Preparation method of probiotic-containing compound plant enzyme

A plant enzyme and probiotic technology, which is applied in the field of compound plant enzyme preparation, can solve the problems of large differences in fermentation metabolites, low active ingredient content, single enzyme component, etc., to achieve good stability, fully coordinated fermentation, and complete extraction Effect

Inactive Publication Date: 2014-06-25
田雷
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AI-Extracted Technical Summary

Problems solved by technology

Therefore, a single enzyme has a single component and can only catalyze a certain biological macromolecule, which is not suitable for a variety of biological macromolecular substrates in the biological world. Therefore, people have carried out in-depth research on compound enzymes, and their development is becoming more and more important. focus on
[0004] However, most of the existing common compound enzymes are fe...
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Abstract

The invention discloses a preparation method of a probiotic-containing compound plant enzyme. The probiotic-containing compound plant enzyme is prepared by selecting various vegetables and medicinal and edible plants as fermentation substrates and adopting various probiotics/yeasts as fermentation inoculums through multi-period discontinuous fed-batch fermentation and multi-stage deep fermentation. The probiotic-containing compound plant enzyme prepared through the preparation method has the advantages of various types of contained enzymes, high activity, good stability and small influence from environment. By adopting the method, different substrates can be fermented by using different bacteria in different time periods, so that different active ingredients contained in various fermentation substrates can be more completely extracted and the fermentation of the whole system can be more sufficient and harmonious.

Application Domain

Food ingredient functionsFood preparation

Technology Topic

HorticultureSubmerged fermentation +11

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  • Preparation method of probiotic-containing compound plant enzyme

Examples

  • Experimental program(2)
  • Effect test(2)

Example Embodiment

[0034] Example 1
[0035] Step 1: Primary fermentation: Take 5 equal parts (mass) of black rice, rice, millet, mung bean and black bean as the fermentation substrate for primary fermentation. After ozone disinfection treatment, mix evenly under clean conditions, and the inoculum amount is 5wt The Dirk yeast fermentation broth was added as a fermentation inoculum at a ratio of %, and the fermentation temperature was controlled at 40°C-45°C for 20-40 days, and the first-level fermentation was performed to obtain the first-level fermentation product.
[0036] Step 2: Secondary fermentation: Take 5 equal parts (quality) of fungus, white fungus, onion, broccoli and celery after ozone sterilization and mix them evenly, as secondary fermentation vegetables and fungi fermentation substrate, as in the first level in step 1. The primary fermentation product obtained after fermentation is mixed according to the mass ratio of 1:1 as the secondary fermentation substrate, and the P. pastoris fermentation broth is added as the fermentation inoculum in the proportion of 5 wt% of the inoculum, and the fermentation temperature is 5-10 days. Control at 32°C-37°C and perform secondary fermentation to obtain secondary fermentation product.
[0037] Step 3: Tertiary fermentation: Take 5 equal parts (quality) of wolfberry, honeysuckle, pueraria lobata, mulberry leaf and osmanthus osmanthus and mix them evenly after ozone disinfection, as a dual-purpose plant fermentation substrate for medicine and food for tertiary fermentation, as in step 2 The second-stage fermentation product obtained after the first-stage fermentation is mixed according to the mass ratio of 1:2 as the third-stage fermentation substrate, and the fermentation broth of Streptococcus thermophilus is added as the fermentation inoculum in the proportion of 7wt% of the inoculum, and the fermentation is carried out for 5-10 days. The fermentation temperature is controlled at 32℃-37℃, and the tertiary fermentation is carried out to obtain the tertiary fermentation product.
[0038] Step 4: Four-stage fermentation: Take 5 equal parts (quality) of apples, pears, hawthorns, lemons and oranges and mix them evenly after ozone sterilization. They are used as the fruit fermentation substrate of the fourth-stage fermentation, and the third-stage fermentation obtained in step 3 The grade fermented product is mixed according to the mass ratio of 1:3 as the fourth grade fermentation substrate, and the fermentation broth of Bacillus amyloliquefaciens is added as the fermentation inoculum at the ratio of 5wt% of the inoculum, and the fermentation temperature is controlled at 28°C for 5-10 days. , Carry out four-stage fermentation to obtain the final compound plant enzyme.
[0039] After testing, the final compound plant enzyme contains 7 enzymes, among which amylase activity is 265U/g, lipase activity is 78U/g, protease activity is 14U/g, cellulase activity is 22U/g, β-glucan Enzyme activity was 15U/g, xylanase activity was 5U/g, and lactase activity was 8U/g.
[0040] The compound plant enzyme containing probiotics prepared in this embodiment can quickly degrade the molecular structure of organic substances into simpler structures, and be digested by microorganisms into final inert substances to achieve the decomposition of various compounds, organic substances, and toxins. It is used in products such as hangover, liver protection, whitening, and repair of skin damage.

