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Electrochemical-luminescence sensor for quickly detecting diphenhydramine hydrochloride by high sensitivity and preparation method thereof

A diphenhydramine hydrochloride, sensitive detection technology, applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by making materials undergo chemical reactions, etc., can solve the problem of difficult time and space control, chemiluminescence Difficult to reuse reagents, uneven mixing of solutions, etc., to achieve good electrochemical performance and biocompatibility, reasonable detection recovery, and good electrical conductivity

Inactive Publication Date: 2014-08-27
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with other analysis methods, the electrochemiluminescence method overcomes some chemiluminescent reagents that are not easy to store or unstable under specific conditions, it is difficult to achieve time and space control, it is difficult to reuse chemiluminescent reagents, and the solution is not mixed uniformly. Relatively poor reproducibility and other disadvantages

Method used

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  • Electrochemical-luminescence sensor for quickly detecting diphenhydramine hydrochloride by high sensitivity and preparation method thereof
  • Electrochemical-luminescence sensor for quickly detecting diphenhydramine hydrochloride by high sensitivity and preparation method thereof
  • Electrochemical-luminescence sensor for quickly detecting diphenhydramine hydrochloride by high sensitivity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Ru(bpy) 3 2+ Preparation of / MWCNT-Cys-AuNPs / Nafion Modified Electrode

[0027] Weigh 30mg of multi-walled carbon nanotubes into 60mL H 2 SO 4 / HNO 3(3:1) mixed acid was continuously ultrasonicated for 16h, then centrifuged and washed until the pH value was close to 7, and dried in an oven at 60°C. Take 15 mg of the above interrupted carbon nanotubes and dissolve them in 5 mL of water, then add 20 mg of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), 20 mg of N-hydroxy Succinimide (NHS) and 40 mg of cysteine ​​(L-cystine) were shaken uniformly by ultrasound, adjusted to a pH between 7 and 8 with 0.1 mol NaOH, shaken overnight to form MWCNT-Cys complexes, centrifuged, washed and dry. Get 100mL of 0.01% chloroauric acid solution and heat it in an Erlenmeyer flask. After the solution boils, add 2mL trisodium citrate (0.02g / mL) solution quickly and continue to boil for 15 minutes. After natural cooling, the gold nanoparticle solution i...

Embodiment 2

[0029] Example 2: Ru(bpy) 3 2+ Qualitative detection of electrochemiluminescence of DPH by / MWCNT-Cys-AuNPs / Nafion modified electrode

[0030] Ru(bpy) 3 2+ / MWCNT-Cys-AuNPs / Nafion modified electrode is placed in 4mL0.1MPBS (pH=7.4) buffer solution, the redox current generated is very small and the light intensity is relatively weak, which is a Ru(bpy) 3 2+ Since the electrochemiluminescence process of annihilation, after adding 0.25mM DPH, the intensity of electrochemiluminescence is obviously enhanced, showing a typical co-reactant electrochemiluminescence process ( figure 2 ), and the modified electrode exhibited good electrochemiluminescent properties for DPH.

Embodiment 3

[0031] Example 3: Ru(bpy) 3 2+ Quantitative detection of electrochemiluminescence of DPH by / MWCNT-Cys-AuNPs / Nafion modified electrode

[0032] Ru(bpy) 3 2+ / MWCNT-Cys-AuNPs / Nafion modified electrode placed in PBS (pH=9.0) buffer solution containing a certain amount of DPH, with the continuous addition of DPH, the intensity of electrochemiluminescence increases continuously, when DPH reaches a certain concentration, the light intensity There is no longer a noticeable increase. The linear range of the sensor to detect DPH is 2×10 by plotting the concentration of DPH with the intensity of electrochemiluminescence. -8 M~7.5×10 -4 M, the detection limit is 6.7nM (S / N=3) ( image 3 ). It was placed in PBS (pH=9.0) containing different concentrations of DPH, showing better stability ( Figure 4 ), the experimental results show that the prepared sensor has the advantages of low detection limit, high sensitivity, wide linear range, and stability.

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Abstract

The invention discloses an electrochemical-luminescence sensor for quickly detecting diphenhydramine hydrochloride by high sensitivity and a preparation method thereof. The preparation method comprises the following steps: connecting and synthesizing carbon nano tubes and nano gold into a WMCNT-CYs-AuNPs nano composite material by cysteine, modifying a WMCNT-CYs-AuNPs / Nafion nano composite membrane on the surface of a glassy-carbon electrode, then utilizing the nano composite membrane to immobilize a luminescence reagent terpyridyl ruthenium (Ru(bpy)32+), constructing the electrochemical-luminescence sensor based on Ru(bpy)32+ / WMCNT-CYs-AuNPs / Nafion. The synthesis method is simpler and does not need expensive instruments; the sensor constructed by the invention has the characteristics of high sensitivity, low detection limit, wide linear range, and good stability and reproducibility and the like. The electrochemical-luminescence sensor is reasonable in recovery rate and obtains a better result for detecting market diphenhydramine injection.

Description

technical field [0001] The invention relates to the field of electrochemical sensors, in particular to an electrochemiluminescence sensor for rapid and highly sensitive detection of diphenhydramine hydrochloride and a preparation method thereof. Background technique [0002] Diphenhydramine, also known as Benadryl, Konamine, and Benazol, is a white crystalline powder with an odorless, bitter taste. It is a derivative of ethanolamine, which can compete with the histamine released from tissues for the H1 receptor on effector cells, thereby preventing allergic attacks, and also has sedative, hypnotic and antitussive effects. It is mainly used clinically to treat skin and mucous membrane allergies, such as urticaria, angioneurotic edema, allergic dermatitis, etc. It can also be used for acute allergic reactions, prevention and treatment of motion sickness and seasickness, hypnosis and preoperative administration and 1% diphenhydramine Clear solution can be used as a local anest...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N21/76
Inventor 贾能勤苗冲冲吴贝娜
Owner SHANGHAI NORMAL UNIVERSITY
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