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A method for coupling extraction and fermentation to separate lactic acid in fermentation broth

A fermented liquid and extraction technology, applied in the field of bioengineering, can solve the problems of high cost, difficulty in removing bacteria, protein and glucose, and low efficiency, and achieve the effect of cheap source, reduced energy consumption, and simple process

Active Publication Date: 2015-10-28
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to provide a new method for separating lactic acid in fermented liquid for the problems of low efficiency, high cost, and difficult removal of bacteria, protein and glucose, etc.

Method used

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  • A method for coupling extraction and fermentation to separate lactic acid in fermentation broth
  • A method for coupling extraction and fermentation to separate lactic acid in fermentation broth
  • A method for coupling extraction and fermentation to separate lactic acid in fermentation broth

Examples

Experimental program
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Effect test

preparation example Construction

[0035] 2. Preparation of lactic acid fermentation broth

[0036] (1) Inoculate Lactobacillus rhamnosus into the seed medium, and cultivate at 37°C and 200rpm shaker for 24h;

[0037] (2) insert the bacterial culture liquid of step (1) into the fermentation medium at the inoculum size of 10% (v / v), and carry out fermentation, the stirring speed is 250r / min, the fermentation temperature is 37 ° C, and the fermentation process does not Aeration, using 5mol / L NaOH solution to control the pH value to 5.5, and fermenting for 28h to obtain lactic acid fermentation broth, the concentration of lactic acid in the fermentation broth is 76.95g / L, and the residual glucose concentration is 12g / L.

[0038] Pretreatment of lactic acid fermentation liquid: The lactic acid fermentation liquid obtained in the above 2 contains bacterial cells, and can be pretreated by flocculation, filtration, microfiltration or centrifugation to remove the bacterial cells to obtain lactic acid fermentation clear...

Embodiment 1

[0060] Embodiment 1 The influence of different organic solvents (extracting agents) on the separation effect of lactic acid in fermentation broth

[0061] Lactic acid was isolated from the lactic acid simulant as described in 3 above. Among them, the concentration of lactic acid in the lactic acid simulation solution was 150 g / L, and the pH was 5.5. Glucose is added to the lactic acid simulation solution and completely dissolved to obtain a sugar-containing mixed solution. Add different organic solvents (organic solvent types, as shown in Table 1) to the sugar-containing mixed solution, mix, adjust the pH of the mixed solution to 3.5, stand at room temperature to obtain two layers of water phase (lower phase) and organic phase (upper phase) In the extraction system with the coexistence of liquid phases, samples were taken to measure the concentration of lactic acid in the upper and lower phases, and the partition coefficient (K) and recovery rate (Y) of lactic acid were calcu...

Embodiment 2

[0066] Embodiment 2 The influence of different sugars on the separation effect of lactic acid in fermentation broth

[0067] Lactic acid was isolated from the lactic acid simulant as described in 3 above. Among them, the concentration of lactic acid in the lactic acid simulation solution was 150 g / L, and the pH was 5.5. Different kinds of sugars (types of sugars, as shown in Table 2) were added to the lactic acid simulant solution and completely dissolved to obtain a sugar-containing mixed solution. Add isopropanol to the sugar-containing mixed solution, mix, adjust the pH of the mixed solution to 3.5, and stand at room temperature to obtain an extraction system in which two liquid phases coexist in an aqueous phase (lower phase) and an organic phase (upper phase). The concentration of lactic acid in the phase and the lower phase, the partition coefficient (K) and the recovery rate (Y) of lactic acid were calculated, and the results are shown in Table 2. The mass percentages...

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Abstract

The invention provides a method for extracting, fermenting, coupling and separating lactic acid in fermentation liquor. The method comprises the following steps of dissolving sugar or / and inorganic salt in lactic acid fermentation liquor to obtain sugar-containing mixed liquor; adding an organic solvent into the sugar-containing mixed liquor, oscillating and mixing, regulating a pH value of the mixed liquor to 2-4, stewing or centrifuging at 15 DEG C-40 DEGC until separating phase to obtain an extracting system in which an upper-layer organic phase and a lower-layer water phase coexist; distilling and recycling the organic solvent under reduced pressure at a temperature lower than 50 DEG C to obtain lactic acid concentrated liquor; removing the organic solvent from the water phase, adding water to dilute until sugar concentration is 100g / L-250g / L, accessing thallus and fermenting the lactic acid. The method disclosed by the invention is simple to operate and low in separating energy consumption, realizes coupling of fermentation and separation; the water phase is directly used for fermenting without being recycled, so that emission of wastewater is reduced, and recovery rate of lactic acid is over 75%, and therefore, the method is a method for separating alcohol from fermentation liquor, which has industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to the separation technology of microbial fermentation liquid, and particularly relates to a method for coupling fermentation and separation of lactic acid in fermentation liquid by using extraction technology. Background technique [0002] As one of the three major organic acids recognized in the world, lactic acid is not only an important chemical raw material, but also an important fine chemical. It is widely used in food, medicine, agriculture, daily necessities, chemical industry and other fields. In particular, L-lactic acid with high optical purity has a huge market demand because it can be used as a precursor of biodegradable material L-polylactic acid. [0003] The production methods of lactic acid include three methods: chemical synthesis method, enzymatic method and microbial fermentation method. At present, the fermentation method is mainly used in industrial productio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C59/08C07C51/48C12P7/56C12R1/225
CPCC07C51/48C12P7/56C07C59/08
Inventor 孙亚琴鄢凌戴建英修志龙
Owner DALIAN UNIV OF TECH
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