A liquid kit and detection method for acid phosphatase detection

A technology of acid phosphatase and kit, applied in the field of biochemical analysis, which can solve the problems of poor stability of liquid reagents, errors in adding samples to reconstituted water, and increased testing costs, and achieve the effects of simple reagents, uniform components, and reduced testing costs

Active Publication Date: 2017-12-19
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method adopts a continuous detection method, and the operation is relatively simple and convenient, but the α-naphthyl phosphoric acid and the diazonium reagent in the reagent have poor stability and are easily hydrolyzed in the buffer solution of about pH5.0, the optimum pH value of ACP, resulting in liquid Poor reagent stability
Although most manufacturers have launched dry powder kits by freeze-drying to solve the problems of storage and transportation of finished products, they are still affected by the stability after reconstitution and the bottle gap caused by the addition error of reconstitution water during use. bad influence
Moreover, in use, if the packaging is too large, it will cause waste after the stable period after reconstitution; if the packaging is too small, the price itself will be high, which will increase the cost of testing

Method used

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  • A liquid kit and detection method for acid phosphatase detection
  • A liquid kit and detection method for acid phosphatase detection
  • A liquid kit and detection method for acid phosphatase detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Prepare ACP Quantitative Detection Kit A according to the following formula, including matrix solution and chromogenic solution:

[0042] Matrix fluid

α-naphthyl phosphate

5.283g

Glycine

5.63g

trisodium citrate

29.41g

Triton X-100

1mL

Sodium azide

1g

Adjust pH to 10.0 with NaOH

Set volume to 1L

[0043] Chromogenic solution

4-Chloro-2-methylbenzenediazide salt

5.144g

Glycine

5.63g

citric acid

21.01g

Triton X-100

1mL

Sodium dodecyl sulfate (SDS)

0.5g

Adjust pH to 1.2 with HCl

Set volume to 1L

[0044] Mix the base solution and the chromogenic solution at a ratio of 2:1 to obtain the working solution, and the pH of the working solution is 4.8.

[0045] Set the parameters of the automatic biochemical analyzer: the analysis method is kinetic method, the main wavelength is 412nm, the secondary wavelength is 70...

Embodiment 2

[0057] Weigh an appropriate amount of pure acid phosphatase dry powder, dilute with double distilled water, prepare standard solutions with ACP activities of 30U / L, 60U / L, 90U / L, and 120U / L, and use the above kit A to repeat the test 3 times per sample. times, calculate its mean (y). Concrete test method is with embodiment 1.

[0058] Take the concentration of the standard solution as the abscissa (x), and the mean value of the measurement results as the ordinate (y) for linear regression, and calculate the linear regression correlation coefficient r. The linear regression correlation coefficient is required to be r≥0.99, and at the same time, substitute the standard solution concentration (x) into the linear Regression equation, calculate the linear regression value (y1), and calculate the relative deviation or absolute deviation (S) of each measured average value (y) and linear regression value (y1) according to the following formula.

[0059] Absolute Deviation = (y-y1)

...

Embodiment 3

[0063] Take a number of kits prepared above, and store them at 4°C and room temperature (25°C), and open the sealed reagents regularly for appearance and properties, deviation measurement of quality control products, and linear range testing, and investigate the performance of the reagents at 4°C and room temperature. Stability, the results are shown in Table 2.

[0064] Concrete test method is with embodiment 1.

[0065] The results showed that the appearance of the reagents did not change after 0, 1, and 2 months of storage at room temperature, and they were all colorless and transparent liquids, and the pH value remained unchanged; On the 60th day, the linear range was 0-100U / L. Stored at 4°C, measured on the 0th, 2nd, 4th, 6th, 8th, 10th, and 12th months, the appearance is colorless and transparent liquid, and the pH value basically does not change; the quality control deviation is less than 10%; 4°C for 12 months, linear range 0-100U / L. It shows that the liquid ACP kit...

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Abstract

The invention discloses a liquid reagent kit for acid phosphatase detection, which comprises a matrix liquid containing a substrate of acid phosphatase, and a color developing liquid containing a color developing agent which has a color reaction to a product reacted with the substrate , matrix solution and chromogenic solution are stored separately in their appropriate pH conditions, and mixed in proportion to obtain a working solution when used. The liquid kit is convenient to use and has good stability.

Description

technical field [0001] The present invention relates to the field of biochemical analysis. More specifically, the present invention relates to a liquid kit for detecting acid phosphatase in biological samples, and a method for detecting acid phosphatase activity using the kit. Background technique [0002] Acid phosphatase (ACP) is a hydrolase that catalyzes the hydrolysis of phosphate monoesters to generate inorganic phosphate under acidic conditions. It exists in various human organs such as the prostate, liver, spleen and blood cells, but the prostate is the most abundant. When these tissue cells are damaged, ACP is released into plasma. Therefore, detecting the content of ACP in human body fluids, especially serum or plasma, is a common biochemical testing item. [0003] At present, the in vitro diagnostic kits used to detect ACP on the market mainly use α-naphthyl phosphate as a substrate. Under acidic conditions, serum ACP catalyzes the hydrolysis of α-naphthyl phosp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 杜少卿蔡锦刚唐玮
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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