A kind of active ingredient detection method of succulent tea plant
A technology of textured fragrant tea vegetables and a detection method, which is applied in the field of detection of active ingredients of fiber-flowered thread-patterned fragrant tea vegetables, can solve problems that have not been discovered and are not recorded, achieve good specificity and quality correlation, and have small error in results , the effect of high content
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Embodiment 1
[0066] Determination of the content of iso-schaftoside, schaftoside and rosmarinic acid in Xianhuaxianwen fragrant tea by high performance liquid chromatography:
[0067] 1) Prepare the reference solution:
[0068] Accurately weigh 10 mg of isoschaftoside reference substance, put it in a 100ml volumetric flask, add a small amount of 70% (volume) methanol aqueous solution to dissolve, set the volume to the mark, shake well, and prepare isoxafotoside with a concentration of 0.15 mg / ml Tacoside reference substance solution, and stored in a refrigerator at 4°C for later use.
[0069] The same method was used to prepare the reference substance solution of schiaftoside and rosmarinic acid.
[0070] 2) Prepare the test solution:
[0071] Take 1g of Xianhuaxianwen Fragrant Tea Vegetables medicinal material, accurately weigh it, put it in a 100ml conical flask, add 25ml of 70% (volume) methanol aqueous solution precisely, weigh it, extract it by ultrasonic for 30min, after cooling, a...
Embodiment 2
[0083] The methodological investigation of the detection method of the active ingredient of the fiber flower thread pattern fragrant tea dish of the present invention:
[0084] (1) Linear relationship
[0085] Using the same operating steps and conditions as in Example 1, accurately measure the appropriate amount of iso-schafutaside, schafutaside and rosmarinic acid reference solution respectively, inject a sample for analysis, and use the injection volume to the integral value of the chromatographic peak area Perform linear regression processing to obtain the regression line equations, correlation coefficients and linear ranges of isoschaftosides, schafutasides and rosmarinic acid, as shown in Table 1. The results show that the three are within the corresponding ranges and have good linearity relationship, see Figure 7 , Figure 8 , Figure 9 .
[0086] Table 1: Linear inspection experimental results
[0087]
[0088] (2) Precision experiment
[0089] Using the same...
Embodiment 3
[0105] Selection and optimization of gradient elution conditions:
[0106] (1) Gradient elution conditions: 0~30min, mobile phase A is 25%, mobile phase B is 75%;
[0107] 30~70min, the mobile phase A is 25%→40%, and the mobile phase B is 75%→60%. See image 3 .
[0108] (2) Gradient elution conditions: 0~70min, 40% mobile phase A, 60% mobile phase B. See Figure 4 .
[0109] from image 3 with Figure 4 It can be seen that the above gradient elution conditions (1) and (2) cannot separate the target peaks well
[0110] (3) Gradient elution conditions: 0~30min, mobile phase A is 30%, mobile phase B is 70%;
[0111] 30~70min, the mobile phase A is 30%→50%, and the mobile phase B is 70%→50%. See Figure 5 .
[0112] from Figure 5 It can be seen that the target peak can be separated better, so this elution condition was chosen.
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