Method for cultivating melia azedarach saplings by adopting tissue culture technology
A technique, a technique of tissue culture, which is applied in the field of cultivating neem seedlings by tissue culture technology, can solve the problems of small breeding quantity, intolerance to transplanting, labor-intensive, etc., and achieve the effects of broad development prospects, beautifying the environment, and improving the quality of life
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Embodiment 1
[0064] Macroelement (Mother Liquor Ⅰ) mg / L
[0065] NH 4 NO 3 34000
[0066] KNO 3 40000
[0067] CaCl 2 2H2O 8500
[0068] MgSO 4 7H2O 6800
[0069] K H 2 PO 4 3300
[0070] Trace elements (mother liquor Ⅱ)
[0071] KI 180
[0072] h 3 BO 3 1140
[0073] MnSO 4 4H 2 O 4250
[0074] ZnSO 4 ·7H 2 O 1720
[0075] Na 2 MoO 4 2H 2 O 60
[0076] CuSO4·5H 2 O 4.5
[0077] CoCl 2 ·6H 2 O 4.5
[0078] Iron salt (mother liquor Ⅲ)
[0079] FeSO 4 ·7H 2 O 5560
[0080] Na 2 -EDTA·2H 2 O 7400
[0081] Organic ingredients (mother liquor Ⅳ)
[0082] IVA inositol 19800
[0083] IVB Niacin 100
[0084] Pyridoxine Hydrochloride (Vitamin B 6 ) 100
[0085] Thiamine Hydrochloride (Vitamin B 1 ) 100
[0086] Glycine 400
[0087] The consumption of above-mentioned various nutrient components, except that mother liquor I is 20 times of concentrated solution, all the other are 200 times of concentrated solution. Also need to add to the culture medium:...
Embodiment 2
[0094] The proportioning of culture solution of rooting medium is:
[0095] Macroelement (Mother Liquor Ⅰ) mg / L
[0096] NH 4 NO 3 (ammonium nitrate) 34000
[0097] KNO 3 (potassium nitrate) 40000
[0098] CaCl 2 2H 2 O (calcium chloride dihydrate) 8500
[0099] MgSO 4 ·7H 2 O (magnesium sulfate heptahydrate) 6800
[0100] K H 2 PO 4 (potassium dihydrogen phosphate) 3300
[0101] Trace elements (mother liquor Ⅱ) mg / L
[0102] KI (potassium iodide) 180
[0103] h 3 BO 3 (boric acid) 1140
[0104] MnSO 4 4H 2 O (manganese sulfate tetrahydrate) 4250
[0105] ZnSO 4 ·7H 2 O (zinc sulfate heptahydrate) 1720
[0106] Na 2 MoO 4 2H 2 O (sodium molybdate dihydrate) 60
[0107] CuSO 4 ·5H 2 O (copper sulfate pentahydrate) 4.5
[0108] CoCl 2 ·6H 2 O (cobalt chloride hexahydrate) 4.5
[0109] Iron salt (mother liquor Ⅲ) mg / L
[0110] FeSO 4 ·7H 2 O (iron sulfide heptahydrate) 5560
[0111] Na 2 -EDTA·2H 2 O (dihydrate EDTA sodium salt) 7400
[0...
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