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Preparation method and preparation agent of nano lipidosome compound IgY resisting Helicobacter pylori, Pylori bacteria as well as related enzymes and adhesin of Helicobacter pylori and Pylori bacteria

A technology against Helicobacter pylori and Helicobacter pylori, applied in the direction of liposome delivery, antibody, drug combination, etc., can solve the impossible to achieve effective treatment, prevention, destruction and other problems, and achieve the effect of preventing Helicobacter pylori infection

Pending Publication Date: 2014-08-06
SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The latest research further reveals that Helicobacter pylori urease, phospholipase and Helicobacter pylori adhesin also play an important role in the pathogenic mechanism of Helicobacter pylori. It is the material basis for the adhesion and colonization of bacteria on the gastric and duodenal mucosal surface to cause inflammation, ulcers and even gastric cancer, and directly participates in the destruction of the gastric and duodenal mucosal barrier; urease and phospholipase and adhesin, it is impossible to achieve the purpose of effective treatment and prevention

Method used

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  • Preparation method and preparation agent of nano lipidosome compound IgY resisting Helicobacter pylori, Pylori bacteria as well as related enzymes and adhesin of Helicobacter pylori and Pylori bacteria
  • Preparation method and preparation agent of nano lipidosome compound IgY resisting Helicobacter pylori, Pylori bacteria as well as related enzymes and adhesin of Helicobacter pylori and Pylori bacteria
  • Preparation method and preparation agent of nano lipidosome compound IgY resisting Helicobacter pylori, Pylori bacteria as well as related enzymes and adhesin of Helicobacter pylori and Pylori bacteria

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preparation example Construction

[0033] The preparation method of nanoliposome anti-helicobacter pylori and pylori and its related enzyme and adhesin complex IgY of the present invention comprises the following steps:

[0034] S1. Prepare Helicobacter pylori and pylori composite antigens, and Helicobacter pylori urease subunit B (UreB) and phospholipase A respectively 2 (PLA 2 ), adhesin HpaA, adhesin SabA and adhesin BabA2 gene recombinant expression protein complex antigen.

[0035] Among them, the composite antigen of Helicobacter pylori and pylori is drug-resistant or standard Helicobacter pylori and pylori composite antigen, and the standard refers to non-drug resistance. The compound antigen preparation comprises the following steps:

[0036] S1.1. Obtain Helicobacter pylori and pylori respectively by culturing corresponding bacterial strains. The bacterial strain of Helicobacter pylori can be the drug-resistant Helicobacter pylori strain that is resistant to amoxicillin and clarithromycin or other a...

Embodiment 1

[0116] Production of nano-liposomal anti-drug-resistant Helicobacter pylori and pylori and its related enzymes and adhesins complex IgY hard capsules. The formula is shown in the table below:

[0117] Table 4. Recipe

[0118]

[0119] Process:

[0120] 1. Freeze-dry the basic solution of nano-liposomized anti-drug-resistant Helicobacter pylori and pylori and its related enzymes and adhesin complex IgY to make nano-liposomized anti-drug-resistant Helicobacter pylori and pylori and other Relevant enzymes and adhesin complex IgY dry powder.

[0121] 2. Mix nano-liposomized anti-drug-resistant Helicobacter pylori and pylori and its related enzymes and adhesin compound IgY dry powder with medicinal glucose in a weight ratio of 1:1 according to the formula, and stir well.

[0122] 3. Quantitatively divide the compound IgY dry powder and medicinal glucose mixture into medicinal capsules with a capsule filling machine, each containing 300 mg.

[0123] 4. The capsules are bottle...

Embodiment 2

[0125] Production of nano-liposomal anti-drug-resistant Helicobacter pylori and pylori and its related enzymes and adhesin complex IgY tablets. The formula is shown in the table below:

[0126] Table 5. Recipe

[0127]

[0128] Process:

[0129] 1. Sorbitol is passed through a 60-mesh sieve twice for later use.

[0130] 2. Disperse carboxymethyl cellulose in 30% ethanol to make 1% ethanol solution.

[0131] 3. Use an appropriate amount of item 1 to make soft materials, granulate with a 14-mesh sieve, ventilate and dry at 60°C, and granulate with a 18-mesh sieve. Use a 40-mesh sieve to sieve an appropriate amount of fine powder and mix well with nanoliposome anti-drug-resistant Helicobacter pylori and pylori and its related enzymes and adhesin complex IgY dry powder.

[0132] 4. Stir in magnesium stearate, mix evenly with the whole batch of granules, and seal it for more than 4 hours.

[0133] 5. Tablet pressing machine, 600mg per tablet.

[0134] 6. After passing the ...

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Abstract

The invention discloses a preparation method and a preparation agent of nano lipidosome compound IgY resisting Helicobacter pylori, Pylori bacteria as well as related enzymes and adhesion of Helicobacter pylori and Pylori bacteria. The preparation method comprises the following steps: S1, preparing compound antigens respectively; S2, screening laying poultry, performing immunizing, and selecting immunized eggs respectively; S3, preparing two specific compound IgY coarse extracts which are corresponding to the two compound antigens respectively; S4, preparing compound pure IgY; S5, performing nano treatment; S6, performing lipidosome treatment. The nano lipidosome compound IgY resisting Helicobacter pylori, Pylori bacteria as well as the related enzymes and the adhesion of Helicobacter pylori and Pylori bacteria, provided by the invention, can specificallyrecognize pathogen of Helicobacter pylori and Pylori bacteria and relevant enzymes and adhesion, can take effect after penetrating through mucous membrane of stomach and duodenum, can directly inhibit Helicobacter pylori and Pylori bacteria, can inhibit relevant enzymes and the adhesion of Helicobacter pylori and Pylori bacteria, can prevent Helicobacter pylori from adhering to and setting on the surface of the mucous membrane, and can achieve the purpose of curing.

Description

technical field [0001] The present invention relates to the prevention and treatment technology of peptic ulcer and chronic gastritis caused by Helicobacter pylori infection, in particular to a preparation method of nano-liposome anti-Helicobacter pylori and pylori and its related enzymes and adhesin compound IgY and its formulations. Background technique [0002] Helicobacter pylori infection is the main cause of peptic ulcer and chronic gastritis, and is also closely related to the occurrence of gastric cancer. The two Australian scientists Barry J.Marshall and J.Robin Warren who revealed this essence have been awarded the 2005 Nobel Prize in International Medicine. However, antibiotic-based treatment methods are currently used for ulcers and other diseases, and the adverse reactions are large, and the resistance rate of Helicobacter pylori to antibiotics continues to increase, resulting in an increasing failure rate of eradication therapy. According to relevant data, th...

Claims

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Application Information

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IPC IPC(8): A61K39/40A61K9/127A61P1/04
Inventor 包晟王长安曹天福吴娜包海威
Owner SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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