Preparation method of probiotic-containing compound plant enzyme

A plant enzyme and probiotic technology, which is applied in the field of compound plant enzyme preparation, can solve the problems of large differences in fermentation metabolites, low active ingredient content, single enzyme component, etc., to achieve good stability, fully coordinated fermentation, and complete extraction Effect

Inactive Publication Date: 2014-06-25
田雷
View PDF3 Cites 60 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, a single enzyme has a single component and can only catalyze a certain biological macromolecule, which is not suitable for a variety of biological macromolecular substrates in the biological world. Therefore, people have carried out in-depth research on compound enzymes, and their development is becoming more and more important. focus on
[0004] However, most of the existing common compound enzymes are fe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of probiotic-containing compound plant enzyme

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1

[0035] Step 1: Primary fermentation: Take 5 equal parts (mass) of black rice, rice, millet, mung bean and black bean as the fermentation substrate for primary fermentation. After ozone disinfection treatment, mix evenly under clean conditions, and the inoculum amount is 5wt The Dirk yeast fermentation broth was added as a fermentation inoculum at a ratio of %, and the fermentation temperature was controlled at 40°C-45°C for 20-40 days, and the first-level fermentation was performed to obtain the first-level fermentation product.

[0036] Step 2: Secondary fermentation: Take 5 equal parts (quality) of fungus, white fungus, onion, broccoli and celery after ozone sterilization and mix them evenly, as secondary fermentation vegetables and fungi fermentation substrate, as in the first level in step 1. The primary fermentation product obtained after fermentation is mixed according to the mass ratio of 1:1 as the secondary fermentation substrate, and the P. pastoris fer...

Example Embodiment

[0041] Example 2

[0042] Step 1: Primary fermentation: Take 3 equal parts (mass) of black rice, rice and mung bean as the fermentation substrate for primary fermentation, and after ozone disinfection treatment, mix well under clean conditions and add in proportion of 7wt% of inoculation amount The Dirk yeast fermentation broth was used as the fermentation inoculum, and the fermentation temperature was controlled at 40°C for 20 days, and the primary fermentation was performed to obtain the primary fermentation product.

[0043] Step 2: Secondary fermentation: Take 2 equal parts (mass) of fungus and white fungus and mix them evenly after ozone disinfection, as the usable fungus fermentation substrate for secondary fermentation, and the primary fermentation product obtained after primary fermentation in step 1. The mass ratio of 2:3 was mixed as the secondary fermentation substrate, and the P. pastoris fermentation broth was added as the fermentation inoculum at a ratio of 5 wt% of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of a probiotic-containing compound plant enzyme. The probiotic-containing compound plant enzyme is prepared by selecting various vegetables and medicinal and edible plants as fermentation substrates and adopting various probiotics/yeasts as fermentation inoculums through multi-period discontinuous fed-batch fermentation and multi-stage deep fermentation. The probiotic-containing compound plant enzyme prepared through the preparation method has the advantages of various types of contained enzymes, high activity, good stability and small influence from environment. By adopting the method, different substrates can be fermented by using different bacteria in different time periods, so that different active ingredients contained in various fermentation substrates can be more completely extracted and the fermentation of the whole system can be more sufficient and harmonious.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a preparation method of a compound plant enzyme containing probiotics. Background technique [0002] After more than 50 years of development, China's enzyme preparation industry has entered the ranks of the world's largest enzyme preparation countries. At present, there are about 30 kinds of enzyme preparations that have achieved large-scale production, but most of them are single enzyme preparations, mainly glucoamylase, amylase, phytase, cellulase, protease, pectinase, etc., of which glucoamylase, starch The output of enzymes and phytases exceeds 10,000 tons, and the main application fields are concentrated in the starch sugar industry, beer industry, dairy industry, baking industry, fuel alcohol industry, wine and beverage industry, detergent industry, paper industry, etc., in food applications It is still in the stage of use as a food additive. Its effective...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23L1/29A23L33/00
CPCA23L33/135A23V2002/00A23V2200/30
Inventor 田雷
Owner 田雷
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap