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Fresh water chlorella showing heterotrophic growth characteristics with high-concentration CO2

A technology of growth characteristics and chlorella, applied in the field of microorganisms, can solve the problems of high production cost of microalgae oil and greenhouse effect, etc.

Active Publication Date: 2014-08-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a strain of freshwater chlorella that can utilize high concentrations of CO 2 Accumulate a large amount of biomass and oil, and the maximum specific growth rate, average daily carbon fixation rate, light energy conversion rate, and carbon oil conversion rate are significantly improved and are close to those in heterotrophic culture, and high-concentration CO 2 At this time, the algae strain showed some heterotrophic growth characteristics, so as to solve the problems of high production cost of microalgae oil and greenhouse effect.

Method used

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  • Fresh water chlorella showing heterotrophic growth characteristics with high-concentration CO2
  • Fresh water chlorella showing heterotrophic growth characteristics with high-concentration CO2
  • Fresh water chlorella showing heterotrophic growth characteristics with high-concentration CO2

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Experimental program
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Effect test

Embodiment 1

[0055] 1. Sample collection

[0056] Water samples containing green algae were collected from a freshwater lake in July 2010.

[0057] 2. Efficient use of high-concentration CO 2 Isolation and screening of algal strains

[0058] Separation step: use the dilution coating plate method to separate the algae species. The collected water samples were transferred to TAP liquid medium that had been autoclaved (121°C, high-pressure steam sterilization for 20 minutes), placed in a light incubator for enrichment culture, and then diluted and coated in solid TAP medium. After a period of time, single colonies on the plate can be picked out to obtain pure algal species.

[0059] Screening steps: Inoculate the obtained pure algal strains on a 24-well plate, place in a closed glass box, and fill the glass box with sterile 10% CO 2 , use 680nm to detect the growth situation, select the algae strains with faster growth, and measure their growth in high concentration CO 2 lower fat conten...

Embodiment 2

[0076] In order to investigate the high concentration of CO 2 Under conditions, the energy conversion efficiency of C.sorokiniana CS-1, the culture medium 1 that adopts is the BG-11 medium that removes carbon source, the formula (per liter) of BG-11 medium is as follows: 1500mg NaNO 3 , 40mg K 2 HPO 4 ·3H 2 O, 75mg MgSO 4 ·7H 2 O, 36mg CaCl 2 ·7H 2 O, 1mL trace element solution A 5 . Trace element solution A 5 The formula (per liter) is as follows: 2860mg H 3 BO 4 , 1810mg MnCl 2 4H 2 O, 222mg ZnSO 4 ·7H 2 O, 391 mg Na 2 MoO 4 , 79mg CuSO 4 ·5H 2 O, 49.47mg Co(NO 3 ) 2 ·6H 2 O; medium 2 is to add 16g L to the BG-11 medium that removes carbon source -1 of glucose and 6g·L -1 Soybean peptone was cultured heterotrophically, the pH value was 7-8, the medium was divided into 250mL ventilated culture bottles, the culture volume was 150mL, and sterilized at 121°C for 20min.

[0077] Inoculate fresh C.sorokiniana CS-1 with volume fraction of 1v / v% in the cultur...

Embodiment 3

[0083] In order to examine the CO 2 We analyzed the algae oil obtained in Example 2 for the effect on the polyunsaturated fatty acid concentration of C.sorokiniana CS-1. The obtained results are shown in Table 1. It can be seen from the table that C.sorokiniana CS-1 can utilize high concentration CO 2 Polyunsaturated fatty acid α-linolenic acid is accumulated while accumulating biomass and oil content, while the content of α-linolenic acid in C. 2 It is a better culture method to induce C.sorokiniana CS-1 to exhibit heterotrophic characteristics while maintaining the content of α-linolenic acid, a high-value by-product of Chlorella.

[0084] The methylation of algae oil: the algae oil that embodiment 2 obtains is used CHCl 3 Dissolve, transfer to 1.5mL Agilent glass bottle, add 1mL 1M methanolic sulfuric acid solution, fill with N 2 Seal, react at 100°C for 1 hour, cool naturally, add 200 μL deionized water, mix well, extract 3 times with 200 μL n-hexane, combine the organ...

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Abstract

The invention discloses fresh water chlorella showing heterotrophic growth characteristics with high-concentration CO2. The fresh water chlorella is classified and named as Chlorella sorokiniana, the bacterial strain number is CS-1, the culture preservation register number is CGMCC No. 9215, and the preservation date is May 5, 2014. The invention further discloses the application of the chlorella in grease production. The application can efficiently keep high-concentration CO2 constant in exhaust gas environment, the production cost of the microalgae grease is thus reduced, and superior production algae is provided for biodiesel preparation. The light energy conversion rate of the cells of the chlorella is increased and is close to the chemical energy conversion rate when the high-concentration CO2 serves as a substrate, the cells show part of heterotrophic growth characteristics, and namely, when the high-concentration CO2 is fed into the cells, the specific growth rate, the grease synthesis rate and the energy utilization efficiency of the chlorella are obviously increased and are close to those of the chlorella under the heterotrophic culture condition.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to a kind of microalgae, in particular to a strain utilizing high-concentration CO 2 Freshwater Chlorella exhibiting partially heterotrophic growth characteristics. Background technique [0002] The light radiation density on the earth's surface is about 985.7W / m 2 , and the light energy absorbed by higher plants through photosynthesis only accounts for 0.2-2% of solar radiation energy, and the lower photosynthetic efficiency of higher plants leads to the waste of a large amount of solar energy. In recent years, microalgae have attracted much attention because of their advantages such as high photosynthetic efficiency, fast cell growth, and ability to accumulate a large amount of oil and convert it into bioenergy. Although the photosynthetic efficiency of microalgae is much higher than that of higher plants, the light energy absorbed by them only accounts for 6% of the total sola...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02P20/141
Inventor 孙芝兰陈以峰窦晓
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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