Test method for pulse-activating preparation adopting pilose asiabell root

A technology of Shengmai and preparations, which is applied in the content determination of Shengmai preparations (Dangshenfang) and the content determination of traditional Chinese medicine compound preparations. Improve the level of quality evaluation and quality control, save testing time and experimental costs, the effect of large workload and inspection costs

Active Publication Date: 2014-05-28
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods only measure the content of a single component in this group of Chinese patent medicines, so the evaluation of the qua

Method used

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  • Test method for pulse-activating preparation adopting pilose asiabell root
  • Test method for pulse-activating preparation adopting pilose asiabell root
  • Test method for pulse-activating preparation adopting pilose asiabell root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Optimization of chromatographic conditions (taking Shengmaiyin (Dangshen Fang) as an example)

[0045] 1. Selection of detection wavelength

[0046] The liquid chromatograms ( figure 1 ), the preparation method of the test solution is: accurately pipette 920 μL of Shengmaiyin (Dangshen Fang) sample, add 80 μL of acetonitrile, centrifuge at high speed for 10 minutes (speed 13000 rpm), take the supernatant, filter it with a 0.45 μm microporous membrane, That is, the test solution was obtained. The chromatographic conditions are: chromatographic column: Agilent Extend C18 column (4.6mm×250mm, 5μm); mobile phase: A is acetonitrile, B is 0.1% formic acid aqueous solution, and the gradient elution is set according to the elution program in the table below; flow rate: 1.0mL / min; column temperature: 25°C; injection volume: 10μL.

[0047] Table 1: Elution program table

[0048]

[0049] Table 1 shows: at the beginning, the volume percentage concentration of mobi...

Embodiment 2

[0055] The selection of embodiment 2 need testing solution preparation conditions

[0056] ①Preparation condition 1 of the test solution: Precisely pipette 920 μL of Shengmaiyin (Dangshen Fang) sample, add 80 μL of acetonitrile, centrifuge at a high speed for 10 minutes (speed 13000 rpm), take the supernatant, and filter it with a 0.45 μm microporous membrane to obtain the Test solution ①, detected by high performance liquid chromatography, chromatographic conditions: chromatographic column: Agilent Extend C18 column (4.6mm×250mm, 5μm); mobile phase: A is acetonitrile, B is 0.1% formic acid aqueous solution, and gradient elution is carried out , the elution schedule is the same as Table 1; flow rate: 1.0mL / min; column temperature: 25°C; detection wavelength: 254nm; injection volume: 10μL. The resulting chromatograms are shown in image 3 .

[0057] ②Preparation condition 2 of the test solution: Accurately measure 10mL of Shengmaiyin (Dangshen Fang) sample, extract 3 times wi...

Embodiment 3

[0062] Example 3 Separation of samples under different gradient elution procedures (taking Shengmaiyin (Dangshen Fang) test solution as an example)

[0063] The preparation method of the test solution is: accurately pipette 920 μL of Shengmaiyin (Dangshen Fang) sample, add 80 μL of acetonitrile, centrifuge at a high speed for 10 minutes (speed 13000 rpm), take the supernatant, and filter it with a 0.45 μm microporous membrane to obtain The test solution.

[0064] 1. Elution procedure 1, see chromatogram Figure 7 .

[0065]

[0066] 2. Elution procedure 2, see chromatogram Figure 8 .

[0067]

[0068] 3. Elution procedure 3, see chromatogram Figure 9 .

[0069]

[0070] 4. Elution procedure 4, see chromatogram Figure 10 .

[0071]

[0072] The above samples were all eluted on an Agilent Extend C18 column (4.6mm×250mm, 5μm), flow rate: 1.0mL / min; column temperature: 25°C; detection wavelength: 254nm; injection volume: 10μL.

[0073] Under the above elutio...

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Abstract

The invention discloses a test method for a pulse-activating preparation adopting pilose asiabell root, which can be used for measuring contents of 5-hydroxymethyl furfural, protocatechuic acid, lobetyolin and schizandrin in the pulse-activating preparation. The method comprises the steps of preparing a sample to be tested into a test product solution, obtaining the high performance liquid chromatogram of the test product solution through high performance liquid chromatographic detection, and then calculating according to standard curvilinear equations prepared through all comparison products to obtain the contents of 5-hydroxymethyl furfural, protocatechuic acid, lobetyolin and schizandrin in the pulse-activating preparation. The test method is high repeatability and stability, and is simple and convenient in operation. The contents of the four compounds are measured through one chromatography condition at the same time, the quality evaluation level of the pulse-activating preparation is improved, and meanwhile a certain basis can be provided for the identification on pilose asiabell root and Chinese magnoliavine fruit in crude medicine.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a content determination method of a compound Chinese medicine preparation, in particular to a content determination method of Shengmai preparation (Dangshen prescription). Background technique [0002] Shengmai (Dangshen Prescription) is a formula based on the traditional ancient prescription Shengmaisan, with Codonopsis ginseng instead of ginseng. Codonopsis Codonopsis in the prescription is the monarch drug, which invigorates the middle and nourishes qi, supplemented by Ophiopogon japonicus to nourish yin and promote body fluid, and schisandra to restrain the lungs and stop sweat. Compared with Yuanmai Formula (Ginseng Formula), Codonopsis Codonopsis is milder. Therefore, Codonopsis Codonopsis is generally used for mild and chronic syndromes, while Ginseng Formula is mostly used for acute and severe diseases. According to the data of the State Food and Drug A...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 颜继忠楚楚徐绍静吴琼琼李行诺
Owner ZHEJIANG UNIV OF TECH
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