Method for fermenting and producing DHA
A fermentation medium and by-product technology, applied in the field of fermentation and production of DHA, can solve problems such as poor oxidation stability, and achieve the effects of improving production efficiency, facilitating industrial scale production, and facilitating growth and product accumulation
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Embodiment 1
[0033] The present embodiment relates to a method for producing DHA by fermentation, comprising the following steps:
[0034] Step 1, prepare seed culture medium;
[0035] The components of the seed medium are, in every 1L medium, glucose 50.0g, yeast extract 5.0g, peptone 3.0g, MgSO 4 2.0g, KH 2 PO 4 2.0g, the balance is water; pH is 6.0, sterilized at 121°C for 30min;
[0036] Step 2, preparing fermentation medium;
[0037] (a) Glucose 100.0g / L, yeast extract powder 15.0g / L, chlorophyll 1g / L, VB 1 0.1g / L, VB 12 0.1g / L, VB 6 0.1g / L, MSG 8g / L, glutamic acid 4g / L, biotin 0.1g / L, β-carotene 0.1g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, Na 2 SO 4 12.0g / L, sea crystal 15g / L, the balance is water, and the pH is controlled at 5.5-6.5 during the fermentation process.
[0038] (b) Corn processing by-products 120g / L, glucose 20g / L, yeast extract 5g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, Na 2 SO 4 12.0g / L, sea crystal 15g / L, the balance is water, and the pH is natural during the ...
Embodiment 2
[0049] This embodiment relates to a method for producing DHA by fermentation, comprising the steps of:
[0050] Step 1, preparing seed culture medium;
[0051] The components of the seed medium are, in every 1L medium, glucose 50.0g, yeast extract 5.0g, peptone 3.0g, MgSO 4 2.0g, KH 2 PO 4 2.0g, the balance is water; pH is 6.0, sterilized at 121°C for 30min;
[0052] Step 2, preparing fermentation medium;
[0053] The components of the fermentation medium are as follows: every 1L medium contains 120g of corn processing by-products, 20.0g of glucose, 10.0g of yeast extract, MgSO 4 1.0g, KH 2 PO 4 3.0g, Na 2 SO 4 12.0g, sea crystal 15g, the balance is water; sterilize at 121°C for 30min, cool down for later use;
[0054] Step 3, shake flask seed cultivation and seed expansion cultivation;
[0055] Take a glycerol tube of 1 mL of Schizochytrium sp. frozen in a refrigerator at -80 °C, inoculate it into a 500 mL Erlenmeyer flask containing 200 mL of the above seed medium,...
Embodiment 3
[0061] This embodiment relates to a method for producing DHA by fermentation, comprising the steps of:
[0062] Step 1, preparing seed culture medium;
[0063] The components of the seed medium are, in every 1L medium, glucose 40.0g, yeast extract 4.0g, peptone 2.0g, MgSO 4 1.0g, KH 2 PO 4 1.0g, the balance is water; pH is 6.0, sterilized at 121°C for 30min;
[0064] Step 2, preparing fermentation medium;
[0065] The components of the fermentation medium are as follows: every 1L medium contains 80g of corn processing by-products, 10.0g of glucose, 5.0g of yeast extract, MgSO 4 0.5g, KH 2 PO 4 2.0g, Na 2 SO 4 5.0g, sea crystal 5g, the balance is water; sterilize at 121°C for 30min, cool for later use;
[0066] Step 3, shake flask seed cultivation and seed expansion cultivation;
[0067] Take a glycerol tube of 1 mL of Schizochytrium sp. frozen in a refrigerator at -80 °C, inoculate it into a 500 mL Erlenmeyer flask containing 200 mL of the above seed medium, and cult...
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