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Magnetic field assisted dielectrophoresis enrichment method for nanoparticle labeled immunodetection

A nanoparticle and immunoassay technology, applied in the field of biomedicine, can solve unreported problems and achieve low cost, high separation and enrichment efficiency, high sensitivity and specificity

Active Publication Date: 2014-06-18
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports at home and abroad on the isolation and enrichment of the immune reaction aggregates labeled with nanoparticles (antibodies) from the mixture in situ

Method used

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  • Magnetic field assisted dielectrophoresis enrichment method for nanoparticle labeled immunodetection
  • Magnetic field assisted dielectrophoresis enrichment method for nanoparticle labeled immunodetection

Examples

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Embodiment 1

[0030]Enrichment of rare earth fluorescent sensing nanoparticles and iron oxide nanoparticles-labeled sandwich method coupled aggregates for detection of hepatitis B antigen:

[0031] The test object is HBsAg-positive human serum; the sensing nanoparticles are erbium and ytterbium doped sodium fluoroyttrium upconversion nanoparticles, and the surface is modified with carboxyl groups. The size is about 20 nanometers, and the deviation is less than 2 nanometers. , see references for the preparation method A.D.Ostrowski, E.M.Chan, D.J.Gargas, et al.Controlled synthesis and single-particle imaging of bright, sub-10nm lanthanide-doped upconverting nanoparticles, ACS NANO, 2012, 6, 2686-2692 and H.P.Zhou ,C.H.Xu,W.Sun,C.H.Yan,Clean and flexible modification strategy for carboxyl / aldehyde-functionalized upconversion nanoparticles and their optical applications,Adv.Func.Mater.,2009,19,3892-3900, iron oxide nanoparticles as surface Carboxyl-modified ferric oxide, with an average size o...

Embodiment 2

[0034] Detection of human herpes virus (EB virus) VCA-IgA antibody gold nanoparticles, iron oxide nanoparticles indirect coupling aggregate enrichment: the test substance is EB virus VCA-IgA antibody-positive human serum; sensing nanoparticles adopt Gold nanoparticles with a size of 20 nanometers, with a deviation of less than 2 nanometers, and with carboxyl groups on the surface, as prepared by reference B.C.Mei, K.Susumu, I.L.Medintz, J.B.Delehanty, T.J.Mountziaris, H.Mattoussi, Modular poly(ethylene glycol) ligands for biocompatible semiconductor and gold nanocrystals with extended pH and ionic stability, J.Mater.Chem.2008, 18, 45949-4958., iron oxide nanoparticles are surface carboxyl-modified ferric oxide, with an average size of 8 nanometers and a deviation of 2 Nano. Mix the gold nanoparticles with the Epstein-Barr virus NA1 recombinant antigen solution (PBS, the concentration is 0.01 mg / ml) at a concentration of 0.1 mg / ml, and add 1-ethyl-(3-dimethylaminopropyl)carbodi...

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Abstract

The invention discloses a magnetic field assisted dielectrophoresis enrichment method for nanoparticle labeled immunodetection. By utilizing a nanoparticle labeled antibody or antigen, a method for screening and enriching aggregate formed by performing immunoconjugate on a magnetic nanoparticle and sensing nanoparticle labeled antibody or antigen by using magnetic field assisted dielectrophoresis is designed. An alternating electric field is applied between parallel non-uniform electrodes, spatial directional movement of nanoscale particles in the solution is realized, selective enrichment of nanoparticle and magnetic nanoparticle immunoreaction aggregate in a micro area on the surface of a transparent electrode in a complex solution is realized in a specific voltage magnitude and frequency and magnetic field intensity range, and the method is used for sensitivity immunodetection.

Description

technical field [0001] The present invention relates to a biological detection method in the field of biomedicine, specifically a magnetic field-assisted dielectrophoresis technology, which realizes the selective detection of sensing nanoparticles and magnetic nanoparticle aggregates of labeled antibodies (or antigens) that undergo immune reactions. Methods for Microdomain Enrichment. Background technique [0002] Immunoassays are realized by signal-sensing nanoparticle labeling, which has the advantages of high sensitivity, high environmental adaptability, and quantification. At present, different nanoparticles are used to detect effects such as fluorescence, surface plasmon luminescence, and surface-enhanced Raman scattering. A variety of biological detection devices and reagents, such as: labeling antibodies with quantum dots, measuring the content of antigens in serum with the sandwich method, and making various surface-immobilized or liquid-phase detection kits; bonding...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/561
CPCG01N33/54326G01N33/54346G01N33/561G01N33/577G01N2550/00
Inventor 王萌王洁巴龙
Owner SOUTHEAST UNIV
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