Example Embodiment

[0041] Example 2
[0042] Step 1: Primary fermentation: Take 3 equal parts (mass) of black rice, rice and mung bean as the fermentation substrate for primary fermentation, and after ozone disinfection treatment, mix well under clean conditions and add in proportion of 7wt% of inoculation amount The Dirk yeast fermentation broth was used as the fermentation inoculum, and the fermentation temperature was controlled at 40°C for 20 days, and the primary fermentation was performed to obtain the primary fermentation product.
[0043] Step 2: Secondary fermentation: Take 2 equal parts (mass) of fungus and white fungus and mix them evenly after ozone disinfection, as the usable fungus fermentation substrate for secondary fermentation, and the primary fermentation product obtained after primary fermentation in step 1. The mass ratio of 2:3 was mixed as the secondary fermentation substrate, and the P. pastoris fermentation broth was added as the fermentation inoculum at a ratio of 5 wt% of the inoculum. The fermentation was carried out for 2 days, and the fermentation temperature was controlled at 35°C. Grade fermented product.
[0044] Step 3: Tertiary fermentation: Take 2 equal parts (quality) of Osmanthus fragrans and rose after ozone sterilization and mix them evenly, as a dual-purpose plant fermentation substrate for tertiary fermentation, and the secondary fermentation obtained after secondary fermentation in step 2. The substances were mixed according to the mass ratio of 2:5 as the three-stage fermentation substrate, and the fermentation broth of Streptococcus thermophilus was added as the fermentation inoculum in the proportion of 7wt% of the inoculum. The fermentation was carried out for 5 days, and the fermentation temperature was controlled at 32°C. Fermentation to obtain a tertiary fermentation product.
[0045] Step 4: Four-stage fermentation: Take 3 equal parts (mass) of lemon, strawberry and sweet orange and mix them evenly after ozone sterilization, as the fruit fermentation substrate for four-stage fermentation, and the third-stage fermentation product obtained after the third-stage fermentation in step 3. Mix according to the mass ratio of 3:7 as the fourth-stage fermentation substrate, and add the fermentation broth of Bacillus amyloliquefaciens as the fermentation inoculum in the proportion of 5wt% of the inoculum, and ferment for 2 days. The fermentation temperature is controlled at 28℃, and the fourth-stage fermentation is carried out. Get the final compound plant enzyme.
[0046] After testing, the final composite plant enzyme contains 7 enzymes, among which amylase activity is 282U/g, lipase activity is 55U/g, protease activity is 8U/g, cellulase activity is 10U/g, β-glucan Enzyme activity was 15U/g, xylanase activity was 5U/g, and lactase activity was 6U/g.
[0047] The compound plant enzyme containing probiotics prepared in this embodiment can quickly degrade the molecular structure of organic substances into simpler structures, and then digest them into the final inert substances by microorganisms, and realize the decomposition of various compounds, organic substances, and toxins. Used in products such as whitening and repairing skin damage.
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