Multi-strain microorganism leavening agent and preparation method thereof

A microbial starter and multi-strain technology, which is applied in the field of multi-strain microbial starter and its preparation, can solve the problems of unreachable production and insufficient supply, and achieve the effects of improving quality, increasing production and increasing production

Active Publication Date: 2014-06-04
赣州天绿生化科技有限公司
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still cases where production is in short supply when prices peak
In addition, although the juice yield rate of Gannan

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Bacillus subtilis, Bacillus megaterium, Aspergillus niger, Streptococcus lactis, Candida genus, and Corynebacterium glutamicum were respectively activated and cultured on slant surfaces, wherein the culture medium of Bacillus subtilis was starch 0.15%+ Glucose 0.5% + urea 0.1% + dipotassium hydrogen phosphate 0.3% + potassium dihydrogen phosphate 0.15% + magnesium sulfate 0.05% + yeast extract 0.02% + ferric chloride 0.01% + calcium carbonate 0.01% + soybean meal 1%; Bacillus megaterium The culture medium is 5.0 grams of beef extract, 8.0 grams of peptone, 2.0 grams of glucose, 0.5 grams of magnesium sulfate, 3.0 grams of sodium chloride, 1.0 grams of dipotassium hydrogen phosphate, 1000ml of water, pH = 7.0-7.5, temperature 34 ℃; vegetable milk The culture medium for Bacillus is 5.0 grams of beef extract, 2.5 grams of yeast powder, 10.0 grams of glucose, 0.5 grams of Tween-80, 1.0 grams of ammonium citrate, 2.5 grams of sodium acetate, 0.05 grams of manganese sulfate, 0...

Embodiment 2

[0017] Bacillus subtilis, Bacillus megaterium, Aspergillus niger, Streptococcus lactis, Candida aeruginosa, and Corynebacterium glutamicum are the same as in Example 1 for strain activation and slant culture.

[0018] The ratio of each microbial strain according to the quantity and percentage: Bacillus subtilis 10%, Bacillus megaterium 11%, Lactobacillus plantarum 15%, Streptococcus lactis 17%, Candida genus 28%, Corynebacterium glutamicum 19% , mixed into a conical flask, placed in a shaker, cultured at 28°C for 3 days, controlled at pH 6.5-7.0, so that the number of bacteria reached 600-800 million / ml, and the multi-strain Microbial starter 2.

Embodiment 3

[0020] Bacillus subtilis, Bacillus megaterium, Aspergillus niger, Streptococcus lactis, Candida aeruginosa, and Corynebacterium glutamicum are the same as in Example 1 for strain activation and slant culture.

[0021] The ratio of each microbial strain according to the quantity and percentage: Bacillus subtilis 13%, Bacillus megaterium 16%, Lactobacillus plantarum 11%, Streptococcus lactis 12%, Candida genus 24%, Corynebacterium glutamicum 24% , mixed into a conical flask, placed in a shaker, cultured at 28°C for 2 days, controlling the pH at 6.5-7.0, so that the number of bacteria reached 600-800 million / ml, and the multi-strain Microbial starter 3.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a multi-strain microorganism leavening agent and a preparation method thereof. The preparation method comprises the steps of respectively performing strain activation and slope cultivation on bacillus subtilis, bacillus megatherium, aspergillus niger, lactic streptococci, candida utilis and corynebacterium glutamicum, mixing the six microorganism strains by weight percent, putting the mixed strains into a triangular flask, putting the triangular flask into a shaker, cultivating at 28 DEG C for 2-3 days, controlling the pH to be 6.5-7.0 so that the strain content reaches 0.6-0.8 billions per milliliter, thus obtaining the multi-strain microorganism leavening agent. By use of the multi-strain microorganism leavening agent prepared from the six specific microorganism strains selected according to the characteristics of navel oranges, the quality of the navel oranges can be improved, and the yield of the navel oranges can be increased by 10-15 percent, so that a demand in the highest price stage can be met.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a multi-strain microbial starter and a preparation method thereof. Background technique [0002] Since navel oranges were planted in the 1970s, the Gannan navel orange industry has experienced a difficult and tortuous development path from scratch. Due to the special geographical environment and rich rare earth, Gannan navel oranges are not only delicious, sweet and juicy, but also play a role in health care. However, there are still situations where production is in short supply when prices are at their peak. In addition, although the juice yield rate of Gannan navel oranges is relatively high compared to other domestic navel oranges, it is still not up to the level of Brazil and other countries. For example, the juice yield of Brazilian industrial oranges can reach more than 60%, while the juice yield of Gannan navel oranges can only reach about 40% at most, and the solids l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C12R1/125C12R1/11C12R1/15C12R1/46C12R1/685C12R1/72
Inventor 肖梅
Owner 赣州天绿生化科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap