Novel dual-layer composite bone tissue engineering scaffold and preparation method thereof
A bone tissue engineering, double-layer composite technology, applied in the field of biomedicine, can solve problems such as life-threatening, immune rejection, foreign body rejection, etc., to improve cell affinity and biological activity, and avoid aseptic inflammation. Response, the effect of reducing aseptic inflammatory response
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[0066] The following examples are used to illustrate the present invention. It should be understood that the examples are used to illustrate the present invention and not to limit the present invention. The scope and core content of the present invention are determined based on the claims.
[0067] 1. Preparation of outer bacterial cellulose scaffold by vacuum freeze-drying method
[0068] (1) Main reagents:
[0069] Bacterial cellulose was purchased from Hainan Coconut Fruit Food Co., Ltd.; NaOH was analytically pure and purchased from Chengdu Kelon Chemical Reagent Factory.
[0070] (2) Instruments and equipment
[0071] Vacuum freeze dryer (Model FD-1, Beijing Boyikang Experimental Instrument Co., Ltd., Beijing, China).
[0072] (3) Experimental method
[0073] ① Cleaning: Put the bacterial cellulose into 0.2-0.3mol / L NaOH lye, boil for 20-40 minutes, rinse with distilled water 4-5 times until the pH value is neutral, and obtain high-purity bacterial cellulose Thin bracket.
[0074] ②...
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