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Salidroside contained plant cultivation method and plant applications

A sedroside plant and plant technology, applied in the field of biotransformation, can solve problems such as low yield of salidroside, complicated chemical synthesis methods, and shortage of resources, and achieve the effects of protecting the liver, enhancing immunity, and protecting the cardiovascular system

Inactive Publication Date: 2014-08-27
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a cultivation method and plant application of salidroside-containing plants, so as to solve the problem of resource shortage caused by the low yield of salidroside and complicated chemical synthesis methods.

Method used

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  • Salidroside contained plant cultivation method and plant applications
  • Salidroside contained plant cultivation method and plant applications
  • Salidroside contained plant cultivation method and plant applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment one, get Convolvulaceae water spinach, add tyrosol in the water body of its growth, make its initial concentration be 10mmol / L, detect the concentration of tyrosol once every 3~5 days, when down to 8mmo / L (that is, when the initial concentration is 80%), add tyrosol to make the concentration reach 10mmol / L, and stop adding tyrosol when the culture reaches 5 days before harvest. After harvest, the content of salidroside (fresh weight) in water spinach can reach more than 0.20%.

[0046] Detection method of salidroside: adopt high-performance liquid chromatography, take 0.5g of the sample to be tested, add 25ml of 50% methanol, weigh it, heat and reflux for 30 minutes, let it cool, make up for the lost weight, filter, and use it as a test sample. Test solution. Take another salidroside reference substance, add 50% methanol to make a solution containing 0.05mg per 1ml, as the reference substance solution. Octadecylsilane bonded silica gel is used as filler; me...

Embodiment 2

[0047] Embodiment 2, get Compositae lettuce lettuce seeds, soak the seeds in clear water, sow in the sandy soil of the cultivation tray, keep moisture, after the cotyledons grow 2cm from the seeds, spray the seedlings with 1 / 10 concentration nutrient solution until 2 pairs of true leaves grow Afterwards, transfer to 1 / 2 concentration of nutrient solution for culture, monitor the EC value (conductivity) of the culture solution every day, when the EC value drops to half of the initial EC value, change the full concentration nutrient solution for culture (add 8mmol / L tyrosol), and later when the EC value drops to half, replace the full-concentration nutrient solution containing tyrosol, harvest when the seedlings reach 20 cm or more, and measure the content of salidroside, and its content (fresh weight) can reach up to More than 0.3%.

[0048] The nutrient solution adopts Hoagland’s formula, which is as follows: calcium nitrate tetrahydrate 945mg / L, potassium nitrate 607mg / L, am...

Embodiment 3

[0049] Embodiment three, get Compositae plant lettuce seedling, cultivate according to the conventional soilless culture method, after growing 3~5 true leaves, add tyrosol in the culture solution so that its concentration is 5mmol / L, every 3~5 leaves On day 15 (the initial interval is 15 days, and then the interval is gradually shortened to 3 days), tyrosol is added to the culture medium to make the concentration 5mmol / L, and the addition of tyrosol is stopped 3 days before harvest, so as to allow the complete conversion of tyrosol, and harvest After the lettuce, the content of salidroside was determined according to the above method, and its content (fresh weight) could reach more than 0.15%.

[0050] Commonly used green leafy vegetable nutrient solution formula is as follows: calcium nitrate tetrahydrate 1260mg / L, potassium sulfate 250mg / L, ammonium dihydrogen phosphate 350mg / L, magnesium sulfate heptahydrate 537mg / L, ammonium sulfate 237mg / L, boric acid 2.86mg / L, borax 4.5...

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Abstract

The invention provides a salidroside contained plant cultivation method and plant applications. The salidroside contained plant cultivation method comprises adding exogenous butyl alcohol into plant growing water or culture solution, enabling the initial concentration of the plant growing water or culture solution to be 0.1 to 100 mmol per liter; adding the butyl alcohol as supplement or not with interval of certain time; enabling the butyl alcohol to be converted into the salidroside under the function of related enzymes in cells after being absorbed by a plant; accordingly enabling the plant to obtain the physiological pharmacological activity as the salidroside. The plant can be directly used, processed into health food, medicine, food additives and the like or be served as raw materials for extraction of the salidroside.

Description

technical field [0001] The invention relates to the technical field of biotransformation, in particular to a cultivation method for cultivating plants containing salidroside and plant application. Background technique [0002] Salidroside (salidroside) is the most important medicinal ingredient of Crassulaceae (Crassulaceae) Rhodiola (Rhodiola L.) plants. Protect nerve cells, protect the liver, anti-oxidation, anti-tumor, anti-radiation and other specific effects. It has extremely important application value in military, aerospace, sports and health care medicine. In recent years, there are more and more types of products such as pharmaceuticals, beverages, food and cosmetics produced with it as the main raw material, and people's demand for salidroside is increasing. [0003] Rhodiola plants are the main source of extracting salidroside, but wild resources are scarce, and the content of salidroside is very low, such as the most commonly used Rhodiola alpine and Rhodiola g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G7/06A23L1/29A23L1/30C07H15/18C07H1/08A61K36/39A61K36/31A61K36/81A61K36/28A61P9/10A61P25/28A61P9/00A61P39/00A61P37/04A61P25/00A61P1/16A61P39/06A61P35/00A61P17/16A23L33/00
Inventor 谭朝阳张倩彭菲于静崔培梧鲁耀邦林艳
Owner HUNAN UNIV OF CHINESE MEDICINE
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