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Method for separating and purifying curdlan by use of flocculant

A technology of separation, purification and flocculant, applied in the field of industrialized production of curdlan gum, which can solve the problems of low gel strength, high ion concentration, low purity, etc., and achieve the effects of stable performance, energy saving and solvent consumption reduction

Active Publication Date: 2014-07-02
山东萃源亿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned prior art, in order to solve the problems of low purity, high ion concentration, low gel strength and low gel transparency of curdlan products at present, the present invention provides a kind of curdlan purified by flocculant glue method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0022] Experimental Example 1 Selection of flocculant

[0023] The flocculant polyacrylamide, polyaluminum chloride (PAS), bentonite (sodium-based bentonite with 80% montmorillonite content), and chitosan for flocculation and clarification were selected respectively for the following experiments:

[0024] Get curdlan gum fermentation liquid, join in the sodium hydroxide solution, constantly stir it and make it dissolve completely to obtain curdlan gum solution, control the concentration of curdlan gum in the solution solution to be 1.0% (w / v, g / ml), [OH -] The concentration is 0.50mol / L; then the solution is divided into four parts, and the flocculant polyacrylamide 0.5‰ (w / v, g / ml) and polyaluminum chloride 0.5% (w / v , g / ml), bentonite 0.5% (w / v, g / ml), chitosan 0.5% (w / v, g / ml), stirred and mixed, and left to settle to remove impurities. The settling time is 8h, and the control The temperature is 25°C; observe the effect of flocculation and sedimentation.

[0025] Result...

Embodiment 1

[0027] Embodiment 1 Utilizes flocculant to separate and purify curdlan

[0028] Proceed as follows:

[0029] (1) Dilute alkali dissolution: take curdlan fermentation liquid, add it to sodium hydroxide solution, stir continuously to dissolve it completely, and obtain a solution, control the concentration of curdlan gum in the solution to 1.5% (w / v, g / ml), [OH - ] concentration is 0.75mol / L;

[0030] (2) Flocculant sedimentation and impurity removal: add sodium-based bentonite to the solution, the concentration of bentonite is 0.6% (w / v, g / ml), stir and mix well, then let it settle and remove impurities, the sedimentation time is 5h, control The temperature is at 25°C;

[0031] (3) Neutralization and precipitation: After the above precipitation is completed, solid-liquid separation is carried out to obtain a clear curdlan solution, which is neutralized by adding dilute hydrochloric acid (the mass concentration of HCl in dilute hydrochloric acid is 10%) until the pH reaches 7...

Embodiment 2

[0034] Embodiment 2 utilizes flocculant to separate and purify curdlan gum

[0035] Proceed as follows:

[0036] (1) Dilute alkali solution: Add the crude product of curdlan gum to the sodium hydroxide solution, stir continuously to dissolve it completely to obtain a solution, and control the concentration of curdlan gum in the solution to 1.0% (w / v , g / ml), [OH - ] concentration is 0.50mol / L;

[0037] (2) Flocculant sedimentation and impurity removal: Add sodium-based bentonite to the solution, the concentration of bentonite is 0.3% (w / v, g / ml), stir and mix well, then let it settle and remove impurities, the sedimentation time is 5h, control The temperature is at 28°C;

[0038] (3) Neutralization and precipitation: After the above precipitation is completed, solid-liquid separation is carried out to obtain a clear curdlan solution, which is neutralized by adding dilute hydrochloric acid (the mass concentration of HCl in dilute hydrochloric acid is 10%) until the pH reache...

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Abstract

The invention discloses a method for separating and purifying curdlan by use of a flocculant. The method comprises the steps of dissolving curdlan fermentation broth or a crude curdlan product in an alkaline solution, adding the flocculant to settle microbial bacteria and insoluble impurities, adding acid in supernatant after separation to neutralize to form curdlan neutralization gel, filtering and concentrating by using an ultrafiltration membrane to obtain the curdlan neutralization gel, adding water to wash and desalt, finally dewatering and drying through an organic solvent to obtain a high-quality curdlan product. The dry purity of the product exceeds 95%, the gel strength is increased to 950-1200 g / cm<2>, and the performance is stable. The flocculant is selected from one or several of polyacrylamide, bentonite and chitosan, and is preferably sodium bentonite. Compared with the existing extraction technology, the extraction and purification process disclosed by the invention has the advantages that the product performance is improved, the solvent consumption is decreased by 80%, and the energy consumption is greatly saved.

Description

technical field [0001] The invention relates to a method for refining curdlan gum, in particular to a method for purifying curdlan gum by using a flocculation process, and belongs to the technical field of curdlan gum industrial production. Background technique [0002] Curdlan gum (Curdlan) is a microbial exopolysaccharide, which has the unique property of forming a gel under heating conditions, also known as thermogel or thermocoagulation polysaccharide. Curdlan gum molecules are unbranched homogeneous polysaccharide polymers connected by β-1,3-D-glucosidic bonds, composed of about 300 to 500 glucose residues, with an average relative molecular weight of about 74000D (Daltons) . Curdlan gum is safe and non-toxic to the human body, insoluble in water but soluble in alkaline solution to form a viscous solution. Curdlan gum is widely used in the food industry as a food additive, which can improve the water holding capacity, viscoelasticity and stability of the product, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 吉武科董学前张永刚武琳刘建军
Owner 山东萃源亿康生物科技有限公司
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