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Method for cultivating melia azedarach saplings by adopting tissue culture technology

A technique, a technique of tissue culture, which is applied in the field of cultivating neem seedlings by tissue culture technology, can solve the problems of small breeding quantity, intolerance to transplanting, labor-intensive, etc., and achieve the effects of broad development prospects, beautifying the environment, and improving the quality of life

Inactive Publication Date: 2014-07-02
天津市金三农农业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two breeding methods for neem tree, seed propagation and root propagation, but the emergence rate is low, the seedling growth is weak, the root system is few, and it is not resistant to transplanting and other factors.
The current breeding mode of neem tree has a very small number of breeding per year, which is time-consuming and labor-intensive, which invisibly increases the capital investment rate. It is difficult to meet the current market demand with half the effort.

Method used

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  • Method for cultivating melia azedarach saplings by adopting tissue culture technology
  • Method for cultivating melia azedarach saplings by adopting tissue culture technology
  • Method for cultivating melia azedarach saplings by adopting tissue culture technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Macroelement (Mother Liquor Ⅰ) mg / L

[0065] NH 4 NO 3 34000

[0066] KNO 3 40000

[0067] CaCl 2 2H2O 8500

[0068] MgSO 4 7H2O 6800

[0069] K H 2 PO 4 3300

[0070] Trace elements (mother liquor Ⅱ)

[0071] KI 180

[0072] h 3 BO 3 1140

[0073] MnSO 4 4H 2 O 4250

[0074] ZnSO 4 ·7H 2 O 1720

[0075] Na 2 MoO 4 2H 2 O 60

[0076] CuSO4·5H 2 O 4.5

[0077] CoCl 2 ·6H 2 O 4.5

[0078] Iron salt (mother liquor Ⅲ)

[0079] FeSO 4 ·7H 2 O 5560

[0080] Na 2 -EDTA·2H 2 O 7400

[0081] Organic ingredients (mother liquor Ⅳ)

[0082] IVA inositol 19800

[0083] IVB Niacin 100

[0084] Pyridoxine Hydrochloride (Vitamin B 6 ) 100

[0085] Thiamine Hydrochloride (Vitamin B 1 ) 100

[0086] Glycine 400

[0087] The consumption of above-mentioned various nutrient components, except that mother liquor I is 20 times of concentrated solution, all the other are 200 times of concentrated solution. Also need to add to the culture medium:...

Embodiment 2

[0094] The proportioning of culture solution of rooting medium is:

[0095] Macroelement (Mother Liquor Ⅰ) mg / L

[0096] NH 4 NO 3 (ammonium nitrate) 34000

[0097] KNO 3 (potassium nitrate) 40000

[0098] CaCl 2 2H 2 O (calcium chloride dihydrate) 8500

[0099] MgSO 4 ·7H 2 O (magnesium sulfate heptahydrate) 6800

[0100] K H 2 PO 4 (potassium dihydrogen phosphate) 3300

[0101] Trace elements (mother liquor Ⅱ) mg / L

[0102] KI (potassium iodide) 180

[0103] h 3 BO 3 (boric acid) 1140

[0104] MnSO 4 4H 2 O (manganese sulfate tetrahydrate) 4250

[0105] ZnSO 4 ·7H 2 O (zinc sulfate heptahydrate) 1720

[0106] Na 2 MoO 4 2H 2 O (sodium molybdate dihydrate) 60

[0107] CuSO 4 ·5H 2 O (copper sulfate pentahydrate) 4.5

[0108] CoCl 2 ·6H 2 O (cobalt chloride hexahydrate) 4.5

[0109] Iron salt (mother liquor Ⅲ) mg / L

[0110] FeSO 4 ·7H 2 O (iron sulfide heptahydrate) 5560

[0111] Na 2 -EDTA·2H 2 O (dihydrate EDTA sodium salt) 7400

[0...

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Abstract

The invention discloses a method for cultivating melia azedarach saplings by adopting a tissue culture technology. The method comprises the steps of disinfecting a plant material, inoculating the plant material to an induction medium to induce a callus, inoculating the callus to a differential medium to differentiate seedlings, inoculating the seedlings to a rooting medium, and transplanting the complete plant to a field. The melia azedarach saplings cultivated by the tissue culture technology are planted, then bear fruit after 3-4 years and enter the rich phase after 5-8 years; the yield per unit area is about 1 ton; the production period is about 200 years; the melia azedarach saplings are one of first choice tree seeds for city greening.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation and relates to a method for cultivating neem saplings, more specifically a method for cultivating neem saplings by adopting tissue culture technology. Background technique [0002] Neem, also known as Neem, is a deciduous tree with a broad and flat crown. Alternate leaves, 2-3 odd-pinnate compound leaves; leaflets opposite, ovate or lanceolate, serrate thick and blunt; old leaves glabrous. Flowers bisexual, light blue, fragrant, in axillary panicles. It blooms in April-May and matures in October-November. The drupe is oval or round. The neem tree grows fast and likes sunlight. Neem tree planting starts in 3-4 years and bears fruit in 5-8 years. It enters the rich production period, with a seed yield of about 1 ton per mu; the production period can last for about 200 years, and it is one of the preferred tree species for urban greening. Neem bonsai is elegant and generous, which can be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱博
Owner 天津市金三农农业科技开发有限公司
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